Home
Ida Helene Steen's picture

Ida Helene Steen

Professor
  • E-mailIda.Steen@uib.no
  • Phone+47 55 58 83 75
  • Visitor Address
    Thormøhlensgt. 53 A/B
  • Postal Address
    Postboks 7803
    5020 Bergen

The overall theme in my research is to understand the physiology of microorganisms living in deep-sea hydrothermal vent systems by the use of culturing, molecular biology, biochemistry and geochemistry.

Specific questions that I address are:

What is the diversity of energy metabolisms of hydrothermal vents microorganisms?

How do they link the cycling of carbon, nitrogen and sulfur in the system?

Do they have enzymes useful in biotechnology?

Current PhD and master students  (main or co- supervisor)

PhD-student Anders Schouw (UiB, Biogoldmine).Biogenic conversion of higher hydrocarbons to methane - New isolates, functional insights and models.

PhD-student Jan Vander Roost (UiB, CGB). Iron oxidizing bacteria at the Jan Mayen hydrothermal vent field. 

PhD-student Hasan Arsin (UiB, NorZymeD). Industrial application of proteases deriving from extreme environments.

Graduated

PhD Sven Le Moine Bauer. Impact of environmental parameters and dispersal on microbial communities in hydrothermal areas of the Nordic Seas -2017

Msc Andreas Gilje Sjøberg. Physiological characterization of a novel strain within Rhodobacteraceae, isolated from a biofilm sample on a barite chimney at the Loki´s Castle Vent Field. - 2016

PhD Juliane Wissuwa. The Arctic mid-Ocean Ridge Vent Fields - A valuable Resource for Marine Bioprospecting? -2016

MSc Sepideh Mostafavi.Metagenome-based analysis of the functional potential of a marine sediment community dominated by ANME-2c. -2015

MSc Sophie Radeke. Diversity, function and interactions of anaerobic methanotrophs in Loki ́s Castle Vent Field. -2015

MSc Victoria Røyseth. Isolering og fysiologisk karakterisering av Marinitoga sp. stamme 2PyrY55-1ᵀ. -2015

PhD William P. Hocking (UiB). Energy conservation mechnisms in the sulfate respiring  archaeon Archaeoglobus fulgidus VC16-assessed by transcriptome analyses and comparative genomics. -2015

PhD Anita-Elin Fedøy (UiB). Structure-function analysis of isocitrate dehydrogenase subfamily II: Adaptations to hot and cold –2014

PhD Steffen L Jørgensen (UiB, CGB). Linking microbial community structure and geochemistry in deep-sea sediments - 2013

PhD Irene Roalkvam (UiB, VISTA). Diversity, stratification and in situ metabolism of anaerobic methanotrophic archaea in Nyegga cold seeps  - 2012

MSc Elisabeth Machteld Biersma. Microbial diversity and function associated with a black smoker from Loki´s Castle vent field. - 2012

MSc Carina Magnussen. Genekspresjon av karbonmonoksid dehydrogenase/acethyl-CoA syntase komplekset og sat-ORF2-aprBA operonet i Arhcaeoglobus fulgidus. - 2010

MSc Irene Roalkvam. Proteomikkstudier av den sulfatreduserende hypertermofile arkéen Archaeoglobus fulgidus - 2008

MSc Solveig Hoem. Diversitet og utbredelse av sulfatreduserende og svoveloksiderende prokaryoter i hydrothermale miljø.  - 2008

PhD Runar Stokke (UiB). Isocitrate dehydrogenase from extremophiles: Molecular adaptations to high temperatures. - 2007

MSc William P. Hocking. Kinetic characterization revealing a possible NADP substrate inhibition mechanism in isocitrate dehydrogenase of the hyperthermophilic archaeon Aeropyrum pernix. - 2007

