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Nils-Kåre Birkeland's picture

Nils-Kåre Birkeland

Professor
  • E-mailNils.Birkeland@uib.no
  • Phone+47 55 58 26 57
  • Visitor Address
    Thormøhlensgt. 53 A/B
    Room 
    3G20
  • Postal Address
    Postboks 7803
    5020 Bergen

My interests include environmental and molecular microbiology, and evolution and adaptations of prokaryotes. Current research activities involve thermophilic bacteria and archaea, metagenomics in extreme environments, extremophilic methane oxidition, and enteropathogenic bacteria in tropical fresh-waters. Present work aims at (1) identifying molecular adaptations to extreme temperatures using extremophilic microorganisms from a variety of environments, (2) enhance our understanding of the enourmous natural microbial diversity in extreme habitats, (3) elucidation of mechanisms and regulation of biological methane oxidation, (4) metagenomics, and (5) to obtain knowledge about the survival and evolution of Shigella-like bacteria. 

Academic article
  • 2020. A novel cold-adapted Methylovulum species, with a high C16:1ω5c content, isolated from an arctic thermal spring in spitsbergen. Microbes and Environments. 1-9.
  • 2019. Insights into the Bacterial Diversity of the Acidic Akhtala Mine Tailing in Armenia Using Molecular Approaches. Current Microbiology. 462-469.
  • 2019. Global Biogeographic Distribution Patterns of Thermoacidophilic Verrucomicrobia Methanotrophs Suggest Allopatric Evolution. Frontiers in Microbiology. 1-11.
  • 2019. Complete genome sequence analysis of the thermoacidophilic verrucomicrobial methanotroph “Candidatus Methylacidiphilum kamchatkense” strain Kam1 and comparison with its closest relatives. BMC Genomics. 1-15.
  • 2019. Bacterial Community Profiling of Tropical Freshwaters in Bangladesh. Frontiers In Public Health. 1-16.
  • 2019. A glimpse of the prokaryotic diversity of the Large Aral Sea reveals novel extremophilic bacterial and archaeal groups. MicrobiologyOpen. 1-13.
  • 2018. Bacilli community of saline–alkaline soils from the Ararat Plain (Armenia) assessed by molecular and culture-based methods. Systematic and Applied Microbiology. 232-240.
  • 2017. Protection against shigellosis caused by shigella dysenteriae serotype 4 in Guinea pigs using escherichia albertii dm104 as a live vaccine candidate strain. Acta Microbiologica et Immunologica Hungarica. 151-164.
  • 2017. Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island. Extremophiles. 733-742.
  • 2017. Complete genome sequence analysis of Archaeoglobus fulgidus strain 7324 (DSM 8774), a hyperthermophilic archaeal sulfate reducer from a North Sea oil field. Standards in Genomic Sciences.
  • 2016. Acid-tolerant moderately thermophilic methanotrophs of the class Gammaproteobacteria isolated from tropical topsoil with methane seeps. Frontiers in Microbiology.
  • 2015. Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats. Microorganisms.
  • 2015. Evidence for extensive gene flow and Thermotoga subpopulations in subsurface and marine environments. The ISME Journal. 1532-1542.
  • 2014. Microbial diversity in an Armenian geothermal spring assessed by molecular and culture-based methods. Journal of Basic Microbiology. 1240-1250.
  • 2014. Microbial community analysis of thermophilic mixed culture sludge for biohydrogen production from palm oil mill effluent. International journal of hydrogen energy. 19285-19293.
  • 2014. An Environmental escherichia albertii strain, dm104, induces protective immunity to shigella dysenteriae in guinea pig eye model. Current Microbiology. 642-647.
  • 2014. Activation of the MCM helicase from the thermophilic archaeon, Thermoplasma acidophilum by interactions with GINS and Cdc6-2. Extremophiles. 915-924.
  • 2013. Thermoanaerobacter spp. recovered from hot produced water from the Thar Jath oil-field in South Sudan. African Journal of Microbiology Research. 5219-5226.
