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Ida Helene Steen

Associate Professor, Centre for Geobiology, Theme Leader
  • E-mailIda.Steen@uib.no
  • Phone+47 55 58 83 75
  • Visitor Address
    Thormøhlensgt. 53 A/B
  • Postal Address
    Postboks 7803
    5020 Bergen

Current PhD and master students  (main or co- supervisor)

PhD-student Anders Schouw (UiB, Biogoldmine).Biogenic conversion of higher hydrocarbons to methane - New isolates, functional insights and models.

PhD-student Angéle Abboud (UiB, MBI, Biogoldmine). Mining of sequencing data for novel enzymes: development and application of a bioinformatic expert system.

PhD-student Sven LeMoine Bauer (UiB, CGB). An ecological analysis of different microbial and viral communities from deep-sea hydrothermal vents.

PhD-student Jan Vander Roost (UiB, CGB). Iron oxidizing bacteria at the Jan Mayen hydrothermal vent field.

PhD-student Hasan Arsin (UiB, NorZymeD). Industrial application of proteases deriving from extreme environments.

Graduated

Msc Andreas Gilje Sjøberg. Physiological characterization of a novel strain within Rhodobacteraceae, isolated from a biofilm sample on a barite chimney at the Loki´s Castle Vent Field. - 2016

PhD Juliane Wissuwa. The Arctic mid-Ocean Ridge Vent Fields - A valuable Resource for Marine Bioprospecting? -2016

MSc Sepideh Mostafavi.Metagenome-based analysis of the functional potential of a marine sediment community dominated by ANME-2c. -2015

MSc Sophie Radeke. Diversity, function and interactions of anaerobic methanotrophs in Loki ́s Castle Vent Field. -2015

MSc Victoria Røyseth. Isolering og fysiologisk karakterisering av Marinitoga sp. stamme 2PyrY55-1ᵀ. -2015

PhD William P. Hocking (UiB). Energy conservation mechnisms in the sulfate respiring  archaeon Archaeoglobus fulgidus VC16-assessed by transcriptome analyses and comparative genomics. -2015

PhD Anita-Elin Fedøy (UiB). Structure-function analysis of isocitrate dehydrogenase subfamily II: Adaptations to hot and cold –2014

PhD Steffen L Jørgensen (UiB, CGB). Linking microbial community structure and geochemistry in deep-sea sediments - 2013

PhD Irene Roalkvam (UiB, VISTA). Diversity, stratification and in situ metabolism of anaerobic methanotrophic archaea in Nyegga cold seeps  - 2012

MSc Elisabeth Machteld Biersma. Microbial diversity and function associated with a black smoker from Loki´s Castle vent field. - 2012

MSc Carina Magnussen. Genekspresjon av karbonmonoksid dehydrogenase/acethyl-CoA syntase komplekset og sat-ORF2-aprBA operonet i Arhcaeoglobus fulgidus. - 2010

MSc Irene Roalkvam. Proteomikkstudier av den sulfatreduserende hypertermofile arkéen Archaeoglobus fulgidus - 2008

MSc Solveig Hoem. Diversitet og utbredelse av sulfatreduserende og svoveloksiderende prokaryoter i hydrothermale miljø.  - 2008

PhD Runar Stokke (UiB). Isocitrate dehydrogenase from extremophiles: Molecular adaptations to high temperatures. - 2007

MSc William P. Hocking. Kinetic characterization revealing a possible NADP substrate inhibition mechanism in isocitrate dehydrogenase of the hyperthermophilic archaeon Aeropyrum pernix. - 2007

