Harald G Wiker
professor
Gades institutt
Infeksjon
Stilling: professor
Telefon: 55 97 46 50
E-post: Harald.Wiker@gades.uib.no
Besøksadresse: Laboratoriebygget vest, 5. etg. - Helse-Bergen
Clinical and experimental microbiology and immunology with an emphasis on characterization of mycobacteria using proteomic methods
MD, pHD, Medical speciality in Immunology
1. Beyene, D., Bergval, I., Hailu, E., Ashenafi, S., Yamuah, L.K., Aseffa, A., Wiker, H.G., Engers, H.D., Klatser, P.R. and Sviland, L. Identification and genotyping of the etiological agent of tuberculous lymphadenitis in Ethiopia. J Infect Dev Ctries 2009; 3(6):412-419.
2. Pathak, S., De Souza G.A., Salte, T., Wiker H.G. and Åsjø, B. HIV Induces both a Down-Regulation of IRAK-4 that Impairs TLR Signalling and an Up-regulation of the Antibiotic Peptide Dermcidin in Monocytic Cells. Scand J Immunol. 2009; 70:264-276.
3. Ellertsen, L.K., Storla, D.G., Diep, L.M., Brokstad, K.A., Wiker H.G., and Hetland G. Allergy markers in active pulmonary tuberculosis at time of diagnosis and following chemotherapy. BMC Infect Dis 2009; 9: 100.
4. DeSouza, G.A., and Wiker, H.G. The impact of proteomic advances on bacterial gene annotation. Current Proteomics 2009; 6: 84-92.
5. Leversen, N.A., DeSouza, G.A., Målen, H., Prasad, S., Jonassen, I. and Wiker, H.G. Evaluation of signal peptide prediction algorithms for identification of mycobacterial signal peptides using sequence data from proteomic methods. Microbiology 2009; 155: 2375-83.
6. DeSouza G.A. Søfteland, T., Kohler, C., Thiede, B. and Wiker H.G. Validating divergent ORF annotation of the Mycobacterium leprae genome through a full translation dataset and peptide identification by tandem mass spectrometry. Proteomics 2009; 9: 3233-43.
7. Wiker, H.G. MPB70 and MPB83 – major antigens of Mycobacterium bovis. Scand J Immunol. 69:492-9.
8. Olsen I. and Wiker HG. Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support. Methods Mol Biol. 2009;536:35-8.
2008
9. Beyene, D., Ashenafi, S., Yamuah, L.K., Aseffa, A., Wiker, H.G., Engers, H.D., Klatser, P.R. and Sviland, L. Clinical diagnosis of tuberculous lymphadenitis in Ethiopia: Correlation with culture, histology and HIV status. Int J Tuberc Lung Dis. 2008; 12: 1030-6.
10. Baba, K., A. M. Dyrhol-Riise, L. Sviland, N. Langeland, A. A. Hoosen, H. G. Wiker, and T. Mustafa. Rapid and Specific Diagnosis of Tuberculous Pleuritis With Immunohistochemistry by Detecting Mycobacterium tuberculosis Complex Specific Antigen MPT64 in Patients From a HIV Endemic Area. Appl. Immunohistochem. Mol. Morphol. 2008; 16: 554-61.
11. De Souza G.A., Målen, H., Søfteland, T., Sælensminde, G., Prasad, S., Jonassen, I. and Wiker H.G. High accuracy mass spectrometry analysis as a tool to verify and improve gene annotation using Mycobacterium tuberculosis as an example BMC Genomics. 2008; 9:316
12. Målen, H., Berven, F.S., Søfteland, T., Arntzen, M.Ø., D’Santos, C.S. and Wiker, H.G. Membrane- and membrane associated proteins in Triton X-114 extracts of Mycobacterium bovis BCG identified using a combination of gel-based and gel-free fractionation strategies. Proteomics. 2008; 8: 1859-1870
13. Mustafa, T., Wiker, H.G., Mørkve, O. and Sviland, L. Differential expression of mycobacterial antigen MPT64, apoptosis and inflammatory markers in multinucleated giant cells and epithelioid cells in granulomas caused by Mycobacterium tuberculosis. Virchows Archives; 2008; 452:449-456
14. Målen, H., Søfteland, T., Wiker, H.G. Antigen analysis of Mycobacterium tuberculosis H37Rv culture filtrate proteins, Scand J Immunol 2008; 67: 245-252
2007
15. Purohit, M.R., Mustafa, T., Wiker, H.G., Mørkve,O., Sviland, L. Immunohistochemical diagnosis of abdominal and lymph node tuberculosis by detecting Mycobacterium tuberculosis complex specific antigen MPT64. Diagn. Pathol. 2007; 2:36
16. Rydjord, B., Eduard, W., Stensby, B., Sandven, P., Michaelsen, T., and Wiker, H. G. Antibody Response to Long-term and High-dose Mould-exposed Sawmill Workers Scand J. Immunol. 2007; 66: 711-718
17. Mustafa, T., Wiker, H.G., Mørkve, O. and Sviland, L. Reduced apoptosis and increased inflammatory cytokines in granulomas caused by tuberculous as compared to non-tuberculous mycobacteria-role of MPT64 antigen in apoptosis and immune response. Clin Exp Immunol. 2007; 150: 105-113
18. Abebe, F., Holm-Hansen, C., Wiker, H.G. and Bjune, G. Progress in serodiagnosis of Mycobacterium tuberculosis infection. Scand J Immunol. 2007; 66:176
19. Rydjord, B., Namork, E., Wiker, H.G., Hetland, G. Quantification and Characterisation of IgG-binding to Mould Spores by Flow Cytometry and Scanning Electron Microscopy. J Immunol Methods, 2007 323: 123-31
20. Målen, H., Berven, F. Fladmark, K.E. and Wiker H.G. Comprehensive Analysis of Exported Proteins from Mycobacterium tuberculosis H37Rv. Proteomics. 2007; 7:1702-18
2006
21. Shrivastava, R., Das, D.R., Wiker, H.G., Das, A.K. Modelling and docking of the proteins of a unique two-component system from Mycobacterium tuberculosis and the mechanism of phosphotransfer. Biochem Biophys Res Commun. 2006; 344:1327-33.
