Kari Espolin Fladmarks bilde
Kari Espolin
Fladmark
Professor
Besøksadresse: 
HIB - Thormøhlensgt. 55
5020 Bergen
Postadresse: 
Postboks 7803
5020 Bergen
Telefon: 
+47 55 58 45 14
Last ned visittkort

 

Vår forskningsgruppe prøver å forstå hvordan stress fører til celledød og hvilke mekansimer cellene har for å beskytte seg selv og omliggende celler. Vi bruker zebrafisk som in vivo modell for å studere signaler som fører til celledød og beskyttelse. Zebrafisk er blitt en svært populær modell for menneskelig sykdommer. Zebrafiskens utvikling og organer er like våre og sykdomsrelaterte menneskelige gener finnes som funksjonelle gener også i zebrafisk.

Tidsskriftartikler
  • Kamil, Atif; Raae, Arnt Johan; Fjelldal, Per Gunnar; Koppang, Erling Olaf; Fladmark, Kari Espolin; Hordvik, Ivar. 2013. Comparative analysis of IgM sub-variants in salmonid fish and identification of a residue in mu 3 which is essential for MAb4C10 reactivity. Fish and Shellfish Immunology. 34: 667-672. doi: 10.1016/j.fsi.2012.12.006
  • Opsahl, Jill Anette; Ljostveit, Sonja; Saunders, Threrese Solstad; Risa, Kristin; Roepstorff, Peter; Fladmark, Kari Espolin. 2013. Identification of dynamic changes in proteins associated with the cellular cytoskeleton after exposure to okadaic acid. Marine Drugs. 11: 1763-1782. doi: 10.3390/md11061763
  • Hjørnevik, Linda Veka; Fismen, Lise; Young, Fiona M; Solstad, Terese; Fladmark, Kari Espolin. 2012. Nodularin exposure induces SOD1 phosphorylation and disrupts SOD1 co-localization with actin filaments. Toxins. 4: 1482-1499. doi: 10.3390/toxins4121482
  • Oveland, Eystein; Wergeland, Line; Hovland, Randi; Lorens, James; Gjertsen, Bjørn Tore; Fladmark, Kari Espolin. 2012. Ectopic expression of Flt3 kinase inhibits proliferation and promotes cell death in different human cancer cell lines. Cell Biology and Toxicology. 28: 201-212. doi: 10.1007/s10565-012-9216-z
  • de Vareilles, Mahut; Conceição, Luis E.C.; Gomez Requeni, Pedro; Kousoulaki, Katerina; Richard, Nadège; Rodrigues, Pedro M.; Fladmark, Kari Espolin; Rønnestad, Ivar. 2012. Dietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a comparative 2D-DIGE study. Marine Biotechnology. 14: 643-654. doi: 10.1007/s10126-012-9462-3
  • de Vareilles, Mahut; Richard, Nadège; Gavaia, Paulo J.; Silva, Tomé S.; Cordeiro, Odete; Guerreiro, Inês; Yúfera, Manuel; Batista, Irineu; Pires, Carla; Pousao-Ferreira, Pedro; Rodrigues, Pedro M.; Rønnestad, Ivar; Fladmark, Kari Espolin; Conceição, Luís E.C. 2012. Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae. Journal of Applied Ichthyology. 28: 477-487. doi: 10.1111/j.1439-0426.2012.01986.x
  • Opsahl, Jill Anette; Hjørnevik, Linda Veka; Bull, Vibeke Hervik; Fismen, Lise; Frøyset, Ann Kristin; Gromyko, Darina; Solstad, Therese; Fladmark, Kari Espolin. 2010. Increased interaction between DJ-1 and the Mi-2/nucleosome remodelling and deacetylase complex during cellular stress. Proteomics. 10: 1494-1504. doi: 10.1002/pmic.200900586
  • Herfindal, Lars; Kasprzykowski, Franciszek; Schwede, Frank; Lankiewicz, Leszek; Fladmark, Kari Espolin; Lukomska, Joanna; Wahlsten, Matti; Sivonen, Kaarina; Grzonka, Zbigniew; Jastorff, Bernd; Doskeland, Stein Ove. 2009. Acyloxymethyl esterification of Nodularin-R and Microcystin-LA produces inactive protoxins that become reactivated and produce apoptosis inside intact cells. Journal of Medicinal Chemistry. 52: 5758-5762. doi: 10.1021/jm900502e
  • Kellmann, Ralf; Schaffner, Carlos António Markus; Grønset, Toril Anne; Satake, Masayuki; Ziegler, Mathias; Fladmark, Kari Espolin. 2009. Proteomic response of human neuroblastoma cells to azaspiracid-1. Journal of Proteomics. 72: 695-707. doi: 10.1016/j.jprot.2009.02.008
