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Core Facility for Metabolomics

The instrument

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Picture 1. Ersilia Bifulco and LC-MS/MS instrumentation.
Picture 1. Ersilia Bifulco and LC-MS/MS instrumentation.
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Illustration photo of Kai Triebner and the pipetting robot
Picture 2. Kai Triebner and the pipetting robot.
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Two liquid chromatography-tandem mass spectrometry (LC-MS/MS) units from Waters are used to analyze samples (Picture 1). The instruments have an upper mass range limit of approximately 2000 Da, and mass resolution is slightly better than 1 Da. They are state of the art with regard to analytical sensitivity. Section engineer Ersilia Bifulco is one of two engineers who are attached to the core facility. She holds a doctorate in mass spectrometry from Università degli Studi di Cagliari (Italy).

Most sample processing is robotized and carried out on a Hamilton STAR robot (Picture 2). The instrument has 8 channels and pipettes volumes of 0.5-1 000 µL precisely and accurately. PhD student Kai Triebner has programmed the robot. He is Diplomingenieur from Technische Universität Dresden (Germany).

The laboratory is equipped with a nitrogen evaporator, centrifuge and freezers.

The core facility is headed by professor Steinar Hustad, who is affiliated to the Department of Clinical Science. He is a specialist in endocrinology and internal medicine.