Tissue microenvironment: interstitial fluid in skin, muscle and tumor
During the first years I worked with development of techniques for measurement of interstitial fluid pressure, primarily in skin and muscle. I showed that the servocontrolled micropipette technique could be used for measurement of interstitial fluid pressure (Pif), and also worked with other techniques for Pif measurements. As one of the first I measured Pif in tumors and found that these pressures were markedly elevated that has later been verified in numerous studies.
I also established techniques to measure compliance (stiffness, yielding in properties in tissues, of importance for edema prevention) and performed several studies addressing this topic. I have also contributed and am part of a team that has studied the mechanistic role of integrins in control of Pif.
Another focus of my research has been the interstitial fluid, i.e. the fluid bathing the cells of the interstitial matrix. This fluid is not readily accessible, and I have been working to establish methods for interstitial fluid isolation. Of major interest is isolation of such fluid in tumors, and I recently showed that exposing tumors to an increased G-force could result in isolation of such fluid.
Another area of interest is the effect of the extracellular matrix on distribution of extracellular proteins in the interstitial fluid (exclusion), and I have been able to quantify such effect. Specifically, I have been able to separate the steric and charge effect of exclusion, and also to relate this to tissue hydration. This phenomenon is of importance for plasma volume regulation.
Recently I have become interested in the role of the lymphatics in fluid volume regulation and also in relation to tumor metastasis.
- (2023). VEGF-B hypertrophy predisposes to transition from diastolic to systolic heart failure in hypertensive rats. Cardiovascular Research (CVR). 1553-1567.
- (2023). Isolation of lymph shows dysregulation of STAT3 and CREB pathways in the spleen and liver during leukemia development in a rat model. Microcirculation.
- (2022). Na+ is shifted from the extracellular to the intracellular compartment and is not inactivated by glycosaminoglycans during high salt conditions in rats. Journal of Physiology. 2293-2309.
- (2022). Lymphatics in Malignant Tumors. 10 pages.
- (2022). Genetic engineering of lymphangiogenesis in skin does not affect blood pressure in mouse models of salt-sensitive hypertension. Hypertension. 2451-2462.
- (2021). The Gut Microbiome in Hypertension: Recent Advances and Future Perspectives. Circulation Research. 934-950.
- (2021). Skin Sodium Accumulates in Psoriasis and Reflects Disease Severity. Journal of Investigative Dermatology. 166-178.
- (2021). Pharmacokinetics and pharmacodynamics of t-cell bispecifics in the tumour interstitial fluid. Pharmaceutics.
- (2021). As for blood vessels, the answer regarding lymphatics is often NO. Acta Physiologica.
- (2021). Abnormal neonatal sodium handling in skin precedes hypertension in the SAME rat. Pflügers Archiv: European Journal of Physiology.
- 1986 PhD, University of Bergen: Bergen, Norway
- 1974-1981, Medical Doctor (MD) University of Bergen: Bergen, Norway