Methods in Neurophysiology

Study facts

Course codeBMED340B
ECTS credits5
Teaching semesterSpring
Number of semesters1
Teaching language
Study levelPostgraduate Courses
Belongs toThe Department of Biomedicine


Contact Information

Department of Biomedicine

(+47) 55 58 64 39 / 55 58 64 40 / 55 58 60 95

Course coordinator:

Margaret Veruki,

Objectives and Content

Aim is to give the students a theoretical and a practical understanding of common methods used in neuroscience research.


  • Hippocampus as a model for studies of synaptic plasticity in the CNS, principles of in vivo recording of the field potentials and induction of LTP. Glutamate synapses in the hippocampus: pre- and postsynaptic structure, dendritic transport and changes due to the induction of LTP.
  • Theoretical introduction to the methods, apparatus, data collection and analysis in fMRI studies in humans and animals. Practical demonstration of fMRI recording.
  • Principles of the patch-clamp technique and demonstration of in vitro electrophysiological recordings in tissue slices from the CNS. Focus on ligand-gated and voltage-gated ion channels.
  • Introduction to stuctural and functional imaging of intact neuronal preparations using multiphoton excitation microscopy. Demonstration of single neuron morphological reconstructions.
  • Introduction to the physiology of pain: receptors and circuits for pain, structure of the spinal cord, neurochemistry, plasticity, electrophysiological methods for in vivo recording from spinal cord neurons, experimental setup, recording equipment, data collection. Demonstration of single unit recording in the spinal cord.

The course will run for the last time in the spring semester 2015.

Subject Overlap


Teaching Methods and Extent of Organized Teaching

Lectures, laboratory demonstrations.

Semester of Instruction

Spring. The course admits a limited number of students. 

Learning Outcomes

After completion of the course, the student should be able to:

  • Explain commonly used methods in neuroscience.
  • Understand the theoretical background for different types of electrophysiological recordings.
  • Plan and conduct simple experiments using relevant methods in study of the nervous system.
  • Interpret and evaluate the results of a simple experiment.

Required Previous Knowledge

Neurobiology (equivalent to MED1NEVRO), Biology, molecular biology or equivalent on bachelor level, preferably completed with a degree.

Forms of Assessment

Semester assignment in a subject from the course chosen by each student individually. Oral presentation.

Grading Scale


Course Evaluation

Written evaluation on My Space/Mi side.