Fluorescence- and electron microscopy
Is used to identify changes in protein levels, localization and modification which are associated with cellular processes. It is also used to identify the chemical structure of unknown compounds.
DNA and RNA analysis (PCR, q-PCR, microarray, chromatin immuno-precipitation) as well as in vitro studies on purified proteins (immuno- blotting, kinetic analysis, spectroscopy, surface plasmon resonance) are extensively applied.
We use ODE and stochastic simulations to assist our understanding of cellular signaling and how the kinetic properties of proteins determine the dynamics of cellular processes.