Øyvind Halskaus bilde
Øyvind
Halskau
Førsteamanuensis
Besøksadresse: 
HIB - Thormøhlensgt. 55
5020 Bergen
Postadresse: 
Postboks 7803
5020 Bergen
Telefon: 
+47 55 58 45 63
Last ned visittkort

Proteinkjemi - evolusjon, struktur, folding og dynamikk relatert til funksjon.

HUB310; Vitenskapsteori for masterstudenter.

Tidsskriftartikler
  • Skjevik, Åge Aleksander; Mileni, Mauro; Baumann, Anne; Halskau, Øyvind; Teigen, Knut; Raymond C., Stevens; Martinez, Aurora. 2014. The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes. Journal of Molecular Biology. 426: 150-168. doi: 10.1016/j.jmb.2013.09.012
  • Havukainen, Heli; Münch, Daniel; Baumann, Anne; Zhong, Shi; Halskau, Øyvind; Krogsgaard, Michelle; Amdam, Gro Vang. 2013. Vitellogenin recognizes cell damage through membrane binding and shields living cells from reactive oxygen species. Journal of Biological Chemistry. 288: 28369-28381. doi: 10.1074/jbc.M113.465021
  • Lystvet, Sina Maria; Volden, Sondre; Singh, Gurvinder; Rundgren, Ida Marie; Wen, Hanzhen; Halskau, Øyvind; Glomm, Wilhelm. 2013. Anticancer Activity from Gold-alpha-Lactalbumin Nanoconstructs? The Journal of Physical Chemistry C. 117: 2230-2238. doi: 10.1021/jp3104886
  • Lystvet, Sina Maria; Volden, Sondre; Singh, Gurvinder; Yasuda, Masahiro; Halskau jr, Øyvind; Glomm, Wilhelm. 2013. Tunable photophysical properties, conformation and function of nanosized protein–gold constructs. RSC Advances. 3: 482-495. doi: 10.1039/c2ra22479h
  • Solbak, Sara Marie Øie; Reksten, Tove Ragna; Hahn, Friedrich; Wray, Victor; Henklein, Petra; Henklein, Peter; Halskau, Øyvind; Schubert, Ulrich; Fossen, Torgils. 2013. HIV-1 p6 - a structured to flexible multifunctional membrane-interacting protein. Biochimica et Biophysica Acta - Biomembranes. 1828: 816-823. doi: 10.1016/j.bbamem.2012.11.010
  • Wen, Hanzhen; Glomm, Wilhelm; Halskau, Øyvind. 2013. Cytotoxicity of bovine alpha-lactalbumin: Oleic acid complexes correlates with the disruption of lipid membranes. Biochimica et Biophysica Acta - Biomembranes. 1828: 2691-2699. doi: 10.1016/j.bbamem.2013.07.026
  • Baumann, Anne; Gjerde, Anja Underhaug; Ying, Ming; Svanborg, Catharina; Holmsen, Holm A; Glomm, Wilhelm; Martinez, Aurora; Halskau jr, Øyvind. 2012. HAMLET forms annular oligomers when deposited with phoshpolipid monolayers. Journal of Molecular Biology. 418: 90-102. doi: 10.1016/j.jmb.2012.02.006
  • Bustad, Helene J.; Skjærven, Lars; Ying, Ming; Halskau jr, Øyvind; Baumann, Anne; Rodriguez-Larrea, David; Costas, Miguel; Underhaug, Jarl; Sanchez-Ruiz, Jose Manuel; Martinez, Aurora. 2012. The peripheral binding of 14-3-3gamma to membranes involves isoform-specific histidine residues. PLoS ONE. 7. doi: 10.1371/journal.pone.0049671
  • Havukainen, Heli; Underhaug, Jarl; Wolschin, Florian; Amdam, Gro Vang; Halskau jr, Øyvind. 2012. A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation. Journal of Experimental Biology. 215: 1837-1846. doi: 10.1242/jeb.065623
  • Schillinger, Anne-Sophie; Grauffel, Cédric; Halskau, Øyvind; Reuter, Nathalie. 2012. PR3 Interacts Directly to Lipid Bilayers: Evidence from MD Simulations and SPR Experiments. Biophysical Journal. 102: 497A-497A.
