A collection of www-links for
The Yeast Two-Hybrid Screen

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The first use of the two-hybrid system is attributed to Song and Fields (1989). Roger Brent's lab (Harvard University, Boston) has developed a nice variant of the system and he has a lot of www-information on two hybrid screens, and Stephen J. Elledge's lab (Baylor College of Medicine, Houston) has contributed a lot to the development of useful systems.

• Interaction trap cloning with yeast.
  A draft manuscript by Russell L. Finley Jr. and Roger Brent to appear in "Gene Probes - A practical Approach" published by Oxford University Press. Here are many protocols and tidbits on the methods.
• Information on yeast strains in use.
• Applications of interaction traps/ two hybrid systems to biotechnology research
  This is a draft version of a manuscript by Andrew R. Mendelsohn and Roger Brent that is to appear in the October 1994 issue of Current Opinions in Biotechnology. It reviews the use of interaction technology in biotechnology.

Other useful stuff:

• The Saccharomyces Information Resource and the Saccharomyces Genome Database at Stanford.
• The yeast virtual library
• Yeast cloning vectors
• The BioSci Newsgroup for yeast. or search in the BioSci USENET archives.
• A collection of LexA fusion plasmids (Erica Golemis, Fox Chase Cancer Center, N.J.)
• Recovery of plasmids from yeast (method for S. pombe - but mentions E. coli JA226) Fission Yeast Handbook by Frans Hochstenbach, (University of Amsterdam).
• Tabulation of typical false positives obtained in two-hybrid screens - and here is the LONG LIST (Erica Golemis, Fox Chase Cancer Center, N.J.).
• pAS2 bait vector (gb:U30496)

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Clontech provides tools, vectors, strains and libraries for two-byrdid screen:

• The original Matchmaker system
• The new version Matchmaker 2
• New Libraries
• The vectors:
  • pGBT9 and it's sequence .
  • pGAD424 and it's sequence .
  • pGAD10 and it's sequence .
• E. coli strain KC8 (hsdR, leuB600, trpC9830, pyrF::Tn5, hisB463, lacDX74, strA, galU,K) can be used (according to Clontech) to rescue Trp⁺, Leu⁺ and His⁺ plasmids from yeast (see Struhl et al. 1979).

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This page was generated by Rein Aasland December 2. 1995 Last updated 24. January 1999