Journal articles
  • Wissuwa, Juliane; Le Moine Bauer, Sven; Steen, Ida Helene; Stokke, Runar. 2017. Complete genome sequence of Lutibacter profoundi LP1T isolatet from an Arctic deep-sea hydrothermal vent system. Standards in Genomic Sciences. 12:5: 1-11. doi: 10.1186/s40793-016-0219-x
  • Le Moine Bauer, Sven; Roalkvam, Irene; Steen, Ida Helene; Dahle, Håkon. 2016. Lutibacter profundi sp. nov., isolated from a deep-sea hydrothermal system on the Arctic Mid-Ocean Ridge and emended description of the genus Lutibacter. International Journal of Systematic and Evolutionary Microbiology. 66: 2671-2677. doi: 10.1099/ijsem.0.001105
  • Schouw, Anders; Eide, Tove Leiknes; Stokke, Runar; Pedersen, Rolf B.; Steen, Ida Helene; Bødtker, Gunhild. 2016. Abyssivirga alkaniphila gen. nov., sp. nov., an alkane-degrading, anaerobic bacterium from a deep-sea hydrothermal vent system, and emended descriptions of Natranaerovirga pectinivora and Natranaerovirga hydrolytica. International Journal of Systematic and Evolutionary Microbiology. 66: 1724-1734. doi: 10.1099/ijsem.0.000934
  • Steen, Ida Helene. 2016. Et hav av bakterier er et hav av muligheter. Bergens Tidende.
  • Steen, Ida Helene. 2016. Norske varmekilder i dyphavet - en underfundig verden full av spennende livsformer. Årbok for Universitetsmuseet i Bergen.
  • Steen, Ida Helene; Dahle, Håkon; Stokke, Runar; Roalkvam, Irene; Daae, Frida Lise; Rapp, Hans Tore; Pedersen, Rolf B.; Thorseth, Ingunn Hindenes. 2016. Novel barite chimneys at the Loki´s Castle vent field shed light on key factors shaping microbial communities and functions in hydrothermal systems. Frontiers in Microbiology. 6:1510. doi: 10.3389/fmicb.2015.01510
  • Steinsbu, Bjørn Olav; Røyseth, Victoria; Thorseth, Ingunn Hindenes; Steen, Ida Helene. 2016. Marinitoga arctica sp. nov., a thermophilic, anaerobic heterotroph isolated from the Soria Moria vent field on the Arctic Mid-Ocean Ridge. International Journal of Systematic and Evolutionary Microbiology. doi: 10.1099/ijsem.0.001472
  • Tvedt, Morten Walløe; Eijsink, Vincentius Gerardus Henricus; Steen, Ida Helene; Strand, Roger; Rosendal, Guri Kristin. 2016. The missing link in ABS: The relationship between resource and product. Environmental Policy and Law. 46: 227-237.
  • Wissuwa, Juliane; Stokke, Runar; Fedøy, Anita-Elin; Lian, Kjersti; Smalås, Arne O.; Steen, Ida Helene. 2016. Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site. Standards in Genomic Sciences. 11:16: 1-12. doi: 10.1186/s40793-016-0137-y
  • Dahle, Håkon; Økland, Ingeborg Elisabet; Thorseth, Ingunn Hindenes; Pedersen, Rolf B.; Steen, Ida Helene. 2015. Energy landscapes shape microbial communities in hydrothermal systems on the Arctic Mid-Ocean Ridge. The ISME Journal. 9: 1593-1606. doi: 10.1038/ismej.2014.247
  • Hocking, William Peter; Roalkvam, Irene; Magnussen, Carina; Stokke, Runar; Steen, Ida Helene. 2015. Assessment of the carbon monoxide metabolism of the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus VC-16 by comparative transcriptome analyses. Archaea. 2015:235384. 12 pages. doi: 10.1155/2015/235384
  • Roalkvam, Irene; Bredy, Florian; Baumberger, Tamara; Pedersen, Rolf B.; Steen, Ida Helene. 2015. Hypnocyclicus thermotrophus gen. Nov., sp. nov. isolated from a microbial mat in a hydrothermal vent field. International Journal of Systematic and Evolutionary Microbiology. 65: 4521-4525. doi: 10.1099/ijsem.0.000606
  • Roalkvam, Irene; Drønen, Karine; Stokke, Runar; Daae, Frida Lise; Dahle, Håkon; Steen, Ida Helene. 2015. Physiological and genomic characterization of Arcobacter anaerophilus IR-1 reveals new metabolic features in Epsilonproteobacteria. Frontiers in Microbiology. 6. doi: 10.3389/fmicb.2015.00987
  • Stokke, Runar; Dahle, Håkon; Roalkvam, Irene; Wissuwa, Juliane; Daae, Frida Lise; Tooming-Klunderud, Ave; Thorseth, Ingunn Hindenes; Pedersen, Rolf B.; Steen, Ida Helene. 2015. Functional interactions among filamentous Epsilonproteobacteria and Bacteroidetes in a deep-sea hydrothermal vent biofilm. Environmental Microbiology. 17: 4063-4077. doi: 10.1111/1462-2920.12970
  • Drønen, Karine; Roalkvam, Irene; Beeder, Janiche; Torsvik, Terje; Steen, Ida Helene; Skauge, Arne; Liengen, Turid. 2014. Modeling of heavy nitrate corrosion in anaerobe aquifer injection water biofilm: A case study in a flow rig. Environmental Science and Technology. 48: 8627-8635. doi: 10.1021/es500839u