  • Oveland, Eystein; Gjertsen, Bjørn Tore; Wergeland, Line; Selheim, Frode; Fladmark, Kari Espolin; Hovland, Randi. 2009. Ligand-induced Flt3-downregulation modulates cell death associated proteins and enhances chemosensitivity to idarubicin in THP-1 acute myeloid leukemia cells. Leukemia research : a Forum for Studies on Leukemia and Normal Hemopoiesis. 33: 276-287. doi: 10.1016/j.leukres.2008.06.029
  • Solstad, Therese; Bull, Vibeke Hervik; Breivik, Lars Ertesvåg; Fladmark, Kari Espolin. 2008. Identification of protein interaction partners by immunoprecipitation: Possible pitfalls and false positives. Experimental and Clinical Hepatology. 4: 16-22.
  • Solstad, Therese; Fismen, Lise; Garberg, Hilde Kristin; Fladmark, Kari Espolin. 2008. Identification of a novel phosphorylation site of acyl-CoA binding protein (ACBP) in nodularin-induced apoptotic hepatocytes. Experimental Cell Research. 314: 2141-2149. doi: 10.1016/j.yexcr.2008.03.014
  • Målen, Hiwa; Berven, Frode; Fladmark, Kari Espolin; Wiker, Harald G. 2007. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics. 7: 1702-1718. doi: 10.1002/pmic.200600853
  • Brøgger, Jan Christian; Fladmark, Kari Espolin. 2006. Proteomic tissue imaging for rapid brain biomarker discovery. European Journal of Neurology. 13: 42-42.
  • Krakstad, Camilla; Herfindal, Lars; Gjertsen, Bjørn Tore; Bøe, Roald; Vintermyr, Olav Karsten; Fladmark, Kari Espolin; Doskeland, Stein Ove. 2006. CaM-kinasell-dependent commitment to microcystin-induced apoptosis is coupled to cell budding, but not to shrinkage or chromatin hypercondensation. Cell Death and Differentiation. 13: 1191-1202. doi: 10.1038/sj.cdd.4401798
  • Oveland, Eystein; Fladmark, Kari Espolin; Wergeland, Line; Gjertsen, Bjørn Tore; Hovland, Randi. 2006. Proteomics approaches to elucidate oncogenic tyrosine kinase signaling in myeloid malignancies. Current Pharmaceutical Biotechnology. 7: 185-198. doi: 10.2174/138920106777549696
  • Solstad, Therese; Fladmark, Kari Espolin. 2006. Algal toxins as guidance to identify phosphoproteins with key roles in apoptotic cell death. Current Pharmaceutical Biotechnology. 7: 209-215. doi: 10.2174/138920106777549704
  • Gausdal, Gro; Gjertsen, Bjørn Tore; Fladmark, Kari Espolin; Demol, H.; Vandekerckhove, Joel; Døskeland, Stein Ove. 2004. Caspase-dependent, geldanamycin-enhanced cleavage of co-chaperone p23 in leukemic apoptosis. Leukemia. 18: 1989-1996.
  • Fladmark, Kari Espolin. 2003. PROBE-nasjonal plattform i proteomikk. NBS-nytt. 2: 23-24.
  • Grav, Hans J; Tronstad, Karl J.; Gudbrandsen, Oddrun A.; Berge, Kjetil; Fladmark, Kari E.; Martinsen, Tom C.; Waldum, Helge; Wergedahl, Hege; Berge, Rolf K. 2003. Changed energy state and increased mitochondrial beta-oxidation rate in liver of rats associated with lowered proton electrochemical potential and stimulated uncoupling protein 2 (UCP-2) expression. Journal of Biological Chemistry. 278: 30525-30533.
  • Grav, Hans J; Tronstad, Karl Johan; Gudbrandsen, Oddrun; Berge, Kjetil; Fladmark, Kari Espolin; Martinsen, Tom C; Waldum, Helge; Wergedahl, Hege; Berge, Rolf Kristian. 2003. Changed Energy State and Increased Mitochondrial B-Oxidation Rate in Liver of Rats Associated with Lowered Proton Electrochemical Potential and Stimulated Uncoupling Protein 2 (UCP-2) Expression. Journal of Biological Chemistry. 278: 30525-30533.
  • Fladmark, Kari Espolin; Brustugun, Odd Terje; Mellgren, Gunnar; Krakstad, Camilla; Bøe, Roald; Vintermyr, Olav Karsten; Schulman, Howard; Døskeland, Stein Ove. 2002. Ca2+/calmodulin dependent protein kinase II is required for microcystin-induced apoptosis. Journal of Biological Chemistry. 277: 2804-2811.
  • Fladmark, Kari Espolin; Birkeland, Magne. 2001. Biologisk baserte blåskjelltester (BBB). Fiskehelse. 2. 26-33.