  • 2013. Serological cross-reaction between O-antigens of Shigella dysenteriae type 4 and an environmental Escherichia albertii isolate. Current Microbiology. 590-595.
  • 2013. Prevalence and distribution of different diarrhoeagenic Escherichia coli virulotypes in major water bodies in Bangladesh. Epidemiology and Infection. 2516-2525.
  • 2013. Heavy metal accumulation and the expression of the copA and nikA genes in Bacillus subtilis AG4 isolated from the Sotk gold mine in Armenia. Biological Journal of Armenia. 51-57.
  • 2013. Evidence for successional development in Antarctic hypolithic bacterial communities. The ISME Journal. 2080-2090.
  • 2012. Prevalence of a novel division-level bacterial lineage in Lake Dhanmondi, Dhaka, Bangladesh, as revealed by deep sequencing of 16S rRNA gene amplicons. Current Microbiology. 356-360.
  • 2012. Evidence of interspecies O antigen gene cluster transfer between Shigella boydii 15 and Escherichia fergusonii. Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS). 959-966.
  • 2012. Differential expression of particulate methane monooxygenase genes in the verrucomicrobial methanotroph 'Methylacidiphilum kamchatkense' Kam1. Extremophiles. 405-409.
  • 2011. Uracil-DNA Glycosylase of Thermoplasma acidophilum Directs Long-Patch Base Excision Repair, Which Is Promoted by Deoxynucleoside Triphosphates and ATP/ADP, into Short-Patch Repair. Journal of Bacteriology. 4495-4508.
  • 2011. The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication. Extremophiles. 529-539.
  • 2011. Recovery and Characterization of Environmental Variants of Shigella flexneri from Surface Water in Bangladesh. Current Microbiology. 372-376.
  • 2010. Virulence of environmental Stenotrophomonas maltophila serologically cross-reacting with Shigella-specific antisera. Pakistan Journal of Biological Sciences. 937-945.
  • 2010. Thermodynamic and kinetic stability of a large multi-domain enzyme from the hyperthermophile Aeropyrum pernix. Extremophiles. 213-223.
  • 2010. The Hyperthermophilic Euryarchaeon Archaeoglobus fulgidus Repairs Uracil by Single-Nucleotide Replacement. Journal of Bacteriology. 5755-5766.
  • 2010. Survival study of Eschericia coli O157:H7 in the aquatic system of Bangladesh. Dhaka University Journal of Biological Sciences. 195-201.
  • 2010. Intracellular Oceanospirillales bacteria inhabit gills of Acesta bivalves. FEMS Microbiology Ecology. 523-533.
  • 2009. The Genome of Thermosipho africanus TCF52B: Lateral Genetic Connections to the Firmicutes and Archaea. Journal of Bacteriology. 1974-1978.
  • 2009. SulfoSYS (Sulfolobus Systems Biology): towards a silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation. Biochemical Society Transactions. 58-64.
  • 2009. SulfoSYS (Sulfolobus Systems Biology): towards a silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation. Biochemical Society Transactions. 58-64.
  • 2009. Prevalence of antimicrobial resistant bacteria cultured from cattle feces and surface water. Bangladesh Journal of Zoology. 147-156.
  • 2009. Optimization and microbial community analysis for production of biohydrogen from palm oil mill effluent by thermophilic fermentative process. International journal of hydrogen energy. 7448-7459.
  • 2009. Kosmotoga olearia gen. nov., sp nov., a thermophilic, anaerobic heterotroph isolated from an oil production fluid. International Journal of Systematic and Evolutionary Microbiology. 2991 -3000.
  • 2009. Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis. Bioresource Technology. 909-918.
  • 2009. Comparison of Cultural and Molecular Characteristics of Escherichia coli isolated from Freshwater of Bangladesh. Dhaka University Journal of Biological Sciences. 195-199.
  • 2009. Biochemical characterization of the minichromosome maintenance protein from the archaeon Thermoplasma acidophilum. Extremophiles. 81-88.