Journal articles
  • Wissuwa, Juliane; Le Moine Bauer, Sven; Steen, Ida Helene; Stokke, Runar. 2017. Complete genome sequence of Lutibacter profoundi LP1T isolatet from an Arctic deep-sea hydrothermal vent system. Standards in Genomic Sciences. 12. doi: 10.1186/s40793-016-0219-x
  • Le Moine Bauer, Sven; Roalkvam, Irene; Steen, Ida Helene; Dahle, Håkon. 2016. Lutibacter profundi sp. nov., isolated from a deep-sea hydrothermal system on the Arctic Mid-Ocean Ridge and emended description of the genus Lutibacter. International Journal of Systematic and Evolutionary Microbiology. 66: 2671-2677. doi: 10.1099/ijsem.0.001105
  • Schouw, Anders; Eide, Tove Leiknes; Stokke, Runar; Pedersen, Rolf B.; Steen, Ida Helene; Bødtker, Gunhild. 2016. Abyssivirga alkaniphila gen. nov., sp. nov., an alkane-degrading, anaerobic bacterium from a deep-sea hydrothermal vent system, and emended descriptions of Natranaerovirga pectinivora and Natranaerovirga hydrolytica. International Journal of Systematic and Evolutionary Microbiology. 66: 1724-1734. doi: 10.1099/ijsem.0.000934
  • Steen, Ida Helene. 2016. Et hav av bakterier er et hav av muligheter. Bergens Tidende.
  • Steen, Ida Helene. 2016. Norske varmekilder i dyphavet - en underfundig verden full av spennende livsformer. Årbok for Universitetsmuseet i Bergen.
  • Steen, Ida Helene; Dahle, Håkon; Stokke, Runar; Roalkvam, Irene; Daae, Frida Lise; Rapp, Hans Tore; Pedersen, Rolf B.; Thorseth, Ingunn Hindenes. 2016. Novel barite chimneys at the Loki´s Castle vent field shed light on key factors shaping microbial communities and functions in hydrothermal systems. Frontiers in Microbiology. 6:1510. doi: 10.3389/fmicb.2015.01510
  • Steinsbu, Bjørn Olav; Røyseth, Victoria; Thorseth, Ingunn Hindenes; Steen, Ida Helene. 2016. Marinitoga arctica sp. nov., a thermophilic, anaerobic heterotroph isolated from the Soria Moria vent field on the Arctic Mid-Ocean Ridge. International Journal of Systematic and Evolutionary Microbiology. doi: 10.1099/ijsem.0.001472
  • Tvedt, Morten Walløe; Eijsink, Vincentius Gerardus Henricus; Steen, Ida Helene; Strand, Roger; Rosendal, Guri Kristin. 2016. The missing link in ABS: The relationship between resource and product. Environmental Policy and Law. 46: 227-237.
  • Wissuwa, Juliane; Stokke, Runar; Fedøy, Anita-Elin; Lian, Kjersti; Smalås, Arne O.; Steen, Ida Helene. 2016. Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site. Standards in Genomic Sciences. 11:16: 1-12. doi: 10.1186/s40793-016-0137-y
  • Dahle, Håkon; Økland, Ingeborg Elisabet; Thorseth, Ingunn Hindenes; Pedersen, Rolf B.; Steen, Ida Helene. 2015. Energy landscapes shape microbial communities in hydrothermal systems on the Arctic Mid-Ocean Ridge. The ISME Journal. 9: 1593-1606. doi: 10.1038/ismej.2014.247
  • Hocking, William Peter; Roalkvam, Irene; Magnussen, Carina; Stokke, Runar; Steen, Ida Helene. 2015. Assessment of the carbon monoxide metabolism of the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus VC-16 by comparative transcriptome analyses. Archaea. 2015:235384. 12 pages. doi: 10.1155/2015/235384
  • Roalkvam, Irene; Bredy, Florian; Baumberger, Tamara; Pedersen, Rolf B.; Steen, Ida Helene. 2015. Hypnocyclicus thermotrophus gen. Nov., sp. nov. isolated from a microbial mat in a hydrothermal vent field. International Journal of Systematic and Evolutionary Microbiology. 65: 4521-4525. doi: 10.1099/ijsem.0.000606
  • Roalkvam, Irene; Drønen, Karine; Stokke, Runar; Daae, Frida Lise; Dahle, Håkon; Steen, Ida Helene. 2015. Physiological and genomic characterization of Arcobacter anaerophilus IR-1 reveals new metabolic features in Epsilonproteobacteria. Frontiers in Microbiology. 6. doi: 10.3389/fmicb.2015.00987
