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Anne Marie Simonne Døskeland's picture

Anne Marie Simonne Døskeland

Senior Engineer
  • E-mailAnne.Doskeland@uib.no
  • Phone+47 55 58 64 56
  • Visitor Address
    Jonas Lies vei 91
  • Postal Address
    Postboks 7804
    5020 Bergen

Dr. Anne P. Døskeland (Cand real, PhD) is employed at the Proteomics Unit at the University of Bergen Master Degrees in Chemistry and Medical Sciences (1973) were obtained at the Sciences and Medical Faculties of the University of Louvain, Belgia. PhD (1991) was obtained at the Institute of Biochemistry, University of Bergen, Norway.

Anne is affiliated to the facility service PROBE and responsible for the mass spectrometric analysis of samples delivered by clients. Most of studies consist in identification of proteins by MALDI-TOF-TOF or LCMSMS.

Her main research area is related to the regulation of the ubiquitin-proteasome pathway.

Induction of apoptosis by proteasome inhibitors (bortezomib, PR171) has been characterized in leukemia cells, and a proteome/degradome profiling of proteins affected by drugs bortezomib and anthracyclin is studied using mass spectrometric methods. Prof. J. Vandekerckhove has contributed to the work with its expertise in Cofradic methodology.

Journal articles
  • Berge, Tone; Eriksson, Anna; Brorson, Ina Skaara; Høgestøl, Einar August; Berg-Hansen, Pål; Døskeland, Anne Marie Simonne; Mjaavatten, Olav; Bos, Steffan Daniel; Harbo, Hanne Flinstad; Berven, Frode. 2019. Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls. Clinical Proteomics. 16:19: 1-18. doi: 10.1186/s12014-019-9241-5
  • Johansson, Bente Berg; Fjeld, Karianne; Solheim, Marie Holm; Shirakawa, Jun; Zhang, Enming; Keindl, Magdalena; Hu, Jiang; Lindqvist, Andreas; Døskeland, Anne Marie Simonne; Mellgren, Gunnar; Flatmark, Torgeir; Njølstad, Pål Rasmus; Kulkarni, Rohit N.; Wierup, Nils; Aukrust, Ingvild; Bjørkhaug, Lise. 2017. Nuclear import of glucokinase in pancreatic beta-cells is mediated by a nuclear localization signal and modulated by SUMOylation. Molecular and Cellular Endocrinology. 454: 146-157. doi: 10.1016/j.mce.2017.06.020
  • Yadetie, Fekadu; Oveland, Eystein; Døskeland, Anne Marie Simonne; Berven, Frode; Goksøyr, Anders; Karlsen, Odd Andre. 2017. Quantitative proteomics analysis reveals perturbation of lipid metabolic pathways in the liver of Atlantic cod (Gadus morhua) treated with PCB 153. Aquatic Toxicology. 185: 19-28. doi: 10.1016/j.aquatox.2017.01.014
  • Døskeland, Anne Marie Simonne. 2015. Release and bioactivity of bone morphogenetic protein-2 are affected by scaffold binding techniques in vitro and in vivo. Journal of Controlled Release. 197: 148-157. doi: 10.1016/j.jconrel.2014.11.003.Epub2014Nov10
  • Suliman, Salwa; Xing, Zhe; Wu, X; Xue, Ying; Pedersen, Torbjørn Østvik; Sun, Y; Døskeland, Anne Marie Simonne; Nickel, J; Waag, T.; Lygre, Henning; Finne-Wistrand, Anna; Steinmüller-Nethl, Doris; Krueger, A; Mustafa, Kamal Babikeir Eln. 2015. Release and bioactivity of bone morphogenetic protein-2 are affected by scaffold binding techniques in vitro and in vivo. Journal of Controlled Release. 197: 148-157. doi: 10.1016/j.jconrel.2014.11.003
  • Calvo, Ana C; Pey, Angel L.; Miranda-Vizuete, Antonio; Døskeland, Anne Paulus; Martinez, Aurora. 2011. Divergence in enzyme regulation between Caenorhabditis elegans and human tyrosine hydroxylase, the key enzyme in the synthesis of dopamine. Biochemical Journal. 434: 133-141. doi: 10.1042/BJ20101561
  • Stapnes, Camilla I; Døskeland, Anne Paulus; Hatfield, Kimberley Joanne; Ersvær, Elisabeth; Ryningen, Anita; Lorens, James; Gjertsen, Bjørn Tore; Bruserud, Øystein. 2007. The proteasome inhibitors bortezomib and PR-171 have antiproliferative and proapoptotic effects on primary human acute myeloid leukaemia cells. British Journal of Haematology. 136: 814-828. doi: 10.1111/j.1365-2141.2007.06504.x
  • Døskeland, Anne Paulus. 2006. A method for simple identification of signature peptides derived from polyUb-K48 and K63 by MALDI-TOF MS and chemically assisted MS/MS fragmentation. Amino Acids. 30: 99-103. doi: 10.1007/s00726-005-0227-4
  • Sjøholt, Gry; Bedringaas, Siv Lise; Døskeland, Anne Paulus; Gjertsen, Bjørn Tore. 2006. Proteomic strategies for individualizing therapy of acute myeloid leukemia (AML). Current Pharmaceutical Biotechnology. 7: 159-170. doi: 10.2174/138920106777549759
  • Sjøholt, Gry; Bedringaas, Siv Lise; Døskeland, Anne Paulus; Gjertsen, Bjørn Tore. 2006. Proteomic strategies for individualizing therapy of acute myeloid leukemia (AML). Current Pharmaceutical Biotechnology. 7: 159-170. doi: 10.2174/138920106777549759