  • 2008. Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity. Nucleic Acids Research. 5602-5609.
  • 2008. Optimization of simultaneous thermophilic fermentative hydrogen production and COD reduction from palm oil mill effluent by Thermoanaerobacterium-rich sludge. International journal of hydrogen energy. 1221-1231.
  • 2008. Microbial community structure analysis of produced water from a high-temperature North Sea oil-field. Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology. 37-49.
  • 2008. Methane oxidation at 55 degrees C and pH 2 by a thermoacidophilic bacterium belonging to the Verrucomicrobia phylum. Proceedings of the National Academy of Sciences of the United States of America. 300-304.
  • 2008. Methane assimilation and trophic interactions with marine Methylomicrobium in deep-water coral reef sediment off the coast of Norway. FEMS Microbiology Ecology. 320-330.
  • 2008. Insight into the microbial community structure of a Norwegian deep-water coral reef environment. Deep Sea Research Part I: Oceanographic Research Papers. 1554-1563.
  • 2008. Distribution of Crenarchaeota Representatives in Terrestrial Hot Springs of Russia and Iceland. Applied and Environmental Microbiology. 7620-7628.
  • 2008. Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum. Open Biochemistry Journal. 129-134.
  • 2008. Caldicellulosiruptor kronotskyensis sp nov and Caldicellulosiruptor hydrothermalis sp nov., two extremely thermophilic, cellulolytic, anaerobic bacteria from Kamchatka thermal springs. International Journal of Systematic and Evolutionary Microbiology. 1492-1496.
  • 2007. Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers. Extremophiles.
  • 2007. Thermal stability and biochemical properties of isocitrate dehydrogenase from the thermoacidophilic archaeon Thermoplasma acidophilum. Extremophiles. 397-402.
  • 2007. Structural basis for enzymatic excision of N-1-methyladenine and N-3-methylcytosine from DNA. EMBO Journal.
  • 2007. Structural basis for enzymatic excision of N-1-methyladenine and N-3-methylcytosine from DNA. EMBO Journal.
  • 2007. Serological cross-reactivity of environmental isolates of Enterobacter, Escherichia, Stenotrophomonas, and Aerococcus with Shigella spp.-specific antisera. Current Microbiology. 63-67.
  • 2007. Improvement of biohydrogen production and treatment etticiency on palm oil mill effluent with nutrient supplementation at thermophilic condition using an anaerobic sequencing batch reactor. Enzyme and Microbial Technology. 583-590.
  • 2007. Biochemical characterization of isocitrate dehydrogenase from Methylococcus capsulatus reveals a unique NAD(+)-dependent homotetrameric enzyme. Archives of Microbiology.
  • 2006. Thermovirga lienii gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, amino-acid-degrading bacterium isolated from a North Sea oil well. International Journal of Systematic and Evolutionary Microbiology. 1539-1545.
  • 2006. The crystal structure of a hyperthermostable subfamily II isocitrate dehydrogenase from Thermotoga maritima. The FEBS Journal. 2851-2868.
  • 2006. Tepidimicrobium ferriphilum gen nov, sp nov, a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales. International Journal of Systematic and Evolutionary Microbiology. 369-372.
  • 2006. Stimulation of MCM helicase activity by a Cdc6 protein in the archaeon Thermoplasma acidophilum. Nucleic Acids Research. 6337-6344.
  • 2006. Mineralization of 4-aminobenzenesulfonate (4-ABS) by Agrobacterium sp. strain PNS-1. Biodegradation. 495-502.
  • 2005. Transient accumulation of metabolic intermediates of p-cresol in the culture medium by a Pseudomonas sp strain A isolated from a sewage treatment plant. World Journal of Microbiology & Biotechnology. 1529-1534.
  • 2005. Reduction of uranium(VI) phosphate during growth of the thermophilic bacterium Thermoterrabacterium ferrireducens. Applied and Environmental Microbiology. 6423-6426.