  • Hocking, William Peter; Stokke, Runar; Roalkvam, Irene; Steen, Ida Helene. 2014. Identification of key components in the energy metabolism of Archaeoglobus fulgidus by transcriptome analyses. Frontiers in Microbiology. 5:95. doi: 10.3389/fmicb.2014.00095
  • Urich, Tim; Lanzén, Anders; Stokke, Runar; Pedersen, Rolf B.; Bayer, Christoph; Thorseth, Ingunn Hindenes; Schleper, Christa Maria; Steen, Ida Helene; Øvreås, Lise. 2014. Microbial community structure and functioning in marine sediments associated with diffuse hydrothermal venting assessed by integrated meta-omics. Environmental Microbiology. 16: 2699-2710. doi: 10.1111/1462-2920.12283
  • Dahle, Håkon; Roalkvam, Irene; Pedersen, Rolf B.; Thorseth, Ingunn Hindenes; Steen, Ida Helene. 2013. The versatile in situ gene expression of an Epsilonproteobacteria-dominated biofilm from a hydrothermal chimney. Environmental Microbiology Reports. 5: 282-290. doi: 10.1111/1758-2229.12016
  • Stokke, Runar; Hocking, William Peter; Steinsbu, Bjørn Olav; Steen, Ida Helene. 2013. Complete genome sequence of the thermophilic and facultatively chemolithoautotrophic sulfate reducer Archaeoglobus sulfaticallidus PM70-1T. Genome Announcements.
  • Jørgensen, Steffen Leth; Hannisdal, Bjarte; Lanzén, Anders; Baumberger, Tamara; Flesland, Kristin; Fonseca, Rita; Øvreås, Lise; Steen, Ida Helene; Thorseth, Ingunn Hindenes; Pedersen, Rolf B.; Schleper, Christa Maria. 2012. Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge. Proceedings of the National Academy of Sciences of the United States of America. 109: E2846-E2855. doi: 10.1073/pnas.1207574109
  • Leiros, Hanna-Kirsti S.; Fedøy, Anita-Elin; Leiros, Ingar; Steen, Ida Helene. 2012. The complex structures of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila support a new active site locking mechanism. FEBS Open Bio.
  • Ray, Jessica Louise; Dondrup, Michael; Modha, Sejal; Steen, Ida Helene; Sandaa, Ruth-Anne; Clokie, Martha. 2012. Finding a needle in the virus metagenome haystack - micro-metagenome analysis captures a snapshot of the diversity of a bacteriophage armoire. PLoS ONE. 7. 13 pages. doi: 10.1371/journal.pone.0034238
  • Roalkvam, Irene; Dahle, Håkon; Chen, Yifeng; Jørgensen, Steffen Leth; Haflidason, Haflidi; Steen, Ida Helene. 2012. Fine-scale community structure analysis of ANME in Nyegga sediments with high and low methane flux. Frontiers in Microbiology. 3: 1-13. doi: 10.3389/fmicb.2012.00216
  • Stokke, Runar; Roalkvam, Irene; Lanzén, Anders; Haflidason, Haflidi; Steen, Ida Helene. 2012. Integrated metagenomic and metaproteomic analyses of an ANME-1-dominated community in marine cold seep sediments. Environmental Microbiology. 14: 1333-1346. doi: 10.1111/j.1462-2920.2012.02716.x
  • Roalkvam, Irene; Jørgensen, Steffen Leth; Chen, Yifeng; Stokke, Runar; Dahle, Håkon; Hocking, William Peter; Lanzén, Anders; Haflidason, Haflidi; Steen, Ida Helene. 2011. New insight into stratification of anaerobic methanotrophs in cold seep sediments. FEMS Microbiology Ecology. 78: 233-243. doi: 10.1111/j.1574-6941.2011.01153.x
  • Karlström, Mikael; Chiaraluce, Roberta; Giangiacomo, Laura; Steen, Ida Helene; Birkeland, Nils-Kåre; Ladenstein, Rudolf; Consalvi, Valerio. 2010. Thermodynamic and kinetic stability of a large multi-domain enzyme from the hyperthermophile Aeropyrum pernix. Extremophiles. 14: 213-223. doi: 10.1007/s00792-009-0300-0
  • Siltberg-Liberles, Jessica; Steen, Ida Helene; Svebak, Randi Myrseth; Martinez, Aurora. 2008. The phylogeny of the aromatic amino acid hydroxylases revisited by characterizing phenylalanine hydroxylase from Dictyostelium discoideum. Gene. 427: 86-92. doi: 10.1016/j.gene.2008.09.005
  • Fedøy, Anita-Elin; Fedoy, AE; Yang, Nannan; Martinez, A; Leiros, Hanna-Kirsti S.; Steen, IH. 2007. Structural and functional properties of isocitrate dehydrogenase from the psychrophilic bacterium Desulfotalea psychrophila reveal a cold-active enzyme with an unusual high thermal stability. Journal of Molecular Biology. 372: 130-149. doi: 10.1016/j.jmb.2007.06.040
  • Leiros, Hanna-Kirsti S.; Pey, AL; Innselset, M; Innselset, Marte; Moe, Elin; Leiros, Ingar; Steen, IH; Martinez, A. 2007. Structure of phenylalanine hydroxylase from Colwellia psychrerythraea 34H, a monomeric cold active enzyme with local flexibility around the active site and high overall stability. Journal of Biological Chemistry. 282. doi: 10.1074/jbc.M610174200