  • Mankiewicz, J; Tarczynska, M; Fladmark, Kari Espolin; Døskeland, Stein Ove; Walter, Z; Zalewski, M. 2001. Apoptotic effect of cyanobacterial extract on rat hepatocytes and human lymphocytes. Environmental Toxicology. 16: 225-233.
  • Mankiewitcz, J; Walter, Z; Tarczynska, M; Zalewski, M; Fladmark, Kari Espolin; Døskeland, Stein Ove. 2001. Apoptotic effect of cyanobacterial blooms collected from Polish water reservoirs. International Journal of Occupational Medicine and Environmental Health. 13: 335-344.
  • Serres, Margrethe Hauge; Fladmark, Kari Espolin; Døskeland, Stein Ove. 2000. An ultrasensitive competitive binding assay for the detection of protein phosphatase directed toxins. Toxicon. 38: 347-360.
  • Fladmark, Kari Espolin; Brustugun, Odd Terje; Hovland, Randi; Bøe, Ragnar; Gjertsen, Bjørn Tore; Zhivotovsky, B; Døskeland, Stein Ove. 1999. Ultrarapid apoptosis inducted by phosphatase inhibitors. Cell Death Differ. Cell Death and Differentiation. 6: 1099-1108.
  • Jensen, PH; Fladmark, Kari Espolin; Gjertsen, Bjørn Tore; Vintermyr, Olav Karsten. 1999. Caspase I related protease inhibition retards the execution of okadaic acid and camptothecin induced apoptosis and PAI-2 selective cleavage, but not commitment to cell death in HL-60 cells. British Journal of Cancer. 79: 1685-1691.
  • Serres, Margrethe Hauge; Fladmark, Kari Espolin; Døskeland, Stein Ove. 1999. An ultrasensitive competitive binding assay for the detection of toxins affecting protein phosphatases. Toxicon. 38: 347-360.
  • Brustugun, Odd Terje; Fladmark, Kari Espolin; Døskeland, Stein Ove; Orrenius, S.; Zhivotovsky, B. 1998. Apoptosis induced by microinjection of cytochrome c is caspase-dependent and is inibited by Bcl-2. Cell Death and Differentiation. 5: 660-668.
  • Fladmark, Kari Espolin; Serres, Margrethe Hauge; Larsen, Nina Lied; Yasumoto, T.; Aune, T.; Døskeland, Stein Ove. 1998. Sensitive detection of apoptogenic toxins in suspension cultures of rat and salmon hepatocytes. Toxicon. 36: 1101-1114.
  • Fladmark, Kari Espolin; Gjertsen, Bjørn Tore; Døskeland, Stein Ove; Vintermyr, Olav Karsten. 1997. Fas/APO-1 (CD95)-Induced Apoptosis of Primary Hepatocytes Is Inhibited by cAMP. Biochemical and Biophysical Research Communications - BBRC. 232: 20-25.
  • Fladmark, Kari Espolin; Gjertsen, Bjørn Tore; Molven, Anders; Mellgren, Gunnar; Vintermyr, Olav Karsten; Døskeland, Stein Ove. 1997. Gap junctions and growth control in liver regeneration and in isolated rat hepatocytes. Hepatology. 25: 847-855.
  • Zom, M; Fladmark, Kari Espolin; Øgreid, D.; Jastorff, B; Døskeland, SO; Dostman, WRG. 1995. Ala%3%3%5 is essential for high-affinity cAMP-binding of both sites A and B of cAMP-dependent protein kinase type I. FEBS Letters. 362: 291-294.
  • Zorn, M; Fladmark, Kari Espolin; Øgreid, D.; Jastorff, B; Døskeland, SO; Dostmann, WRG. 1995. ALA-335 is essential for high-affinity cAMP-binding of both sites A and B of cAMP-dependent protein kinase type I. FEBS Letters. 362: 291-294.
Rapporter/avhandlinger
  • Rungruangsak-Torrissen, Krisna; Fismen, Lise; Garberg, Hilde Kristin; Satake, Masayuki; Fladmark, Kari Espolin. 2006. Shellfish toxin azaspiracid-1 induced changes in cytoskeletal proteins in neuronal cells. Institute of Marine Research, Matre Research Station,, Institute of Marine Research and University of Bergen. 28 sider.
  • Rungruangsak-Torrissen, Krisna; Garberg, Hilde Kristin; Thorsen, Anders; Kjesbu, Olav Sigurd; Fladmark, Kari Espolin. 2006. Oocyte development in Atlantic cod (Gadus morhua L.): Trypsin-like specific activity, capacity for protein synthesis and proteomics. Institute of Marine Research, Matre Research Station,, Institute of Marine Research and University of Bergen. 29 sider.
  • Fladmark, Kari Espolin. 1998. Hepatocyte proliferation and apoptosis-regulation by cell communication and phosphorylation. Dr.scient.. -.

Se fullstendig oversikt over publikasjoner i CRIStin.

Proteomic tools to elucidate the molecular action of microalgal toxins. In Toxins and biologically active compounds from microalgae. Ed Rossini GP Fladmark KE (2014) (Book chapter)

Identification of dynamic changes in proteins associated with the cellular cytoskeleton after exposure to okadaic acid. Opsahl JA, Ljostveit S, Solstad T, Risa K, Roepstorff P, and Fladmark KE (2013) Marine Drugs11:1763-82.

Comparative analysis of IgM sub-variants in salmonid fish and identification of a residue in m3 which is essential for MAb4C10 reactivity. KamilA, RaaeA, Fjelldal PG, Koppang EO, Fladmark KE, Hordvik I (2013)Fish and Shellfish Immunology 34: 667-72.

Nodularin exposure induces SOD1 phosphorylation and distrupts SOD1 co-localization with actin. Hjørnevik LV, Fismen L, Young FM, Solstad T , Fladmark KE (2012) . Toxins 4:1482-1499.

Dietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a comparative 2D-DIGE study. de Vareilles M, Conceição L, Gómez-Requeni P, Kousoulaki K, Richard N, Rodrigues P, Fladmark, KE, Rønnestad I, (2012) Marine Biotechnology 14:643-654.

Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae. de Vareilles M, Richard N, Gavaia PJ, Silva TS., Cordeiro O, Guerreiro I, Yúfera M, Batista I, Pires C, Pousão-Ferreira P, Rodrigues PM, Rønnestad I, Fladmark KE, Conceição LEC (2012) J Appl Ichthyol 28: 477-87.

Ectopic expression of Flt3 kinase inhibits proliferation and promotes cell death in different human cancer cell lines Oveland E, Wergeland L, Hovland R, Lorens JB, Gjertsen BT, Fladmark KE. Cell Biol Toxicol. 2012 Mar 16.

Increased interaction between DJ-1 and the Mi-2/ nucleosome remodelling and deacetylase complex during cellular stress. Opsahl JA, Hjornevik LV, Bull VH, Fismen L, Froyset AK, Gromyko D, Solstad T, Fladmark KE. Proteomics. 2010;10(7):1494-504.

Acyloxymethyl Esterification of Nodularin-R and Microcystin-LA Produces Inactive Protoxins that Become Reactivated and Produce Apoptosis inside Intact Cells. Herfindal L, Kasprzykowski F, Schwede F, Lankiewicz L, Fladmark KE, Lukomska J, Wahlsten M, Sivonen K, Grzonka Z, Jastorff B, Døskeland SO J Med Chem 2009 Epub 2009 aug.

Proteomic response of human neuroblastoma cells to azaspiracid-1. Kellmann R, Schaffner CA, Grønset TA, Satake M, Ziegler M, Fladmark KE. J Proteomics. 2009 May 2;72(4):695-707. Epub 2009 Mar 5.

Ligand-induced Flt3-downregulation modulates cell death associated proteins and enhances chemosensitivity to idarubicin in THP-1 acute myeloid leukemia cells. Oveland E, Gjertsen BT, Wergeland L, Selheim F, Fladmark KE, Hovland R. Leuk Res. 2009 Feb;33(2):276-87. Epub 2008 Aug 8.

Identification of a novel phosphorylation site of acyl-CoA binding protein (ACBP) in nodularin-induced apoptotic hepatocytes. Solstad T, Fismen L, Garberg H, Fladmark KE. Exp Cell Res. 2008 Jun 10;314(10):2141-9. Epub 2008 Mar 30.

Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Målen H, Berven FS, Fladmark KE, Wiker HG. Proteomics. 2007 May;7(10):1702-18.

CaM-kinaseII-dependent commitment to microcystin-induced apoptosis is coupled to cell budding, but not to shrinkage or chromatin hypercondensation. Krakstad C, Herfindal L, Gjertsen BT, Bøe R, Vintermyr OK, Fladmark KE, Døskeland SO. Cell Death Differ. 2006 Jul;13(7):1191-202. Epub 2005 Nov 25.

Algal toxins as guidance to identify phosphoproteins with key roles in apoptotic cell death. Solstad T, Fladmark KE. Curr Pharm Biotechnol. 2006 Jun;7(3):209-15. Review.

Proteomics approaches to elucidate oncogenic tyrosine kinase signaling in myeloid malignancies. Oveland E, Fladmark KE, Wergeland L, Gjertsen BT, Hovland R. Curr Pharm Biotechnol. 2006 Jun;7(3):185-98. Review.

Dr. scient, University of Bergen. “Hepatocyte proliferation and apoptosis: regulation by cell communication and phosphorylation” (1998)

Forskergrupper

Prosjekter

Pågående prosjekter:

  • "Role of phosphorylation in regulation of redox-mediated events in apoptotic cell death" (FUGE)
  • "Nordic Network in proteomics" (NordForsk) NORPRO
  • "Kompetanseoppbygging i kvantitativ proteomikk" (Meltzer)
  • "Nordic network in quantitative proteomics" (NordForsk) Q-NORDIC