Experimental Molecular Biology II
Spring. This course has a limited capacity, enrolment is based on application. Application deadline is Thursday in week 2 for the spring semester.
Please see this page for more information: https://www.uib.no/en/matnat/53431/admission-courses-limited-capacity
You will receive confirmation of whether you received a spot in Studentweb no later than Tuesday the week after the deadline.
The first lecture/orientation meeting is compulsory, which means you will lose your spot if you do not attend class that day. The time of the first lecture/orientation meeting can be found in the schedule on the course website or on the "Mitt UiB" learning platform.
Objectives and Content
This course builds on MOL221, Experimental molecular biology I. In MOL222 students will follow laboratory protocols to perform molecular cloning of two protein-coding genes followed by their mammalian cell expression and analysis using immuno-detection and microscopy techniques. The results obtained are reported in a scientific publication (IMRaD) format which is evaluated.
In MOL222, the students will get theoretical and practical introduction to important methods and techniques in biochemistry and molecular biology. These include molecular cloning, PCR, plasmid propagation in bacteria, plasmid purification, restriction analysis, overexpression of genes in mammalian cell lines, microscopy, as well as gel electrophoresis and Western blotting. Furthermore, students will get hands-on experience in the usage of bioinformatics tools for molecular cloning and will learn the physical and chemical basis of the related analytical methods.
Along with the practical aspects of the course, particular emphasis will be made on the development of scientific communication skills including proper documentation of the experimental procedures, experimental results, background information and critical evaluation of the obtained results including their quantitative analysis. Laboratory safety aspects will also be a focus.
The purpose of the course is to provide solid basic knowledge and skills in experimental molecular biology techniques and in scientific communication thus establishing the basis for further studies in molecular biology.
On completion of the course the student should have the following learning outcomes defined in terms of knowledge, skills and general competence:
- can explain the principles of molecular cloning and PCR, cell transfection and Western blotting
- knows the structure of a scientific report and the way experimental results are presented in a scientific communication
- knows the general safety routines for laboratory work in molecular biology
- can prepare plasmids for transfection of cultured cells and analyse protein expression by means of fluorescence microscopy and Western blotting
- can use instrumentation and gene technology methods for separation and analysis of proteins and nucleic acids
- can interpret and report data both qualitatively and quantitatively
- can follow general safety routines for laboratory work in molecular biology
- can plan experimental work based on a protocol
- can critically evaluate and discuss experimental results
- can critically evaluate and correctly cite literature
- can write scientifically
- can apply protocols for basic experimental work within the field of molecular biology and biochemistry
- can properly communicate experimental procedures as well as critically evaluate and discuss obtained experimental results within the field of molecular biology and biochemistry
Required Previous Knowledgewww.uib.no/en/course/MOL100
MOL221 must be completed successfully or taken during the same semester as MOL222. Courses other than MOL221, with a similar scope, can be considered, if successfully completed and, if approved, can replace MOL221 as requirement.
Credit Reduction due to Course Overlap
MOL202: 10 sp.
Compulsory Assignments and Attendance
All activities are mandatory including the introductory meeting, lectures and lab exercises. Completed activities are valid for 6 semesters.
Forms of Assessment
One written lab report for all the individual lab projects.
Examination Support Material
The grading scale used is A to F. Grade A is the highest passing grade in the grading scale, grade F is a fail.
Spring semester only.
Department og Biological Sciences, e-mail: firstname.lastname@example.org