BBB Seminar: Boris Hinz
Myofibroblasts and the matrix
Laboratory of Tissue Repair and Regeneration, Matrix Dynamics Group, University of Toronto, ON, Canada
The high contractile force generated by myofibroblasts is beneficial for physiological tissue repair but detrimental for organ function when it becomes excessive such as in hypertrophic scars, in virtually all fibrotic diseases and during the stroma reaction to tumors. Myofibroblasts not only exert force but adapt to the stress in their microenvironment. We modulate myofibroblast tension using multiple techniques, including collagen gels of different stiffness, novel substrates with tunable compliance, stretchable culture membranes and cell shape restriction by microcontact-printing. On the molecular level, we specifically interfere with the contractile apparatus, matrix-adhesion, cell-adhesion and growth factor activation of myofibroblasts. In general, stress-release leads to de-differentiation and/or apoptosis of myofibroblasts. We propose therapeutically interfering with their stress-perception and -transmission apparatus as novel strategy to eliminate fibrogenic cells.
Host: Donald Gullberg, Department of Biomedicine