  • 2005. Isocitrate dehydrogenase from the hyperthermophile Aeropyrum pernix: X-ray structure analysis of a ternary enzyme-substrate complex and thermal stability. Journal of Molecular Biology. 559-577.
  • 2004. The microbial diversity of deep subsurface oil reservoirs. Studies in Surface Science and Catalysis. 385-403.
  • 2004. The 2.9 Ångström resolution crystal structure of malate dehydrogenase from Archaeoglobus fulgidus: mechanisms of oligomerisation and thermal stabilisation. Journal of Molecular Biology. 343-356.
  • 2004. Genomic insights into methanotrophy: The complete genome sequence of Methylococcus capsulatus (Bath). PLoS Biology. 1616-1628.
  • 2002. Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus. Biochemistry. 12697-12705.
  • 2002. Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus. Biochemistry. 12697-12705.
  • 2002. Identification of cofactor discrimination sites in NAD-isocitrate dehydrogenase from Pyrococcus furiosus. Archives of Microbiology. 297-300.
  • 2002. Identification of cofactor discrimination siten in NAD-isocitrate dehydrogenase from Pyrococcus furious. Archives of Microbiology. 297-300.
  • 2002. Crystallization and preliminary X-ray structure analysis of isocitrate dehydrogenase from two hyperthermophiles, Aeropyrum pernix and Thermotoga maritima. Acta Crystallographica Section D: Biological Crystallography. 2162-2164.
  • 2002. Chromosome replication patterns in the hyperthermophilic euryarchaea Archaeoglobus fulgidus and Methanocaldococcus (Methanococcus) jannaschii. Molecular Microbiology. 1443-1450.
  • 2001. Excision of uracil from DNA by the hyperthermophilic Afung protein is dependent on the opposite base and stimulated by heat-induced transition to a more open structure. Mutation Research (DNA Repair). 175-192.
  • 2001. Differences in the oligomeric state of the LDH-like L-MalDH from the hyperthermophilic archaea Methanococcus jannaschii and Archaeoglobus fulgidus. Biochemistry. 10310-10316.
  • 2001. Differences in Oligomeric States of the (LDH-like)L-MalDH from the Hyperthermophilic Archaea Methanococcus jannaschii and Archaeoglobus fulgidus. Biochemistry. 10310-10316.
  • 2001. Comparison of isocitrate dehydrogenase from three different hyperthermophiles reveals differences in thermostability, cofactor specificity, oligomeric state and phylogenetic affiliation. Journal of Biological Chemistry. 43924-43931.
  • 2001. Comparison of Isocitrate Dehydrogenase from Three Hyperthermophiles Reveals Differences in Thermostability,Cofactor Specificity,Oligomeric State, and Phylogenetic Affiliation. Journal of Biological Chemistry. 43924-43931.
  • 2001. Archaeoglobus fulgidus isocitrate dehydrogenase, malate dehydrogenase and glutamate dehydrogenase. Methods in Enzymology. 13-26.
  • 2001. A novel organization of the dissimilatory sulfite reductase operon of Thermodesulforhabdus norvegica verified by RT-PCR. FEMS Microbiology Letters. 81-85.
  • 2000. Characterization of the desulforubidin operons from Desulfobacter vibrioformis and Desulfobulbus rhabdoformis. FEMS Microbiology Letters. 41-46.
  • 1999. Dissimilatory sulfite reductase from <I>Archaeoglobus profundus</I> and <I>Desulfotomaculum thermocisternum</I>: phylogenetic and structural implications from gene sequences. Extremophiles. 63-70.
  • 1998. Purification and characterization of a monomeric isocitrate dehydrogenase from the sulfate-reducing bacterium Desulfobacter vibrioformis and demonstration of the presence of a monomeric enzyme in other bacteria. FEMS Microbiology Letters. 469-474.
  • 1998. Petrotoga mobilis sp. nov., from a North Sea oil-production well. International Journal of Systematic Bacteriology. 1007-1013.
  • 1998. Petrotoga mobilis sp. nov., from a North Sea oil-production well. International Journal of Systematic Bacteriology. 1007-1014.