  • Stokke, Runar; Dahle, Håkon; Roalkvam, Irene; Wissuwa, Juliane; Daae, Frida Lise; Tooming-Klunderud, Ave; Thorseth, Ingunn Hindenes; Pedersen, Rolf B.; Steen, Ida Helene. 2015. Functional interactions among filamentous Epsilonproteobacteria and Bacteroidetes in a deep-sea hydrothermal vent biofilm. Environmental Microbiology. 17: 4063-4077. doi: 10.1111/1462-2920.12970
  • Drønen, Karine; Roalkvam, Irene; Beeder, Janiche; Torsvik, Terje; Steen, Ida Helene; Skauge, Arne; Liengen, Turid. 2014. Modeling of heavy nitrate corrosion in anaerobe aquifer injection water biofilm: A case study in a flow rig. Environmental Science and Technology. 48: 8627-8635. doi: 10.1021/es500839u
  • Hocking, William Peter; Stokke, Runar; Roalkvam, Irene; Steen, Ida Helene. 2014. Identification of key components in the energy metabolism of Archaeoglobus fulgidus by transcriptome analyses. Frontiers in Microbiology. 5:95. doi: 10.3389/fmicb.2014.00095
  • Urich, Tim; Lanzén, Anders; Stokke, Runar; Pedersen, Rolf B.; Bayer, Christoph; Thorseth, Ingunn Hindenes; Schleper, Christa Maria; Steen, Ida Helene; Øvreås, Lise. 2014. Microbial community structure and functioning in marine sediments associated with diffuse hydrothermal venting assessed by integrated meta-omics. Environmental Microbiology. 16: 2699-2710. doi: 10.1111/1462-2920.12283
  • Dahle, Håkon; Roalkvam, Irene; Pedersen, Rolf B.; Thorseth, Ingunn Hindenes; Steen, Ida Helene. 2013. The versatile in situ gene expression of an Epsilonproteobacteria-dominated biofilm from a hydrothermal chimney. Environmental Microbiology Reports. 5: 282-290. doi: 10.1111/1758-2229.12016
  • Stokke, Runar; Hocking, William Peter; Steinsbu, Bjørn Olav; Steen, Ida Helene. 2013. Complete genome sequence of the thermophilic and facultatively chemolithoautotrophic sulfate reducer Archaeoglobus sulfaticallidus PM70-1T. Genome Announcements.
  • Jørgensen, Steffen Leth; Hannisdal, Bjarte; Lanzén, Anders; Baumberger, Tamara; Flesland, Kristin; Fonseca, Rita; Øvreås, Lise; Steen, Ida Helene; Thorseth, Ingunn Hindenes; Pedersen, Rolf B.; Schleper, Christa Maria. 2012. Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge. Proceedings of the National Academy of Sciences of the United States of America. 109: E2846-E2855. doi: 10.1073/pnas.1207574109
  • Leiros, Hanna-Kirsti S.; Fedøy, Anita-Elin; Leiros, Ingar; Steen, Ida Helene. 2012. The complex structures of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila support a new active site locking mechanism. FEBS Open Bio.
  • Ray, Jessica Louise; Dondrup, Michael; Modha, Sejal; Steen, Ida Helene; Sandaa, Ruth-Anne; Clokie, Martha. 2012. Finding a needle in the virus metagenome haystack - micro-metagenome analysis captures a snapshot of the diversity of a bacteriophage armoire. PLoS ONE. 7. 13 pages. doi: 10.1371/journal.pone.0034238
  • Roalkvam, Irene; Dahle, Håkon; Chen, Yifeng; Jørgensen, Steffen Leth; Haflidason, Haflidi; Steen, Ida Helene. 2012. Fine-scale community structure analysis of ANME in Nyegga sediments with high and low methane flux. Frontiers in Microbiology. 3: 1-13. doi: 10.3389/fmicb.2012.00216
  • Stokke, Runar; Roalkvam, Irene; Lanzén, Anders; Haflidason, Haflidi; Steen, Ida Helene. 2012. Integrated metagenomic and metaproteomic analyses of an ANME-1-dominated community in marine cold seep sediments. Environmental Microbiology. 14: 1333-1346. doi: 10.1111/j.1462-2920.2012.02716.x
