Rickard Sandberg: "Analyses of allelic gene expression in single cells"
Single-cell sequencing reveals far more mono-allelic gene expression than previously thought; - seemingly random, and dynamic over time.
Hovedinnhold
"Analyses of allelic gene expression in single cells"
by Professor Rickard Sandberg, Karolinska Institute, Stockholm
Venue: Department of Molecular Biology Seminar room: "The N-terminal",
High Technology Centre, Bergen, Thursday Nov 10th at 11:00
Assessing gene expression in individual cells holds promise to reveal the extent, function and origins of cell-to-cell variability. To this end, my lab has been developing single-cell RNA-sequencing methods (e.g. Smart-seq2), and using the full-length feature of Smart-seq2 together with the detection of heterozygous SNPs we have investigated gene expression at allelic resolution. Analyses across thousands of individual cells revealed fundamental features of allelic expression from autosomes and sex chromosomes. In early embryonic development, female mouse and human embryos achieve dosage compensation of X-chromosome RNA levels using surprisingly different strategies. In mouse, the paternal X chromosome becomes inactivated in 4-cell embryos whereas dosage compensation in human embryos proceeds with a dampening of expression from both X chromosomes. Single-cell analyses of allelic expression of autosomal genes revealed that for a substantial fraction of genes in a cell, the transcribed RNA is derived from only one parental allele. The expression of alleles in a cell was seemingly random, and dynamic over time, even within cells of clonal origins. The allelic resolution was also used to examine other forms of transcriptional and post-transcriptional regulation of protein coding and long non-coding RNAs, demonstrating the power and general utility of allelic resolution single-cell RNA-sequencing to study gene regulation.
Later in the evening, at 16:15 in Egget, Studentsenteret, Professor Sandberg gives the inaugural Kjell Kleppe Lecture jointly with the Horizons Lecture Series.
Just before this lecture, at 15:15, also in Egget, Studentsenteret, MBI will honor the memory and legacy of Professor Kjell Kleppe with the event: Professor Kjell Kleppe (1934-1988) - Biochemist, genetic engineer and pioneer, featuring professors Lars Haarr, Johan R. Lillehaug, and Dag E. Helland
Everyone is welcome.