  • 1998. Characterization and sequence comparison of temperature-regulated chaperonins from the hyperthermophilic archaeon <I>Archaeoglobus fulgidus</I>. Gene. 431-438.
  • 1997. Purification and properties of an extremely thermostable NADP-specific glutamate dehydrogenase from Archaeoglobus fulgidus. Archives of Microbiology. 536-539.
  • 1997. Properties and primary structure of a thermostable L-malate dehydrogenase from Archaeoglobus fulgidus. Archives of Microbiology. 59-67.
  • 1997. Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, Archaeoglobus fulgidus. Archives of Microbiology. 412-420.
  • 1994. Cloning, molecular characterization, and expression of the genes encoding the lytic functions of lactococcal bacteriophage phi-LC3: a dual lysis system og modular design. Canadian Journal of Microbiology (print). 658-665.
  • 1993. Characterization of Genetic Elements Required for Site-Specific Integration of the Temperate Lactococcal Bacteriophage φLC3 and Construction of Integration-Negative φLC3 Mutants. Journal of Bacteriology. 1745-1755.
Lecture
  • 2015. Methane oxidation by extremophilic verrucomicrobia.
  • 2015. Cdc45-independent activation of replicative helicase in the thermophilic archaeon, Thermoplasma acidophilum.
  • 2014. Archaea; The third domain of life. Diversity and temperature adaptations.
Academic lecture
  • 2019. Complete genome sequence analysis of the thermoacidophilic verrucomicrobial methanotroph “Ca. Methylacidiphilum kamchatkense” strain Kam1 and comparison with its closest relatives.
  • 2019. Allopatric evolution of thermoacidophilic Verrucomicrobia methanotrophs?
  • 2019. Allopatric Evolution among Thermoacidophilic Verrucomicrobial Methanotrophs from Globally Distributed Terrestrial Hot Springs.
  • 2018. Verrucomicrobia Methanotrophs, a Novel Group of Extremophiles Adapted to Acidic Geothermal Environments.
  • 2018. Methane-oxidation in extreme environments; the complete genome sequence of the thermoacidophilic methanotrophic Verrucomicrobia, Methylacidiphilum kamkcatkense.
  • 2018. Methane oxidation by extremophilic verrucomicrobia adapted to acidic geothermal environments.
  • 2018. Methane oxidation by extremophilic Verrucomicrobia adapted to acidic geothermal environments.
  • 2017. Molecular and genomic analysis of thermoacidophilic methanotrophic verrucomicrobia .
  • 2017. Diversity of anaerobic thermophiles in North Sea oil reservoirs and their biotechnological potential.
  • 2017. Diversity and adaptations of extremophilic microorganisms.
  • 2017. Diversity and adaptation of extreme thermophiles.
  • 2016. Extremophilic methane-oxidizing Verrucomicrobia.
  • 2016. Diversity of thermophiles from North Sea oil fields.
  • 2016. Anaerobic thermophiles from deep and hot North Sea oil wells.
  • 2015. Methane oxidation by methanotrophic Verrucomicrobia.
  • 2014. Methane oxidation in extreme environments by thernmoacidophilic methanotrophic Verrucomicrobia .
  • 2014. Diversity and adaptations in Archaea, the third domain of life.
  • 2014. Armenian mines as a source of heavy metal accumulating metalophilic bacilli.
  • 2014. Antarctic cryptic microbial communities: Martian analogs for extremophilic life?
  • 2014. Adaptations to hot and cold: a case study of isocitrate dehydrogenase from extremophiles.
  • 2014. Adaptations to hot and cold: a case study of isocitrate dehydrogenase from extremophiles.
  • 2014. Adaptations to hot and cold: a case study of isocitrate dehydrogenase from extremophiles.
  • 2012. Diversity of Extremophiles.
  • 2012. Chemical strategies of extremophiles.
  • 2011. The GINS complex from Thermoplasma acidophilum may function as a homotetramer in DNA replication.
  • 2011. Life on the edge: Diversity and adaptations of extremophiles.
  • 2011. Extremophiles; adaptation and diversity.
  • 2011. Archaeal DNA replication.
  • 2010. Methane oxidation by a novel lineage of thermo-acidophilic methanotrophic Verrucomicrobia.