  • Volden, Sondre; Lystvet, Sina Maria; Halskau jr, Øyvind; Glomm, Wilhelm. 2012. Generally applicable procedure for in situ formation of fluorescent protein-gold nanoconstructs. RSC Advances. 2: 11704-11711. doi: 10.1039/c2ra21931j
  • Glomm, Wilhelm; Volden, Sondre; Ese, Marit-Helen; Halskau jr, Øyvind. 2011. Interactions of α-Lactalbumin and Cytochrome с with Langmuir Monolayers of Glycerophospholipids. Journal of Dispersion Science and Technology. 32: 150-158. doi: 10.1080/01932690903543287
  • Havukainen, Heli; Halskau jr, Øyvind; Amdam, Gro Vang. 2011. Social pleiotropy and evolution of honey bee vitellogenin. Molecular Ecology. 20: 5111-5113. doi: 10.1111/j.1365-294X.2011.05351.x
  • Havukainen, Heli; Halskau jr, Øyvind; Skjærven, Lars; Smedal, Bente; Amdam, Gro Vang. 2011. Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminus. Journal of Experimental Biology. 214: 582-592. doi: 10.1242/jeb.048314
  • Johannessen, Helene Bustad; Underhaug, Jarl; Halskau jr, Øyvind; Martinez, Aurora. 2011. The binding of 14-3-3 gamma to membranes studied by intrinsic fluorescence spectroscopy. FEBS Letters. 585: 1163-1168. doi: 10.1016/j.febslet.2011.03.027
  • Lystvet, Sina Maria; Volden, Sondre; Halskau jr, Øyvind; Glomm, Wilhelm. 2011. Immobilizaton onto gold nanoparticles alters α-lactalbumin interaction with pure and mixed phospholipid monolayers. Soft Matter. 7: 11501-11509. doi: 10.1039/c1sm06337e
  • Lystvet, Sina Maria; Volden, Sondre; Yasuda, Masahiro; Halskau jr, Øyvind; Glomm, Wilhelm. 2011. Emergent membrane-affecting properties of BSA-gold nanoparticle constructs. Nanoscale. 3: 1788-1797. doi: 10.1039/c0nr00948b
  • Wen, Hanzhen; Rundgren, Ida M.; Glomm, Wilhelm; Halskau jr, Øyvind. 2011. Benchmarking Different BAMLET-like Preparations with Resepct to Tryptophan Exposure, Interfacial Activity, and Effect on Cell Viability. Bioanalysis & Biomedicine. 5 sider. doi: 10.4172/1948-593X.S5-003
  • Halskau jr, Øyvind; Volden, Sondre; Calvo, Ana C; Martinez, Aurora; Glomm, Wilhelm. 2010. Adsorption and bioactivity of tyrosine hydroxylase on gold surfaces and nanoparticles. Protein peptide letters. 17: 1376-1382.
  • Mossberg, Ann-Kristin; Puchades, Maja; Halskau jr, Øyvind; Baumann, Anne; Lanekoff, Ingela; Chao, Yinxia; Martinez, Aurora; Svanborg, Catharina; Karlsson, Roger. 2010. HAMLET interacts with lipid membranes and perturbs their structure and integrity. PLoS ONE. 5. 10 sider. doi: 10.1371/journal.pone.0009384
  • Glomm, Wilhelm Robert; Volden, Sondre; Halskau, O; Halskau jr, Øyvind; Ese, Marit-Helen G. 2009. Same System-Different Results: The Importance of Protein. Introduction Protocols in Langmuir-Monolayer Studies of Lipid-Protein Interactions. Analytical Chemistry. 81: 3042-3050. doi: 10.1021/ac8027257
  • Halskau jr, Øyvind; Muga, Arturo; Martinez, Aurora. 2009. Linking new paradigms in protein chemistry to reversible membrane-protein interactions. Current protein and peptide science. 10: 339-359.
  • Halskau jr, Øyvind; Ying, Ming; Baumann, Anne; Kleppe, Rune; Rodriguez-Larrea, David; Almås, Bjørg; Haavik, Jan; Martinez, Aurora. 2009. Three-way interaction between 14-3-3 proteins, the N-terminal region of tyrosine hydroxylase and negatively charged membranes. Journal of Biological Chemistry. 284: 32758-32769. doi: 10.1074/jbc.M109.027706
