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The Department of Biomedicine

BBB Seminar: Ben N. G. Giepmans

Fluorescence microscopy meets electron microscopy

The 5th NorMIC Symposium (invited speaker)

Ben N. G. Giepmans
Department of Cell Biology, University Medical Center Groningen, University of Groningen, The Netherlands

Understanding where, when, and how biomolecules (inter)act is crucial to uncover fundamental mechanisms in cell biology. Fluorescence microscopy allows protein imaging in living cells and at the near molecular level. However, fluorescence microscopy only reveals selected biomolecules or organelles but not the (ultra)structural context, as can be examined by electron microscopy (EM). Correlated light and electron microscopy (CLEM) of the same specimens allows the examination of rare or dynamic events first by light microscopy, and subsequently by EM. Today, I will discuss (i) New versus old probes in CLEM; (ii) Implementation of CLEM in diverse cell biological projects; (iii) Large scale EM (“nanotomy”) during Type 1 diabetes onset that provides structural insight into destruction of Islets of Langerhans, beta cells and organelles. The current developments will revolutionize and standardize CLEM, which is likely to become a routine technique in biology.

Host: Jaakko Saraste , Department of Biomedicine