  • 2010. High microbial diversity in Antarctic Dry Valley soil microenvironments.
  • 2009. Thermophilic methane oxidizers belonging to the phylum Verrucomicrobia.
  • 2009. The oil reservoir ecosystem: what is happening and who is living down there?
  • 2009. The genome of Thermosipho africanus: lateral genetic connections to Firmicutes and Archaea.
  • 2009. Oil Well ecology: What’s happening and who is living down there?
  • 2009. Molecular basis of the stability of biomoleculees in hot environments.
  • 2009. Methane oxidation in extreme environments by a novel lineage of thermo-acidophilic methanotrophic Verrucomicrobia.
  • 2009. Genetics of thermophiles.
  • 2009. Functional analysis of the DNA replication initiation proteins, Cdc6 and MCM, from Thermoplasma acidophilum.
  • 2009. Extremophiles: basic concepts.
  • 2009. Evolutionary and genetic relatedness of Shigella-like bacteria in aquatic environment of Bangladesh.
  • 2008. Thermophilic biohydrogen production from palm oil mill effluent by mixed culture community with anaerobic sequencing batch reactor (ASBR).
  • 2008. Thermo-acidophilic methane oxidation by Verrucmicrobia extends our perception of biological methane oxidation.
  • 2008. Nitrile hydrolysing enzymes from extremophiles: their role in biocatalysis.
  • 2008. Functional analysis of the MCM and Cdc6 proteins from the thermoacidophilic euryarchaeon Thermoplasma acidophilum.
  • 2007. Synthesis of methyl ester/s by transesterification using lipase produced by a thermophile.
  • 2007. Microbial Diversity of Deep and Hot Petroleum Reservoirs.
  • 2007. Isocitrate dehydrogenase from (hyper)thermophiles; Molecular adaptations to high temperatures.
  • 2006. Microbial diversity in deep petroleum reservoirs.
  • 2006. Isocitrate dehydrogenase from (hyper)thermophiles; Molecular adaptations to high temperatures.
  • 2006. Exploring the microbiology of a deep water coral reef outside mid Norway.
  • 2006. Excision of 1-methyladenine and 3-methylcytosine from DNA by AfAlkA of Archaeoglobus fulgidus.
  • 2005. Sulphate-reducing bacteria and archaea.
  • 2004. The 2.9 Å resolution structure of malate dehydrogenase from the hyperthermophilic archaeon Archaeoglobus fulgidus: mechanisms of oligomerisation and thermal stabilisation.
  • 2004. Natural gas and biotechnology: Bioprospecting of methane-oxidizing bacteria.
  • 2004. Molecular adaptation of isocitrate dehydrogenase to high temperature.
  • 2004. Extremophiles: basic concepts.
  • 2003. X-ray structure analysis of isocitrate dehydrogenase from the hyperthermophile Aeropyrum pernix.
  • 2003. Treasures in the deep biosphere of North Sea oil-wells.
  • 2003. The genomic sequencing of Methylococcus capsulatus (Bath). Repair of alkylated DNA by oxidative demethylation.
  • 2003. Base excision repair of deaminated DNA-cytosine in Archaea: novel genes and mechanisms.
  • 2002. Thermostable proteins from hyperthermophilic microorganisms.
  • 2002. Thermophilic microbes from North Sea oil wells and their thermostable proteins.
  • 2002. Sequencing of the Methylococcus capsulatus genome.
  • 2002. Bakterielle næringskomponenter og metabolisme.
  • 2001. Members of the Thermotogales order isolated from hot North Sea oil field water.
  • 2001. Detection of sulfate reducers indigenous to North Sea oil wells using probes based on dissimilatory sulfite reductase gene sequences.
  • 2000. Thermosipho strain TBA5, a new thermophilic anaerobic bacterium, isolated from a North Sea oil field.
  • 2000. Thermosipho strain TBA5, a new thermophilic anaerobic bacterium, isolated from a North Sea oil field.
  • 2000. Isocitrate dehydrogenase from Aeropyrum pernix.
  • 2000. Isocitrate dehydrogenase from Aeropyrum pernix.
  • 2000. Expression and characterization of a recombinant L-malate dehydrogenase from Archaeoglobus fulgidus strain VC-16.
  • 2000. Expression and characterization of a recombinant L-malate dehydrogenase from Archaeoglobus fulgidus strain VC-16.