  • Stokke, R; Leiros, Ingar; Karlström, Mikael; Karlstrom, M; Yang, Nannan; Ladenstein, R; Birkeland, NK; Steen, IH. 2007. Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers. Extremophiles. 11. doi: 10.1007/s00792-006-0060-z
  • Stokke, Runar; Birkeland, Nils-Kåre; Steen, Ida Helene. 2007. Thermal stability and biochemical properties of isocitrate dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum. Extremophiles. 11: 397-402. doi: 10.1007/s00792-006-0045-y
  • Stokke, Runar; Madern, Dominique; Fedøy, Anita-Elin; Fedøy, Anita-Elin; Karlsen, Solveig; Birkeland, Nils-Kåre; Steen, Ida Helene. 2007. Biochemical characterization of isocitrate dehydrogenase from Methylococcus capsulatus reveals a unique NAD(+)-dependent homotetrameric enzyme. Archives of Microbiology. 187. doi: 10.1007/s00203-006-0200-y
  • Karlström, Mikael; Steen, Ida Helene; Madern, Dominique; Fedøy, Anita-Elin; Birkeland, Nils-Kåre; Ladenstein, Rudolf. 2006. The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima. The FEBS Journal. 273: 2851-2868. doi: 10.1111/j.1742-4658.2006.05298.x
  • Karlström, Mikael; Stokke, Runar; Steen, Ida Helene; Birkeland, Nils-Kåre; Ladenstein, Rudolf. 2005. Isocitrate dehydrogenase from the hyperthermophile Aeropyrum pernix: X-ray structure analysis of a ternary enzyme-substrate complex and thermal stability. Journal of Molecular Biology. 345: 559-577.
  • Steen, Ida Helene; Madsen, Marit Steine; Birkeland, Nils-Kåre; Lien, Torleiv. 2002. Identification of cofactor discrimination siten in NAD-isocitrate dehydrogenase from Pyrococcus furious. Archives of Microbiology. 178: 297-300.
  • Madern, Dominique; Ebel, C.; Dale, Hege Avsnes; Lien, Torleiv; Steen, Ida Helene; Birkeland, Nils-Kåre; Zaccai, G. 2001. Differences in the oligomeric state of the LDH-like L-MalDH from the hyperthermophilic archaea Methanococcus jannaschii and Archaeoglobus fulgidus. Biochemistry. 40: 10310-10316.
  • Steen, Ida Helene; Hvoslef, Hilde; Birkeland, Nils-Kåre; Lien, Torleiv. 2001. Archaeoglobus fulgidus isocitrate dehydrogenase, malate dehydrogenase and glutamate dehydrogenase. Methods in Enzymology. 331: 13-26.
  • Steen, Ida Helene; Madern, Dominique; Karlstrøm, Mikael; Lien, Torleiv; Ladenstein, R; Birkeland, Nils-Kåre. 2001. Comparison of isocitrate dehydrogenase from three different hyperthermophiles reveals differences in thermostability, cofactor specificity, oligomeric state and phylogenetic affiliation. Journal of Biological Chemistry. 47: 43924-43931.
  • Lien, Torleiv; Madsen, Marit Steine; Steen, Ida Helene; Gjerdevik, Kjersti. 1998. Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oil separation system. International Journal of Systematic Bacteriology. 48: 469-474.
  • Steen, Ida Helene; Madsen, Marit Steine; Birkeland, Nils-Kåre; Lien, Torleiv. 1998. Purification and characterization of a monomeric isocitrate dehydrogenase from the sulfate-reducing bacterium Desulfobacter vibrioformis and demonstration of the presence of a monomeric enzyme in other bacteria. FEMS Microbiology Letters. 160: 469-474.
  • Aalen, Nina; Steen, Ida Helene; Birkeland, Nils-Kåre; Lien, Torleiv. 1997. Purification and properties of an extremely thermostable NADP-specific glutamate dehydrogenase from Archaeoglobus fulgidus. Archives of Microbiology. 168: 536-539.
  • Langelandsvik, Anne Siri; Steen, Ida Helene; Birkeland, Nils-Kåre; Lien, Torleiv. 1997. Properties and primary structure of a thermostable L-malate dehydrogenase from Archaeoglobus fulgidus. Archives of Microbiology. 168: 59-67.
  • Steen, Ida Helene; Lien, Torleiv; Birkeland, Nils-Kåre. 1997. Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, Archaeoglobus fulgidus. Archives of Microbiology. 168: 412-420.
Reports and theses
  • Marques, Ana Filipa Alfaia; Pedersen, Rolf B.; Thorseth, Ingunn Hindenes; Baumberger, Tamara; Roerdink, Desiree; Jørgensen, Steffen Leth; Økland, Ingeborg Elisabet; Olsen, Bernd; Steen, Ida Helene; Hamelin, Cedric; Denny, Alden Ross; Bretel, Patrice. 2015. Arctic Mid-Ocean Ridge, NORMAR/CGB 2015 Test Cruise, R/V G.O.SARS, Senter for Geobiologi. Universitetet i Bergen, Bergen, Norway. 55 pages.
  • Steen, Ida Helene. 1998. Isocitrate dehydrogenase from the sulfate reducers Archaeoglobus fulgidus and Desulfobacter vibrioformis:Properties, Primary structure, and Phylogeny.
Book sections
  • Steen, Ida Helene. 2015. Norske varmekilder i dyphavet. Kap 11, pages 89-95. In:
    • Høyer, Jo. 2015. Årbok for Universitetsmuseet i Bergen 2015. 102 pages. ISBN: 978-82-7887-040-2.

