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Postgraduate course

Molecular and Cellular Methods in Immunology

  • ECTS credits10
  • Teaching semesterSpring, Autumn
  • Course codeHUIMM306
  • Number of semesters1
  • LanguageEnglish.
  • Resources

ECTS Credits

10

Level of Study

Master.

Teaching semester

Spring 2020: course will only be arranged if at least 2 students sign up for the course.

Maximum capacity is 6 students for both HUIMM306 & HUIMM906.

Course-coordinator Karl A. Brokstad decides who can participate.

Autumn 2021: Course will only be arranged for min. 4 students and max 8 students, august - september.

Maximum capacity is 8 students for both HUIMM306 & HUIMM906.

Place of Instruction

Broegelmann Research Laboratory, Department of Clinical Science.

Lab.building/ BB-building & Haukeland University Hospital.

Objectives and Content

Aim:

Introduction to some general methods used in immunological research by using them actively in

the lab. The practical part of the course should enable the students to understand methods used in

scientific papers. In addition, the methods used will be introduced in seminars by the course leaders.

Contents:

Theoretical part:

-The course leaders will give a theoretical introduction into some of the most commonly used immunological methods.

Practical part:

-Calculation of molarities, preparation of buffers

- Isolation of leukocytes from blood using density gradient centrifugation.

Isolation and cultivation of monocytes (cell culture), in vitro generation of macrophages.

- Western blot analysis of signaling pathways using stimulated cells.

- ELISA. Quantificatin of cytokins produced by the cells.

- Immunostaining. Visualisation using fluorescently labeled secondary antibody.

- Quantification of copy number using PCR: Comparison of standard PCR with quantitative real time PCR

Learning Outcomes

On completion of the course the student is able to/has:

Knowledge

-advanced knowledge of the most commonly used immunological methods and understand the aim and theory behind those techniques.

Skills

- explain the appropriate use of different immunological methods and can independently use those used in the course

- critically evaluate own results

Competence

- apply the acquired knowledge and skills to engage in further tasks related to research

- communicate results in a scientific way, both orally and in writing

Required Previous Knowledge

Some background in cellular and molecular biology. Experience with basic laboratory techniques such as pipetting and centrifugation. Priority will be given to students having taken the related course Human Immunology (HUIMM903/HUIMM303).

Recommended Previous Knowledge

Biology, biomedicine or molecular biology on bachelor level.

Credit Reduction due to Course Overlap

BMED320 (-5 ECTS).

Access to the Course

Spring 2020: course will only be arranged if at least 2 students sign up for the course.

Maximum capacity is 6 students for both HUIMM306 & HUIMM906.

Course-coordinator Karl A. Brokstad decides who can participate.

Teaching and learning methods

Theoretical introduction to the lab (1.5 h)

10 days in the lab (60 h) and

6 colloquium (9 h)

Teaching Methods and Extent of Organized Teaching

Theoretical introduction to the lab (1.5 h)

10 days in the lab (60 h) and

6 colloquium (9 h)

Compulsory Assignments and Attendance

Compulsory participation in all experiments.

Forms of Assessment

Oral presentation of results and written lab report (composed of Introduction, Materials and Methods, Results, Discussion). Both have to be approved by the course leader. Assessment semester: Fall. 

Grading Scale

Character A-F.

Subject Overlap

BMED320 (-5 ECTS)

Assessment Semester

Fall

Reading List

To be announced (tba).

Course Evaluation

After every course.

Course Coordinator

From spring 2020: Karl A. Brokstad. E-mail: Karl.Brokstad@uib.no

Exam information

  • Type of assessment: Wtritten and oral examination

    Submission deadline
    04.07.2020, 23:59
    Withdrawal deadline
    01.02.2020
    Examination system
    Inspera
    Digital exam