  • 2000. Anaerobic microbiology at University of Bergen.
  • 1999. Overview of the research on molecular biology and biochemistry of thermophiles in Bergen.
  • 1996. L-Malate dehydrogenase from the hyperthermophilic archaeon Archaeoglobus fulgidus.
  • 1996. Isolation and characterization of a novel Petrotoga sp. from a North Sea oil-producing well.
  • 1996. Expression of a thermostable isocitrate dehydrogenase from the hyperthermophilic Archaeoglobus fulgidus in Escherichia coli.
  • 1996. Expression of a thermostable isocitrate dehydrogenase from the hyperthermophilic Archaeoglobus fulgidus in Escherichia coli.
  • 1996. Archaeoglobus fulgidus isocitrate dehydrogenase: Purification, characterization, thermostability, genetic analysis and phylogeny.
  • 1996. Archaeoglobus fulgidus isocitrate dehydrogenase: Purification, characterization, thermostability genetic analysis and phylogeny.
  • 1996. A thermosome-encoding gene from the hyperthermophilic archaeon Archaeoglobus fulgidus.
  • 1996. A thermosome-encoding gene from the hyperthermophilic archaeon Archaeoglobus fulgidus.
  • 1996. A chaperonin-encoding gene from the hyperthermophilic archaeon Archaeoglobus fulgidus.
  • 1996. A chaperonin-encoding gene from the hyperthermophilic archaeon Archaeoglobus fulgidus.
Editorial
  • 2019. Thermophilic and Halophilic Extremophiles in Eurasian Environments. Frontiers in Microbiology.
Academic anthology/Conference proceedings
  • 2018. Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications. Springer.
Doctoral dissertation
  • 2016. Fish protein hydrolysates based on Atlantic salmon by-products -Enzyme-cost efficiency and characterization of sensory, surface-active and nutritional properties. Dissertation for the degree of phisosophiae doctor (PhD).
  • 2014. Structure-function analysis of isocitrate dehydrogenase subfamily II: Adaptations to hot and cold.
  • 2009. Discovery of novel methanotrophs from extreme environments.
  • 2008. Regulation of DNA replication in Archaea; Functional analysis of DNA replication initiation proteins in Thermoplasma acidophilum.
  • 2007. Microbiological analyses of produced water from a high-temperature oil formation in the North-Sea.
  • 2006. Isocitrate dehydrogenase from extremophiles; Molecular adaptations to high temperatures.
  • 2006. DNA Base Excision Repair of the Hyperthermophilic Archaeon Archaeoglobus fulgidus.
Academic chapter/article/Conference paper
  • 2018. Microbial Diversity of Terrestrial Geothermal Springs in Lesser Caucasus. 37 pages.
  • 2018. Geobacillus and Anoxybacillus spp.from Terrestrial Geothermal Springs Worldwide: Diversity and Biotechnological Applications. 48 pages.
  • 2013. Biocatalysis through thermostable lipases: adding flavor to chemistry. 23 pages.
  • 2005. Sulphate-reducing Bacteria and Archaea. 20 pages.
  • 2004. The Microbial Diversity of Deep Subsurface Oil Reservoirs. 19 pages.
  • 2001. Isocitrate Dehydrogenase, Malate Dehydrogenase, and Glutamate Dehydrogenase from Archaeoglobus fulgidus. Hyperthermophilic Enzymes, Part B. Michael W.W.Adams and Robert M. Kelly (eds.). 14 pages.
Abstract
  • 2015. Heavy metal resistance of Bacillus subtilis AG4 isolated from the Sotk Gold Mine in Armenia. The FEBS Journal.
  • 2013. The effect of combined heavy metal ions on copA, nikA and czcD genes expression of metal-resistant bacilli. The FEBS Journal. 231-231.
Errata
  • 2014. Erratum: Using network analysis to explore co-occurrencepatterns in soil microbial communitie (ISME Journal (2014) 8 (952) DOI:10.1038/ismej.2013.236). The ISME Journal. 952.
  • 2014. Erratum: Evidence for successional development in antarctic hypolithic bacterial communities (ISME Journal (2014) 8 (952) DOI:10.1038/ismej.2013.230). The ISME Journal. 952.
Academic literature review
  • 2009. Environmental, genomic and taxonomic perspectives on methanotrophic Verrucomicrobia. 293-306.

