Basic Course in Flow Cytometry
- ECTS credits5
- Teaching semesterSpring
- Course codeHUIMM307
- Number of semesters1
- LanguageEnglish
- Resources
Main content
ECTS Credits
5
Level of Study
Master students, PhD candidates
Teaching semester
Spring
There is no direct registration in this course, but each person that would like to attend has to contact Brith Bergum as there is a limited number of spaces.
Place of Instruction
Core facility for flow cytometry, Department of Clinical Science, Laboratory building 5th floor
Objectives and Content
Aim: Basic introduction to flow cytometry. The practical part of the course should enable the students to understand applications for flow cytometry. In addition, the applications used during the practical part of the course will be introduced in seminars by the course leaders.
Content:
Theoretical part:
-The course leaders will give a theoretical introduction to flow cytometry and some of the most common applications.
Practical part: Hands-on training at one of the local flow cytometers (BD AccuriC6)
- Quantification of apoptotic cells
- Cell cycle analyses
- Simple staining of cells with surface markers
- Analysis of transfected cells
Learning Outcomes
On completion of the course HUIMM307 the student is able to/has:
Knowledge
- understanding for basic applications for flow cytometry
- knowledge on which and how many different fluorochromes one can use on a given flow cytometer
- evaluate the quality of flow cytometry data
Skills
- plan and perform basic experiments using flow cytometry
- quality control generated flow cytometry data
- present flow cytometry data in a scientific way
Competence
- understanding of basic flow cytometry
- understanding as to why to use flow cytometry in a certain research question
- interpret basic flow cytometry data
Required Previous Knowledge
Basic knowledge on apoptosis and cell cycle.
Experience with basic laboratory techniques such as pipetting and centrifugation.
Recommended Previous Knowledge
Biology or molecular biology on bachelor level.
Access to the Course
The course will only be given if at least 4 students register for the course, and is limited to 10 students.
Course-coordinator decides who can participate.
Teaching and learning methods
23 x 1 hour lectures/seminars, 17 x 1 hour practical lab work
Teaching Methods and Extent of Organized Teaching
23 x 1 hour lectures/seminars, 17 x 1 hour practical lab work
Compulsory Assignments and Attendance
Compulsory participation in all experiments.
Forms of Assessment
Oral presentation of results and written lab report (composed of Introduction, Materials and Methods, Results, Discussion). Both have to be approved by the course leader.
Grading Scale
A-F
Assessment Semester
Spring
Because of lack of necessary equipment, the course must unfortunately be cancelled for Autumn 2021. We hope to be able to arrange the course Spring 2022.
Reading List
Flow cytometry - A Basic Introduction,
Wiki version; Michael Ormerod.
Course Evaluation
After every course
Course Administrator
Thea Midtun (thea.midtun@uib.no)
Department
Bergen Research School in Inflammation, Broegelmann Research Laboratory,
Department of Clinical Science.
Contact
Vår 2023: Jørn Skavland (jorn.skavland@uib.no)
Brith Bergum (brith.bergum@uib.no)
Exam information
Type of assessment: Report, assignment
- Submission deadline
- 13.04.2023, 23:59
- Withdrawal deadline
- 01.02.2023
- Examination system
- Inspera
- Digital exam
Type of assessment: Wtritten and oral examination
- Withdrawal deadline
- 01.02.2023
- Examination system
- Inspera
- Digital exam