Anne Paulus Døskeland
Senior Engineer
The Department of Biomedicine
Title: Senior Engineer
Phone: +47 55 58 61 48
E-mail: Anne.Doskeland@biomed.uib.no
Visiting address: Jonas Lies vei 91
Dr. Anne P. Døskeland (Cand real, PhD) is employed at the Proteomics Unit at the University of Bergen Master Degrees in Chemistry and Medical Sciences (1973) were obtained at the Sciences and Medical Faculties of the University of Louvain, Belgia. PhD (1991) was obtained at the Institute of Biochemistry, University of Bergen, Norway.
Anne is affiliated to the facility service PROBE and responsible for the mass spectrometric analysis of samples delivered by clients. Most of studies consist in identification of proteins by MALDI-TOF-TOF or LCMSMS.
Her main research area is related to the regulation of the ubiquitin-proteasome pathway.
Induction of apoptosis by proteasome inhibitors (bortezomib, PR171) has been characterized in leukemia cells, and a proteome/degradome profiling of proteins affected by drugs bortezomib and anthracyclin is studied using mass spectrometric methods. Prof. J. Vandekerckhove has contributed to the work with its expertise in Cofradic methodology.
Studied subjects related to the Ubiquitin-proteasome pathway
- Conjugation of ubiquitin to substrates phenylalanine and tyrosine hydroxylases, and their degradation by the proteasome
- Mass spectrometric analysis by MALDI of polyubiquitin chains
- The Ub-proteasome pathway regulation: its inhibition by bortezomib and PR171 in leukemia cells, and MS/MS analysis of the degradome in order to define the proteins affected by the drugs in the apoptotic process.