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The Department of Biomedicine

BBB Seminar: Fatima H. Labeed - Cancelled!

Dielectrophoresis: a non-invasive electrophysiological tool for cellular analysis

Fatima H. Labeed, Centre for Biomedical Engineering, School of Engineering, University of Surrey, Guildford, UK

Dielectrophoresis (DEP) is an electrostatic phenomenon, defined as the motion of particles resulting from polarisation forces produced by an inhomogeneous electric field. DEP has been used for 40 years for the manipulation, separation and analysis of cells.

At the University of Surrey, we are specialists in extracting the electrophysiological parameters (or “fingerprints”) of cells non-invasively. Some of those parameters include; cytoplasmic conductivity (relates to cytoplasm ionic strength), membrane conductance (describes the ionic transport across the membrane), and specific membrane capacitance (relates to membrane morphology). The technique and technology have been used in many different cellular settings, examples include multidrug resistance in leukaemic and breast cancer cell lines before and after treatment with anticancer agents, rapid early apoptosis detection by means of detecting associated electrophysiological characteristics over time and onset of late apoptosis, studying ion channel activity by means of using ion channel blockers, using DEP as a biomarker for neurostem cells differentiation potentials and studying oral cancer and the technique potential for early detection.

This talk will describe how the technique works, and our current development of the technology to provide rapid electrophysiological fingerprinting of cells, discussing the cellular applications mentioned above in more detail. The results of these studies will be discussed and will demonstrate the potential use of this technique as a detection and separation tool.

Chair: Anne C. Johannessen, The Gade Institute

 

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