More information in national current research information system (CRIStin)

Ursula Düren. Syntrophic conversion of protein-rich compounds to methane - fall 2017 

Francesca Vulcano. Syntrophic conversion of hydrocarbons to methane - spring 2017

Florian Bredy. Characterization of Hypnocyclicus thermotrophus gen. nov. sp. nov., a sugar fermenting bacterium from the Seven Sisters hydrothermal vent field - 2014/2015

Current and previous projects:

2016-2019: Virus-X: Viral Metagenomics for innovation Value. H2020. Parther/WP-leader: 496250 EUR

96

2015-2018: INMARE: Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea.  H2020. Partner: 220000 EUR More info.

2012-2017: NorZymeD: Enzyme development for Norwegian biomass - mining Norwegian biodiversity for seizing Norwegian oppotunities in the bio-based economy. NFR

2012-2015: Better handling of microbial induced corrosion during operation. VISTA 2.4 mill

2011-2016: Mining of a Norwegian biogoldmine through metagenomics. NFR 8.0 mill

2009-2012: Metagenomics and metaproteomics of deep arctic hydrothermal systems. VISTA. 2.14 mill

2006-2007: Optimalisering av proteomikkverktøy for stuider av fysiologiske prosesser hos mikoorganismer i biogeokjemiske sykluser i ekstreme miljl. Meltzer 460.000 NOK

2001-2002: Thermodynamics and structure of thermostable isocitrate dehydrogenase. Postdoc, Karolinska Institute. NorFa. 

1998-2001: Structural and phylogenetic aspects of isocitrate dehydrogenase. NFR.