More information in national current research information system (CRIStin)

CV

Education

Cand.real. (M.Sc.) in microbiology 1981, University of Bergen, Norway.

Dr.philos. 1992, University of Oslo, Norway (bacteriophage molecular biology).

 

Career/Employment

· Dept. of Physical Chemistry, Norwegian Institute of Technology, Trondheim, Norway: Research assistant, 1981-1982.  Membrane transport in halobacteria.

. Dept. of Medical Biology, University of Tromsø, Tromsø, Norway: Research assistant, 1982-1985. Gene regulation in the Escherichia coli P2:P4 system.

. Dept. of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, USA: Research scientist, 1986-1987.  Regulation of gene expression in the P2:P4  bacteriophage system.

·  Laboratory of Microbial Gene Technology, The Norwegian Research Council/The Norwegian   Agricultural University, Ås, Norway: Research scientist, 1988-1993.  Molecular biology and  biotechnology of lactic acid bacteria and phages.

·  Dept. of Biology (previous Dept. of Microbiology), University of Bergen, Bergen, Norway: Associate professor, 1993-1996; full Professor in microbiology from 1996 - present. From 2007 also affiliated with the Centre for Geobiology (a CoE) at Univ. of Bergen. Molecular biology and biochemistry of extremophiles; anaerobic microbiology; methane-oxidising bacteria; environmental microbiology.

 

International experience

·  Research scientist at Dept. of Microbiology and Immunology, Virginia Commonwealth University, Richmond, Virginia, USA 1986-1987. Gene regulation.

·  Visiting Professor at the Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland, USA, 1998-1999 (one year). Molecular biology of hyperthermophiles.

· Coordinator for the NorFA-network Biology of Thermophiles, 1998 – 2003.

· Coordinator of the INTAS program Reduction of toxic metals and radionuclides by thermophilic prokaryotes. 2002 – 2005.

·  Coordinator of the NUFU program Assessment of microbial pollution, diversity and community structure in freshwater resources in Bangladesh using molecular techniques, 2003 – 2012.

· Convenor for the 9th International Thermophiles conference in Bergen in September 2007.

 

Some of my active collaborators

· Prof. Arne Smalås, the Norwegian Structural Biology Center (NORSTRUCT), University of Tromsø, Norway (Protein structure)

· Prof. Svein Bjelland, University of Stavanger, Norway (DNA repair in hyperthermophiles)

· Dr. Elizaveta Bonch-Osmolovskaya, Institute of Microbiology, the Russian Academy of Sciences, Moscow (Thermophilic bacteria from hot springs)

· Dr. Rudolf Ladenstein, Center for Structural Biochemistry, Karolinska Institutet, NOVUM, