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Nils Åge Frøysteins bilde

Nils Åge Frøystein

Førsteamanuensis
  • E-postNils.Froystein@uib.no
  • Telefon+47 55 58 35 46
  • Besøksadresse
    Realfagbygget, Allégt. 41
  • Postadresse
    Postboks 7803
    5020 BERGEN

Forskninga mi dreiar seg for ein stor del om bruk av høgoppløysande multikjerne (1H, 13C, 15N) og multidimensjonal (2D/3D) NMR-spektroskopi av isotopisk anrika (13C, 15N) protein i løysing og 2D NMR-spektroskopi av proteinbindande peptid. NMR-spektrene kan gje informasjon om struktur av molekyl, dynamiske prosessar (utveksling) og vekselverknader (binding) mellom til dømes store biomolekyl og mindre ligand molekyl. Eg er mest interessert i dei metodiske sidene av NMR-spektroskopi, og eg arbeider med NMR-spektroskopi innanfor mange bruksområde i kjemi.

Undervisning

Eg underviser følgjande kurs:

KJEM100 / Kjemi i naturen

KJEM306 / NMR-spektroskopi II

Masteroppgåver

NMR-spektrosopi av protein og peptid: Masteroppgåver blir tilbudt innanfor bruk av høgoppløysande multikjerne (1H, 13C, 15N og andre) og multidimensjonal (1D, 2D og 3D) NMR-spektroskopi brukt på protein, peptid og mindre molekyl som vekselverkar med protein. NMR-spektroskopi kan gje viktig informasjon om funksjon og virkemåte til/mekanismar for protein på molekylnivå i løysing. Dette kan vera strukturell informasjon for aktive sete, binding av peptid og småmolekylar (substrat, kofaktorar, inhibitorar og medisinmolekyl) og dynamiske prosessar (utveksling av små ligandmolekyl). Valg av NMR-metodar og etterprosesseringsprosedyrar vil vera sterkt avhengige av kva for type informasjon ein er ute etter i kvart tilfelle.

Denne forskinga er eit samarbeid med institutt for Molekylærbiologi.