  • Roalkvam, Irene; Jørgensen, Steffen Leth; Chen, Yifeng; Stokke, Runar; Dahle, Håkon; Hocking, William Peter; Lanzén, Anders; Haflidason, Haflidi; Steen, Ida Helene. 2011. New insight into stratification of anaerobic methanotrophs in cold seep sediments. FEMS Microbiology Ecology. 78: 233-243. doi: 10.1111/j.1574-6941.2011.01153.x
  • Karlström, Mikael; Chiaraluce, Roberta; Giangiacomo, Laura; Steen, Ida Helene; Birkeland, Nils-Kåre; Ladenstein, Rudolf; Consalvi, Valerio. 2010. Thermodynamic and kinetic stability of a large multi-domain enzyme from the hyperthermophile Aeropyrum pernix. Extremophiles. 14: 213-223. doi: 10.1007/s00792-009-0300-0
  • Siltberg-Liberles, Jessica; Steen, Ida Helene; Svebak, Randi Myrseth; Martinez, Aurora. 2008. The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum. Gene. 427: 86-92. doi: 10.1016/j.gene.2008.09.005
  • Fedøy, Anita-Elin; Fedoy, AE; Yang, Nannan; Martinez, A; Leiros, Hanna-Kirsti S.; Steen, IH. 2007. Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability. Journal of Molecular Biology. 372: 130-149. doi: 10.1016/j.jmb.2007.06.040
  • Leiros, Hanna-Kirsti S.; Pey, AL; Innselset, M; Innselset, Marte; Moe, Elin; Leiros, Ingar; Steen, IH; Martinez, A. 2007. Structure of phenylalanine hydroxylase from Colwellia psychrerythraea 34H, a monomeric cold active enzyme with local flexibility around the active site and high overall stability. Journal of Biological Chemistry. 282. doi: 10.1074/jbc.M610174200
  • Stokke, R; Leiros, Ingar; Karlström, Mikael; Karlstrom, M; Yang, Nannan; Ladenstein, R; Birkeland, NK; Steen, IH. 2007. Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers. Extremophiles. 11. doi: 10.1007/s00792-006-0060-z
  • Stokke, Runar; Birkeland, Nils-Kåre; Steen, Ida Helene. 2007. Thermal stability and biochemical properties of isocitrate dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum. Extremophiles. 11: 397-402. doi: 10.1007/s00792-006-0045-y
  • Stokke, Runar; Madern, Dominique; Fedøy, Anita-Elin; Fedøy, Anita-Elin; Karlsen, Solveig; Birkeland, Nils-Kåre; Steen, Ida Helene. 2007. Biochemical characterization of isocitrate dehydrogenase from Methylococcus capsulatus reveals a unique NAD(+)-dependent homotetrameric enzyme. Archives of Microbiology. 187. doi: 10.1007/s00203-006-0200-y
  • Karlström, Mikael; Steen, Ida Helene; Madern, Dominique; Fedøy, Anita-Elin; Birkeland, Nils-Kåre; Ladenstein, Rudolf. 2006. The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima. The FEBS Journal. 273: 2851-2868. doi: 10.1111/j.1742-4658.2006.05298.x
  • Karlström, Mikael; Stokke, Runar; Steen, Ida Helene; Birkeland, Nils-Kåre; Ladenstein, Rudolf. 2005. Isocitrate dehydrogenase from the hyperthermophile Aeropyrum pernix: X-ray structure analysis of a ternary enzyme-substrate complex and thermal stability. Journal of Molecular Biology. 345: 559-577.
  • Steen, Ida Helene; Madsen, Marit Steine; Birkeland, Nils-Kåre; Lien, Torleiv. 2002. Identification of cofactor discrimination siten in NAD-isocitrate dehydrogenase from Pyrococcus furious. Archives of Microbiology. 178: 297-300.
  • Madern, Dominique; Ebel, C.; Dale, Hege Avsnes; Lien, Torleiv; Steen, Ida Helene; Birkeland, Nils-Kåre; Zaccai, G. 2001. Differences in the oligomeric state of the LDH-like L-MalDH from the hyperthermophilic archaea Methanococcus jannaschii and Archaeoglobus fulgidus. Biochemistry. 40: 10310-10316.
  • Steen, Ida Helene; Hvoslef, Hilde; Birkeland, Nils-Kåre; Lien, Torleiv. 2001. Archaeoglobus fulgidus isocitrate dehydrogenase, malate dehydrogenase and glutamate dehydrogenase. Methods in Enzymology. 331: 13-26.