  • Sælensminde, Gisle; Halskau jr, Øyvind; Jonassen, Inge. 2009. Amino acid contacts in proteins adapted to different temperatures: hydrophobic interactions and surface charges play a key role. Extremophiles. 13: 11-20. doi: 10.1007/s00792-008-0192-4
  • Halskau jr, Øyvind; Perez-Jimenez, Raul; Ibarra-Molero, Beatriz; Underhaug, Jarl; Muñoz, Victor; Martinez, Aurora; Sanchez-Ruiz, Jose Manuel. 2008. Large-scale modulation of thermodynamic protein folding barriers linked to electrostatics. Proceedings of the National Academy of Science of the United States of America. 105: 8625-8630. doi: 10.1073/pnas.0709881105
  • Sælensminde, Gisle; Halskau jr, Øyvind; Jonassen, Inge. 2008. Amino acid contacts in proteins adapted to different temperatures: hydrophobic interactions and surface charges play a key role. Extremophiles. doi: 10.1007/s00792-008-0192-4
  • Glomm, Wilhelm R; Halskau jr, Øyvind; Halskau, Jr., Øyvind; Hanneseth, Ann-Mari D; Volden, Sondre. 2007. Adsorption Behavior of Acidic and Basic Proteins onto Citrate-Coated Au Surfaces Correlated to Their Native Fold, Stability, and pI. Journal of Physical Chemistry B. doi: 10.1021/jp074839d
  • Sælensminde, Gisle; Saelensminde, G; Halskau, Øyvind; Halskau, O; Helland, Ronny; Willassen, Nils Peder; Jonassen, I. 2007. Structure-dependent relationships between growth temperature of prokaryotes and the amino acid frequency in their proteins. Extremophiles. 11: 585-596. doi: 10.1007/s00792-007-0072-3
  • Halskau, Øyvind; Underhaug, Jarl; Frøystein, Nils Åge; Martinez, Aurora. 2005. Conformational flexibility of alpha-lactalbumin related to its membrane binding capacity. Journal of Molecular Biology. 349: 1072-1086. doi: 10.1016/j.jmb.2005.04.020
  • Rødland, Ingunn; Halskau, Øyvind; Martinez, Aurora; Holmsen, Holm A. 2005. alpha-Lactalbumin binding and membrane integrity--effect of charge and degree of unsaturation of glycerophospholipids. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 1717: 11-20.
  • Rødland, Ingunn; Halskau, Øyvind; Martinez, Aurora; Holmsen, Holm A. 2005. α-Lactalbumin binding and membrane integrity – effect of charge and degree of unsaturation of glycerophospholipids. Biochimica et Biophysica Acta - Biomembranes. 1717: 11-20. doi: 10.1016/j.bbamem.2005.09.004
  • Agasøster, Armelle, V.; Halskau, Øyvind; Fuglebakk, Edvin; Frøystein, Nils Å.; Muga, Arturo; Holmsen, Holm A; Martinez, Aurora. 2003. The interaction of peripheral proteins and membranes studied with alpha-lactalbumin and phospholipid bilayers of various compositions. Journal of Biological Chemistry. 278: 21790-21797.
  • Halskau, Øyvind; Frøystein, Nils Å.; Martinez, Aurora. 2002. Detaljer i utilgjengelig systemer – membran-protein vekselvirkninger. NBS-nytt. 26: 16-19.
  • Halskau, Øyvind; Frøystein, Nils Å.; Muga, Arturo; Martinez, Aurora. 2002. The Membrane-bound Conformation of a-Lactalbumin Studied by NMR-monitored 1H Exchange. Journal of Molecular Biology. 321: 99-110.
Rapporter/avhandlinger
  • Halskau jr, Øyvind. 2005. Structure and dynamics of the peripheral protein Lactalbumin in relation to its membrane binding capability.
  • Halskau, Øyvind. 2000. The membrane bound conformation of bovine alpha-lactalbumin studied by NMR-monitored 1H-exchange. Kjemisk institutt, UiB, Kjemisk institutt, UiB. 152 sider.