Tidsskriftartikler
  • Brenner, Annette; Frøystein, Nils Åge. 2014. Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR. Molecules. 19: 8890-8903. doi: 10.3390/molecules19078890
  • Hahn, Friedrich; Setz, Christian; Friedrich, Melanie; Rauch, Pia; Solbak, Sara Marie Øie; Frøystein, Nils Åge; Henklein, Petra; Votteler, Jörg; Fossen, Torgils; Schubert, Ulrich. 2014. Mutation of the highly conserved ser-40 of the HIV-1 p6 gag protein to phe causes the formation of a hydrophobic patch, enhances membrane association, and polyubiquitination of gag. Viruses. 6: 3738-3765. doi: 10.3390/v6103738
  • Skår, Hjørdis; Seland, John Georg; Liang, Yucang; Frøystein, Nils Åge; Törnroos, Karl Wilhelm; Anwander, Reiner. 2013. Screening of the relaxivity of gadolinium-loaded periodic mesoporous silica functionalized by means of soft metalorganic silylamide grafting. European Journal of Inorganic Chemistry. 2013: 5969-5979. doi: 10.1002/ejic.201300756
  • Brenner, Annette; Kieffer, Bruno; Trave, Gilles Michel; Frøystein, Nils Åge; Raae, Arnt Johan. 2012. Thermal stability of chicken brain alpha-spectrin repeat 17: a spectroscopic study. Journal of Biomolecular NMR. 53: 71-83. doi: 10.1007/s10858-012-9620-y
  • Evjenth, Rune Henrik; Brenner, Annette; Thompson, Paul R.; Arnesen, Thomas; Frøystein, Nils Åge; Lillehaug, Johan R. 2012. Human Protein N-terminal Acetyltransferase hNaa50p (hNAT5/hSAN) Follows Ordered Sequential Catalytic Mechanism (combined kinetic and NMR study). Journal of Biological Chemistry. 287: 10081-10088. doi: 10.1074/jbc.M111.326587
  • Alvheim, Cecilie; Frøystein, Nils Åge; Vinje, Jo; Intini, Francesco P.; Natile, Giovanni; Liu, Yangzhong; Huang, Rongong; Sletten, Einar. 2009. Cytotoxic trans-oriented iminoether platinum complexes - Kinetics of binding to DNA oligonucleotides determined by 15-N NMR spectroscopy. Inorganica Chimica Acta. 362: 907-914. doi: 10.1016/j.ica.2008.01.041
  • Occhipinti, Giovanni; Jensen, Vidar Remi; Törnroos, Karl Wilhelm; Frøystein, Nils Åge; Bjørsvik, Hans-Rene. 2009. Synthesis of a new bidentate NHC–Ag(I) complex and its unanticipated reaction with the Hoveyda–Grubbs first generation catalyst. Tetrahedron. 65: 7186-7194. doi: 10.1016/j.tet.2009.05.095
  • Sommerfeldt, Hanne-Marthe; Meerman, Christian; Schrems, Marcus; Törnroos, Karl Wilhelm; Frøystein, Nils Åge; Miller, Robert J.; Scheidt, Ernst-Wilhelm; Scherer, Wolfgang; Anwander, Reiner. 2008. Characterization and reactivity of peralkylated Ln(II)Al(III) heterobimetallic complexes. Dalton Transactions. 2008: 1899-1907. doi: 10.1039/b716603f
  • Zimmermann, Melanie; Frøystein, Nils Åge; Fischbach, Andreas; Sirsch, Peter; Dietrich, Hans Martin; Törnroos, Karl Wilhelm; Herdtweck, Eberhardt; Anwander, Reiner. 2007. Homoleptic rare-earth metal(III) tetramethylaluminates: Structural chemistry, reactivity, and performance in isoprene polymerization. Chemistry - A European Journal. 13: 8784-8800. doi: 10.1002/chem.200700534
  • Grove, Hilde; Frøystein, Nils Åge; Sæthre, Leif J; Sletten, Jorunn. 2006. Crystalline products isolated from solutions with commercially available 2,3-bis(2-pyridyl)pyrazine (dpp) as reactant: Detection of a dimerized form of dpp. Journal of Molecular Structure. 800: 1-17.
  • Halskau, Øyvind; Underhaug, Jarl; Frøystein, Nils Åge; Martinez, Aurora. 2005. Conformational flexibility of alpha-lactalbumin related to its membrane binding capacity. Journal of Molecular Biology. 349: 1072-1086. doi: 10.1016/j.jmb.2005.04.020
  • Teigen, Knut; Dao, Khanh Kim; Mckinney, Jeffrey Alan; Gorren, A.C.F.; Mayer, B.; Frøystein, Nils Åge; Haavik, Jan; Martinez, Aurora. 2004. Tetrahydrobiopterin binding to aromatic amino acid hydroxylases. Ligand recognition and specificity. Journal of Medicinal Chemistry. 47: 5962-5971.
