Molecular and Cellular Methods in Immunology
Course offered :
- Current semester
- Next semester
Course offered by
| Number of credits | 10 |
| Course offered (semester) | Spring. Note: course will only be arranged if at least 2 students sign up for the course. Maximum capacity is 6 students. |
| Schedule | Schedule |
| Reading list | Reading list |
Language of Instruction
English.
Pre-requirements
Need bachelordegree
Some background in cellular and molecular biology. Experience with basic laboratory techniques such as pipetting and centrifugation. Priority will be given to students having taken the related course Human Immunology (HUIMM903/HUIMM303).
Learning Outcomes
Knowledge
Upon completing the course, the students should know the most commonly used immunological methods and understand the aim and theory behind those techniques.
Skills
Upon completing the course, the students should be able to plan and perform experiments using the most commonly used immunological methods.
Competence
Upon completing the course, the students should be able to apply their knowledge and skills to engage in further tasks related to research.
Contact Information
Silke Appel (silke.appel@gades.uib.no)
Course offered (semester)
Spring. Note: course will only be arranged if at least 2 students sign up for the course. Maximum capacity is 6 students.
Language of Instruction
English.
Course Unit Level
Master.
Aim and Content
Aim:
Introduction to some general methods used in immunological research by using them actively in the lab. The practical part of the course should enable the students to understand methods used in scientific papers introduced in the theoretical part of the course. In addition, the methods used will be introduced in seminars by the course leaders.
Contents:
Theoretical part:
-The course leaders will give a theoretical introduction into some of the most commonly used immunological methods.
-Student colloquium: The students present 1-2 papers each, discussing the aim and methods used in the forum.
Practical part:
-Calculation of molarities, preparation of buffers
- Isolation of leukocytes from blood using density gradient centrifugation. Isolation and cultivation of monocytes (cell culture), in vitro generation of macrophages.
- Western blot analysis of signaling pathways using stimulated cells.
- ELISA. Quantificatin of cytokins produced by the cells.
- Immunostaining. Visualisation using fluorescently labeled secondary antibody.
- Quantification of copy number using PCR: Comparison of standard PCR with quantitative real time PCR.
Learning Outcomes
Knowledge
Upon completing the course, the students should know the most commonly used immunological methods and understand the aim and theory behind those techniques.
Skills
Upon completing the course, the students should be able to plan and perform experiments using the most commonly used immunological methods.
Competence
Upon completing the course, the students should be able to apply their knowledge and skills to engage in further tasks related to research.
Pre-requirements
Need bachelordegree
Some background in cellular and molecular biology. Experience with basic laboratory techniques such as pipetting and centrifugation. Priority will be given to students having taken the related course Human Immunology (HUIMM903/HUIMM303).
Recommended previous knowledge
Some background in cellular and molecular biology. Experience with basic laboratory techniques such as pipetting and centrifugation. Priority will be given to students having taken the related course Human Immunology (HUIMM903/HUIMM303).
Teaching Methods
Articles, textbooks
Theoretical introduction to the lab (1.5 h)
10 days in the lab (60 h) and
6 colloquium (9 h)
The course gives 10 credits upon completion.
Assessment methods
Oral presentation of given articles and written lab report (composed of Introduction, Materials and Methods, Results, Discussion). Both have to be approved by the course leader.
Reading List
To be announced.
Place of Teaching
Bergen Research School in Inflammation, Broegelmann Research Laboratory
Laboratory building, 5th floor, Department of clinical science
Contact Information
Silke Appel (silke.appel@gades.uib.no)