Basic Course in Flow Cytometry

ECTS Credits

5

Level of Study

Master students, PhD candidates

Teaching semester

Spring

The course will only be given if at least 4 students register for the course, and is limited to 10 students.

Course coordinator decides who can participate.

Place of Instruction

Core facility for flow cytometry, Department of Clinical Science, Laboratory building 5th floor

Objectives and Content

Aim: Basic introduction to flow cytometry. The practical part of the course should enable the students to understand applications for flow cytometry. In addition, the applications used during the practical part of the course will be introduced in seminars by the course leaders.

Content:

Theoretical part:

-The course leaders will give a theoretical introduction to flow cytometry and some of the most common applications.

Practical part: Hands-on training at one of the local flow cytometers (BD AccuriC6)

- Quantification of apoptotic cells

- Cell cycle analyses

- Simple staining of cells with surface markers

- Analysis of transfected cells

Learning Outcomes

On completion of the course HUIMM307 the student is able to/has:

 Knowledge

- understanding for basic applications for flow cytometry

- knowledge on which and how many different fluorochromes one can use on a given flow cytometer

- evaluate the quality of flow cytometry data

Skills

- plan and perform basic experiments using flow cytometry

- quality control generated flow cytometry data

- present flow cytometry data in a scientific way

Competence

- understanding of basic flow cytometry

- understanding as to why to use flow cytometry in a certain research question

- interpret basic flow cytometry data

Required Previous Knowledge

Basic knowledge on apoptosis and cell cycle.

Experience with basic laboratory techniques such as pipetting and centrifugation.

Recommended Previous Knowledge

Biology or molecular biology on bachelor level.

Access to the Course

The course will only be given if at least 4 students register for the course, and is limited to 10 students.

Course-coordinator decides who can participate.

Teaching and learning methods

23 x 1 hour lectures/seminars, 17 x 1 hour practical lab work

Teaching Methods and Extent of Organized Teaching

23 x 1 hour lectures/seminars, 17 x 1 hour practical lab work

Compulsory Assignments and Attendance

Compulsory participation in all experiments.

Forms of Assessment

Oral presentation of results and written lab report (composed of Introduction, Materials and Methods, Results, Discussion). Both have to be approved by the course leader.

Grading Scale

A-F

Assessment Semester

Spring

Reading List

Flow cytometry - A Basic Introduction,

Wiki version; Michael Ormerod.

Course Evaluation

After every course

Course Coordinator

Brith Bergum (brith.bergum@uib.no)

Department

Bergen Research School in Inflammation, Broegelmann Research Laboratory,

Department of Clinical Science.