Tissue microenvironment: interstitial fluid in skin, muscle and tumor
During the first years I worked with development of techniques for measurement of interstitial fluid pressure, primarily in skin and muscle. I showed that the servocontrolled micropipette technique could be used for measurement of interstitial fluid pressure (Pif), and also worked with other techniques for Pif measurements. As one of the first I measured Pif in tumors and found that these pressures were markedly elevated that has later been verified in numerous studies.
I also established techniques to measure compliance (stiffness, yielding in properties in tissues, of importance for edema prevention) and performed several studies addressing this topic. I have also contributed and am part of a team that has studied the mechanistic role of integrins in control of Pif.
Another focus of my research has been the interstitial fluid, i.e. the fluid bathing the cells of the interstitial matrix. This fluid is not readily accessible, and I have been working to establish methods for interstitial fluid isolation. Of major interest is isolation of such fluid in tumors, and I recently showed that exposing tumors to an increased G-force could result in isolation of such fluid.
Another area of interest is the effect of the extracellular matrix on distribution of extracellular proteins in the interstitial fluid (exclusion), and I have been able to quantify such effect. Specifically, I have been able to separate the steric and charge effect of exclusion, and also to relate this to tissue hydration. This phenomenon is of importance for plasma volume regulation.
Recently I have become interested in the role of the lymphatics in fluid volume regulation and also in relation to tumor metastasis.
- 2017. Interstitial IgG antibody pharmacokinetics assessed by combined in vivo- and physiologically-based pharmacokinetic modelling approaches. Journal of Physiology. 595: 7311-7330. doi: 10.1113/JP274819
- 2017. GRHL2 Is Required for Collecting Duct Epithelial Barrier Function and Renal Osmoregulation. Journal of the American Society of Nephrology. doi: 10.1681/ASN.2017030353
- 2017. Fluid accumulation after closure of atrial septal defects: the role of colloid osmotic pressure. Interactive Cardiovascular and Thoracic Surgery. 26: 307-312. doi: 10.1093/icvts/ivx334
- 2017. Lymphangiogenesis Facilitates Initial Lymph Formation and Enhances the Dendritic Cell Mobilizing Chemokine CCL21 Without Affecting Migration. Arteriosclerosis, Thrombosis and Vascular Biology. 37: 2128-2135. doi: 10.1161/ATVBAHA.117.309883
- 2017. The notochord in Atlantic salmon (Salmo salar L.) undergoes profound morphological and mechanical changes during development. Journal of Anatomy. 231: 639-654. doi: 10.1111/joa.12679
- 2017. High-salt diet causes osmotic gradients and hyperosmolality in skin without affecting interstitial fluid and lymph. Hypertension. 69: 660-668. doi: 10.1161/HYPERTENSIONAHA.116.08539
- 2017. Role of Hyperplasia of Gingival Lymphatics in Periodontal Inflammation. Journal of Dental Research. 96: 467-476. doi: 10.1177/0022034516681762
- 2017. The interstitium conducts extrarenal storage of sodium and represents a third compartment essential for extracellular volume and blood pressure homeostasis. Acta Physiologica. doi: 10.1111/apha.13006
- 2016. Lymphatic vessels regulate immune microenvironments in human and murine melanoma. Journal of Clinical Investigation. 126: 3389-3402. doi: 10.1172/JCI79434
- 2016. Electrostatic, elastic and hydration-dependent interactions in dermis influencing volume exclusion and macromolecular transport. Journal of Theoretical Biology. 400: 80-91. doi: 10.1016/j.jtbi.2016.03.044
- 1986 PhD, University of Bergen: Bergen, Norway
- 1974-1981, Medical Doctor (MD) University of Bergen: Bergen, Norway