  • Lauvrak, Silje Anett Ugland; Hollås, Hanne; Døskeland, Anne Paulus; Aukrust, Ingvild; Flatmark, Torgeir; Vedeler, Anni. 2005. Ubiquitinated annexin A2 is enriched in the cytoskeleton fraction. FEBS Letters. 579: 203-206. doi: 10.1016/j.febslet.2004.11.076
  • Døskeland, Anne Paulus; Flatmark, Torgeir. 2002. Ubiquitination of soluble and membrane-bound tyrosine hydroxylase and degradation of the soluble form. European Journal of Biochemistry (EJB). 269: 1561-1569.
  • Thorolfsson, Matthias; Døskeland, Anne Paulus; Muga, Arturo; Martinez, Aurora. 2002. The binding of tyrosine hydroxylase to negatively charged lipid bilayers involves the N-terminal region of the enzyme. FEBS Letters. 519: 221-226.
  • Døskeland, Anne Paulus; Flatmark, Torgeir. 2001. Conjugation of phenylalanine hydroxylase with polyubiquitin chains catalysed by rat liver enzymes. Biochimica et Biophysica Acta. 1547: 379-386.
  • Flatmark, Torgeir; Erlandsen, Heidi; Bjørgo, Elisa; Solstad, Therese; Døskeland, Anne Paulus; Stevens, Raymond C. 2000. Regulatory properties of tetrahydrobiopterin cofactor bound at the active site of phenylalanine hydroxylase. Pteridines. 11: 34-36.
  • Hovland, Randi; Døskeland, Anne Paulus; Eikhom, Thor S; Robaye, B; Døskeland, Stein Ove. 1999. cAMP induces co-translational modification of proteins in IPC-81 cells. Biochemical Journal. 342: 369-377.
  • Døskeland, Anne Paulus; Flatmark, Torgeir. 1996. Recombinant human phenylalanine hydroxylase is a substrate for the ubiquitin conjugating enzyme system. Biochemical Journal. 319: 941-945.
  • Døskeland, Anne Paulus; Martinez, Aurora; Knappskog, Per; Flatmark, Torgeir. 1996. Phosphorylation of recombinant human phenylalanine hydroxylase. Effect on catalytic activity, substrate activation and protection against non-spesific cleavage of the fusion protein by restriction protease. The Biochemical journal. 319: 409-414.
  • Døskeland, Anne Paulus; Martinez, Aurora; Knappskog, Per; Flatmark, Torgeir. 1996. Phosphorylation of recombinant human phenylalanine hydroxylase. Effect on catalytic activity, substrate activation and protection against non-spesific cleavage of the fusion protein by restriction protease. The Biochemical journal. 319: 409-414.
  • Døskeland, Anne Paulus; Martinez, Aurora; Knappskog, Per; Flatmark, Torgeir. 1996. Phosphorylation of recombinant human phenylalanine hydroxylase. Effect on catalytic activity, substrate activation and protection against non-specific cleavage of the fusion protein by restriction protease. Biochemical Journal. 319: 409-414.
  • Robay, B.; Døskeland, Anne Paulus; Riviera-Lopez, S.; Døskeland, Stein Ove; Dumont, J. 1994. Apoptotic Cell Death analyzed at the molecular level by two-dimensional gel electrophoresis. Electrophoresis. 15: 503-510.
  • Døskeland, Anne Paulus; Vintermyr, Olav Karsten; Flatmark, Torgeir; Cotton, R. G. H.; Døskeland, Stein Ove. 1992. Phenylalanine modulates differently the phosphorylation of phenylalanine 4-monooxygenase in intact rat hepatocytes exposed to cAMP and Ca2+-elevating agents. European Journal of Biochemistry (EJB). 206: 161-170.
  • Døskeland, Anne Paulus; Vintermyr, Olav Karsten; Flatmark, Torgeir; Cotton, R. G. H.; Døskeland, Stein Ove. 1992. Phenylatanine modulates positively the cAMP-dependant phosphorylation and modulates negatively the vasopressin-and okadeic acid-induced phosphorylation of phenylalanine 4-monoxygenase in intact rat hepatocytes. European Journal of Biochemistry (EJB). 206: 161-170.
Reports and theses
  • Døskeland, Anne Paulus. 1991. Regulatory properties of phenylalanine 4-monooxygenase (phenylalanine hydroxylase). [Mangler utgivernavn], Bergen Universitetet i Bergen.
Book sections
  • Flatmark, Torgeir; Almås, Bjørg; Døskeland, Anne Paulus. 2002. L-DOPA responsive dystonia/infantile parkinsonism associated with mutations at the human tyrosine hydroxylase locus. 471-474. In:
    • 2002. In “Catecholamine Research: From Molecular Insights to Clinical Medicine”.

More information in national current research information system (CRIStin)

Publications in PubMed

Studied subjects related to the Ubiquitin-proteasome pathway

  1. Conjugation of ubiquitin to substrates phenylalanine and tyrosine hydroxylases, and their degradation by the proteasome
  2. Mass spectrometric analysis by MALDI of polyubiquitin chains
  3. The Ub-proteasome pathway regulation: its inhibition by bortezomib and PR171 in leukemia cells, and MS/MS analysis of the degradome in order to define the proteins affected by the drugs in the apoptotic process.

Research groups