  • Steen, Ida Helene; Madern, Dominique; Karlstrøm, Mikael; Lien, Torleiv; Ladenstein, R; Birkeland, Nils-Kåre. 2001. Comparison of isocitrate dehydrogenase from three different hyperthermophiles reveals differences in thermostability, cofactor specificity, oligomeric state and phylogenetic affiliation. Journal of Biological Chemistry. 47: 43924-43931.
  • Lien, Torleiv; Madsen, Marit Steine; Steen, Ida Helene; Gjerdevik, Kjersti. 1998. Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oil separation system. International Journal of Systematic Bacteriology. 48: 469-474.
  • Steen, Ida Helene; Madsen, Marit Steine; Birkeland, Nils-Kåre; Lien, Torleiv. 1998. Purification and characterization of a monomeric isocitrate dehydrogenase from the sulfate-reducing bacterium Desulfobacter vibrioformis and demonstration of the presence of a monomeric enzyme in other bacteria. FEMS Microbiology Letters. 160: 469-474.
  • Aalen, Nina; Steen, Ida Helene; Birkeland, Nils-Kåre; Lien, Torleiv. 1997. Purification and properties of an extremely thermostable NADP-specific glutamate dehydrogenase from Archaeoglobus fulgidus. Archives of Microbiology. 168: 536-539.
  • Langelandsvik, Anne Siri; Steen, Ida Helene; Birkeland, Nils-Kåre; Lien, Torleiv. 1997. Properties and primary structure of a thermostable L-malate dehydrogenase from Archaeoglobus fulgidus. Archives of Microbiology. 168: 59-67.
  • Steen, Ida Helene; Lien, Torleiv; Birkeland, Nils-Kåre. 1997. Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, Archaeoglobus fulgidus. Archives of Microbiology. 168: 412-420.
Reports and theses
  • Marques, Ana Filipa Alfaia; Pedersen, Rolf B.; Thorseth, Ingunn Hindenes; Baumberger, Tamara; Roerdink, Desiree; Jørgensen, Steffen Leth; Økland, Ingeborg Elisabet; Olsen, Bernd; Steen, Ida Helene; Hamelin, Cedric; Denny, Alden Ross; Bretel, Patrice. 2015. Arctic Mid-Ocean Ridge, NORMAR/CGB 2015 Test Cruise, R/V G.O.SARS, Senter for Geobiologi. Universitetet i Bergen, Bergen, Norway. 55 pages.
  • Steen, Ida Helene. 1998. Isocitrate dehydrogenase from the sulfate reducers Archaeoglobus fulgidus and Desulfobacter vibrioformis:Properties, Primary structure, and Phylogeny.
Book sections
  • Steen, Ida Helene. 2015. Norske varmekilder i dyphavet. Kap 11, pages 89-95. In:
    • Høyer, Jo. 2015. Årbok for Universitetsmuseet i Bergen 2015. 102 pages. ISBN: 978-82-7887-040-2.

More information in national current research information system (CRIStin)

Current and previous projects:

2016-2019: Virus-X: Viral Metagenomics for innovation Value. H2020. Parther/WP-leader: 496250 EUR

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2015-2018: INMARE: Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea.  H2020. Partner: 220000 EUR More info.

2012-2017: NorZymeD: Enzyme development for Norwegian biomass - mining Norwegian biodiversity for seizing Norwegian oppotunities in the bio-based economy. NFR

2012-2015: Better handling of microbial induced corrosion during operation. VISTA 2.4 mill

2011-2016: Mining of a Norwegian biogoldmine through metagenomics. NFR 8.0 mill

2009-2012: Metagenomics and metaproteomics of deep arctic hydrothermal systems. VISTA. 2.14 mill

2006-2007: Optimalisering av proteomikkverktøy for stuider av fysiologiske prosesser hos mikoorganismer i biogeokjemiske sykluser i ekstreme miljl. Meltzer 460.000 NOK

2001-2002: Thermodynamics and structure of thermostable isocitrate dehydrogenase. Postdoc, Karolinska Institute. NorFa. 

1998-2001: Structural and phylogenetic aspects of isocitrate dehydrogenase. NFR.