Se fullstendig oversikt over publikasjoner i CRIStin.

<!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0mm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 70.85pt 70.85pt 70.85pt; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> 2. Sælensminde, G., Halskau, Ø., and Jonassen, I. (2009) Amino acid contacts in proteins adapted to different temperatures: hydrophobic interactions and surface charges play a key role. Extremophiles 13, 11-20

3. Halskau, Ø., Muga, A., and Martínez, A. (2009) Linking new paradigms in protein chemistry to reversible membrane-protein interactions Curr. Prot. Pept. Sci. Accepted

4. Halskau, Ø., Underhaug, J., Sanchez-Ruiz, J. M., Ibarra-Molero, B., Muñoz, V., and Martínez, A. (2008) Large-scale modulation of thermodynamic protein folding barriers linked to electrostatics. Proc. Natl. Acad. Science 105, 8625-8630

5. Sælensminde, G., Halskau, Ø., Helland, R., Willassen, N. P., and Jonassen, I. (2007) Structure-dependent relationships between growth temperature of prokaryotes and the amino acid frequency in their proteins. Extremophiles 11, 585-596

6. Glomm, W. R., Halskau, Ø., Hanneseth, A. M., and Volden, S. (2007) Adsorption behavior of acidic and basic proteins onto citrate-coated Au surfaces correlated to their native fold, stability, and pI. J Phys Chem B 111, 14329-14345

7. Rødland, I.*, Halskau, Ø.*, Martínez, A., and Holmsen, H. (2005) alpha-Lactalbumin binding and membrane integrity--effect of charge and degree of unsaturation of glycerophospholipids. Biochim Biophys Acta 1717, 11-20

8. Halskau, Ø., Underhaug, J., Frøystein, N. A., and Martínez, A. (2005) Conformational flexibility of alpha-lactalbumin related to its membrane binding capacity. J Mol Biol 349, 1072-1086

9. Agasøster, A. V.*, Halskau, Ø.*, Fuglebakk, E., Frøystein, N. A., Muga, A., Holmsen, H., and Martínez, A. (2003) The interaction of peripheral proteins and membranes studied with alpha-lactalbumin and phospholipid bilayers of various compositions. J Biol Chem 278, 21790-21797

10. Halskau, Ø., Frøystein, N. A., Muga, A., and Martínez, A. (2002) The membrane-bound conformation of alpha-lactalbumin studied by NMR-monitored 1H exchange. J Mol Biol 321, 99-110

- Halskau, Ø., Ying, M., Baumann, A., Kleppe, R., Larrea, D. R., Almås, B., Haavik, J., and Martínez, A. (2008) Three-way interaction between 14-3-3 proteins, the N-terminal region of tyrosine hydroxylase and negatively charged membranes. J Biol Chem Revised submission

- Mossberg, A., Baumann, A., Halskau, Ø., Svanborg, C., and Martínez, A. (2009) Membrane affinity of HAMLET mediates its apoptotic specificity. Manuscript

* First authorship shared

 

 

 

NMR og annen spektroskopi (Fluorescence, CD m.m.). Membran- og overflate relaterte teknikker slik som liposomer, Langmuir, Lanmuir-Blodget, samt Atomic Force Microscopi og Quarts Crystal Microbalance (i samarbeid med NTNU). Computerassistert analyse av eksperimentelle data, for eksempel mulit-dimensjonelle NMR-data og Differential Scanning Calorimetri-data.

Prosjekter

2009: Interfaces as folding templates for polypetides (With associate professor Wilhelm Glomm)

2008: From details to drugs – a thorough structural and dynamic analysis of 14-3-3, tyrosine hydroxylase and membranes (Lie and Jensens fund/Norwegian Cancer Society, grant #58240001, with Professor Aurora Martínez)

2007: Dissecting molecular properties of honey bee vitellogenin (Research Council of Norway, grant #185306, with Professor Gro Vang Amdam)

2006: Linking new paradigms in protein chemistry to membrane-protein interaction, apoptosis and signalling (Norwegian Cancer Society, grant #06109/01, with Professor Aurora Martínez)

2002: Structural characterization of protein folding variants that induce apoptosis in tumor cells (Research Council of Norway, grant #149117, with Professor Aurora Martínez)