BBB Seminar: Nicolai Miosge - Cancelled
Migratory progenitor cells in osteoarthritis and rheumatoid arthritis are driven by inflammatory mediators and sex hormones
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NB! Cancelled due to uncertainties with flight schedules after the Icelandic volcano eruption
Nicolai Miosge
Tissue Regeneration Group, Department of Prosthodontics, Georg-August-University Göttingen, Germany
The regeneration of diseased hyaline cartilage continues to be a great challenge, mainly because degeneration – caused either by major injury or by age-related processes – can overextend the tissue’s self-renewal capacity.
Samples of osteoarthritic cartilage and rheumatoid arthritic cartilage were obtained from the knee joints of patients (ages: 65-75 years) suffering from late-stage osteoarthritis (OA) or rheumatoid arthritis (RA) after total knee replacement. Light microscopy, ultrastructural investigation, cell isolation, cloning and immortalization, as well as multipotent differentiation experiments were performed. Furthermore, quantitative real-time RT-PCR, Western blotting, SILAC proteomics as well as RNA interference were applied.
We have recently shown that repair tissue from late stages of osteoarthritis in humans harbors a unique progenitor cell population, termed chondrogenic progenitor cells (CPCs). These exhibit stem cell characteristics such as clonogenicity, multipotency, and migratory activity. CPCs are governed by the osteogenic transcription factor runx-2 and the chondrogenic transcription factor sox-9. They show gender differences and exhibit estrogen and progesterone receptors. Treatment, especially with estrogen, at least in vitro, can enhance their chondrogenic potential. We have now isolated a similar CPC population from RA cartilage tissue.
Our results offer new insights into the biology of progenitor cells in the context of diseased cartilage tissue and are relevant in the development of novel therapeutics for osteoarthritis and rheumatoid arthritis.
Selected publications:
Koelling S, Kruegel J, Irmer M, Path JR, Sadowski B, Miro X, Miosge N (2009) Migratory chondrogenic progenitor cells from repair tissue during the late stages of human osteoarthritis. Cell Stem Cell. 4:324-35.
Koelling S, Miosge N (2010) Sex differences of chondrogenic progenitor cells in late stages of osteoarthritis. Arthritis Rheum. 64:1077-87.
Koelling S, Miosge N (2009) Stem cell therapy for cartilage regeneration in osteoarthritis. Expert Opin Biol Ther. 9:1399-405.
Host: Donald Gullberg, Department of Biomedicine