  • Doudin, K.; Berge, Rolf Kristian; Frøystein, Nils Åge; Songstad, Jon. 2000. Preparation and NMR studies of 3-selenaheptadecanoic and 4-selenaoctadecanoic acid, RSe(CH2)nC(O)OH (R = -C14H29, n = 1 and 2), and their methyl esters. Journal of the Chemical Society. Perkin Transactions 2 (2001). 1: 723-729.
  • Teigen, Knut; Frøystein, Nils Åge; Martinez, Aurora. 1999. The structural Basis of the Recognition of Phenylalanine and Pterine Cofactors by Phenylalanine Hydroxylase. Implications for the Catalytic Mechanism. Journal of Molecular Biology. 294: 807-823.
  • Teigen, Knut; Frøystein, Nils Åge; Martinez, Aurora. 1999. The structural basis of the recognition of phenylalanine and pterin cofactors by phenylalanine hydroxylase. Implications for the catalytic mechanism. Journal of Molecular Biology. 294: 807-823.
  • Fossen, Torgils; Frøystein, Nils Åge; Andersen, Øyvind Moksheim. 1998. Myricetin 3-rhamnopyranosyl (1-6) galactoside from Nymphaea x marliacea. Phytochemistry. 49: 1997-2000.
  • Moldrheim, Erlend; Andersen, Bjørn; Frøystein, Nils Åge; Sletten, Einar. 1998. Interaction of Manganese (II)Cobalt(II) and Nickel(I) with DNA Oligomers Studied by Proton NMR Spectroscopy. Inorganica Chimica Acta. 273: 41-46.
  • Garoufis, A.; Perlepes, S.P.; Frøystein, Nils Åge; Sletten, Jorunn; Hadjiliadis, J. 1996. Mercuration of acetone in the acetone-mercury(II) perchlorate2-('2-pyridyl)quinoxaline(L) system. Polyhedron. 15: 1035-1039.
  • Frøystein, Nils Åge; Sletten, Einar. 1994. The binding of mercury(II) to the syntetic oligonucleotide d(CGcGAATTCGCG)%cA %1H adn %1%5N NMR study. Journal of the American Chemical Society. 116: 3240-3250.
  • Frøystein, Nils Åge; Davis, J. T.; Reid, B. R.; Sletten, Einar. 1993. Sequence-Selective Metal Ion Binding to DNA Oligonucleotides. Acta Chemica Scandinavica. 47: 649-657.
  • Steinkopf, Signe; Frøystein, Nils Åge; Liu, Q.; Sletten, Einar. 1992. Copper(II) - Purine Complexes Studied by Proton and Nitrogen-15 Nuclear Magnetic Resonance. Acta Chemica Scandinavica. 46: 446-450.
  • Stavropoulos, George; Karagiannis, Kostas; Vynios, Dimitrios; Papaioannou, Dionissios; Aksnes, Dagfinn; Frøystein, Nils Åge; Francis, George W. 1991. Solid-Phase Synthesis and Spectroscopic Studies of TRH Analogues Incorporating Cis- and trans-4-Hydroxy-L-Proline. Acta Chemica Scandinavica. 45: 1047-1054.
Rapporter/avhandlinger
  • Frøystein, Nils Åge. 1993. Sequence-selective interactions of DNA and metal ions : NMR studies of the interaction of divalent transition metal ions with oligodeoxyribonucleotides. Department of Chemistry, University of Bergen, Bergen. 1 sider.
Bokkapitler
  • Sletten, Einar; Frøystein, Nils Åge. 2009. Sequence-Selective Binding of Transition Metal Complexes to DNA. Chap. 1, sider 3-30. I:
    • Hadjiliadis, Nick; Sletten, Einar. 2009. Metal Complex - DNA Interactions. John Wiley & Sons. 524 sider. ISBN: 978-1-4051-7629-3.
  • Sletten, Einar; Frøystein, Nils Åge. 1996. NMR studies of oligonucleotide - metal ion complexes. Chap. 11, 397. I:
    • Sigel, H.; Sigel, S. 1996. Metal ions in biological systems.
  • Sletten, Einar; Frøystein, Nils Åge. 1996. NMR Studies of Oligonucleotide-Metal Ion Interactions. 397-415. I:
    • Sigel, Helmut; Sigel, Astrid. 1996. Metal Ions in Biological Systems. ISBN: 0-8247-9549-0.
  • Sletten, Einar; Frøystein, Nils Åge. 1995. NMR spectroscopic structure determination of metal ion - oligonucleotide complexes - the results of sequence-selective binding studies. 144-151. I:
    • Kessissoglou, Dimitris P. 1995. Bioinorganic chemistry : an inorganic perspective of life ( NATO ASI series. Series C, Mathematical and physical scienc es ; vol. 459). ISBN: 0-7923-3380-2.

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