Objectives and Content
The course consist of two parts:
Spectroscopy, 2 sp
Biochemical methodology, 1 sp
This part entails spectroscopic techniques used in the analysis of organic compounds. Infrared (IR) and ultraviolet (UV) spectroscopy will be discussed, while the main focus will be on nuclear magnetic resonance (NMR) and mass spectrometry (MS). Students will learn how to identify unknown compounds by using information from IR, UV, MS and NMR spectra.
Relevant pharmaceutical examples will be discussed.
The course includes the theory and practical aspects of some basic techniques in molecular biology and biochemistry. The main focus is on purification of plasmids, PCR, restriction enzyme digestion, transformation of bacteria, recombinant protein expression, purification of His-tagged proteins, SDS-PAGE and a modification of Western blotting.
Explain/discuss the different spectroscopic techniques (IR, UV/vis, NMR and MS) and interpret spectroscopic data from IR, UV/vis, NMR and MS either by themselves of combinations of these in order to determine the structures of organic compounds.
Master the basic knowledge behind the practical methods that are used in this course, be able to perform basic cloning techniques, PCR and to express recombinant protein in bacteria and to perform the SDS gel electrophoresis and Western / spot blot techniques. The student should be able to master basic molecular biological techniques in general and be able to carry out similar experiments
Recommended Previous Knowledge
Biochemical methodology: FARM150
Compulsory Assignments and Attendance
It is mandatory to be present and participate in the biochemical methodology part of the course.
More information is given under assessment.
Forms of Assessment
Spectroscopy: Written exam (2 hours). Grading scale A-F
Biochemical methodology: Mandatory participation in course. Grading: Passed/fail.
The total grade for FARM301A will be the grade given for the Spectroscopy exam, as biochemical methodology only gives the grade passed/fail.
Permitted examination support materials, Spectroscopy: The textbook (Williams and Fleming: Spectroscopic methods in organic chemistry 6th Edition.). It is not allowed to have notes in the textbook, which can be used on the exam. Underlining of text, figures and tables in the textbook are allowed.
Spectroscopy: grading scale A-F is used.
Biochemical methodology: mandatory participation in course. Grading: Passed/fail
Department of Clinical Science
For written exams, please note that the start time may change from 09:00 to 15:00 or vice versa until 14 days prior to the exam.
Type of assessment: Written, assignment, written
- Withdrawal deadline
Exam part: Spektroskopi
- 03.05.2021, 09:00
- 2 hours
- Examination system
- Digital exam
Exam part: Biokjemisk metodikk