22. Al-Attiyah, R., Madi, N.M., El-Shamy, A.S.M., Wiker, H.G., Andersen, P., Mustafa, A.S. Cytokine Profiles in Tuberculosis Patients and Healthy Subjects in Response to Complex and Single Antigens of Mycobacterium tuberculosis. FEMS Immunology and Medicial Microbiology, 2006; 47:254-61.
23. Wiker, H. G., Mustafa, T., Målen, H., Riise, A. M. D.. Novel vaccine approaches to prevent tuberculosis. Review article. Scandinavian Journal of Immunology. 2006; 64:243-50.
24. Mustafa, T., Wiker, H.G., Mfinanga, S.G.M., Mørkve, O. and Sviland, L. Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis. Modern Pathology, 2006;19:1606-14
25. Wiker, H.G. Mykobakterieinfeksjoner. Naturen 2006;130:114-20. (in norwegian)
26. Wiker, H.G. og Bjune, G. Vaksiner mot tuberkulose. Tidsskrift for den norske lægeforening. 2006; 126:2678-81. (in norwegian)
2005
27. Madhurantakam, C., Erappa, R., Mazumdar, P.A., Saha, B., Mitra, D., Wiker, H.G., Sankaranarayanan, R.,and Das, A.K. Crystal Structure of Low Molecular Weight Protein Tyrosine Phosphatase (MPtpA) from Mycobacterium tuberculosis at 1.9 Å resolution. J Bacteriol. 2005; 87(6):2175-81.
28. Charlet D., Mostowy, S., David Alexander, D., Sit, L., Wiker, H.G., Behr, M.A. Reduced expression of antigenic proteins MPB70 and MPB83 in Mycobacterium bovis BCG strains due to a start codon mutation in sigK Mol Microbiol 2005; 56:1302-13
29. Rydjord, B., Hetland, G. and Wiker, H.G. Immunoglobulin G antibodies against environmental moulds in a Norwegian healthy population shows a bimodal distribution for Aspergillus versicolor. Scand J Immunol. 2005 Sep;62(3):281-8.
30. Ellertsen, L.K., Wiker, H.G., Egeberg, N.T., Hetland, G. Allergic sensitization in tuberculosis and leprosy patients. Int Arch Allergy Immunol 2005;138:217-224
31. Mitra, D., Saha, B., Das, D., Wiker, H.G. Das, A.K. Correlating sequential homology of Mce1A, Mce2A, Mce3A and Mce4A with their possible functions in mammalian cell entry of Mycobacterium tuberculosis by homology modeling. Tuberculosis. 2005;85:337-45
2004
32. Harboe M, Das AK, Mitra D, Ulvund G, Ahmad S, Harkness RE, Das D, Mustafa AS, Wiker HG. Immunodominant B-cell epitope in the Mce1A mammalian cell entry protein of Mycobacterium tuberculosis cross-reacting with glutathione S-transferase. Scand J Immunol. 2004 Feb;59(2):190-7.
33. Wiker, H. G. Unmatched sequences in public databases - exemplified by tuberculin-active protein.
Scand J Immunol. 2004 Jun;59(6):607-8.
34. Faeste CK, Løvik M, Wiker HG, Egaas E. A Case of Peanut Cross-Allergy to Lupine Flour in a Hot Dog Bread. Int Arch Allergy Immunol 2004; (135): 36-9.
35. Instanes C, Ormstad H, Rydjord B, Wiker HG, Hetland G. Mould extracts increase the allergic response to ovalbumin in mice. Clin Exp Allergy 2004; 34:1634–164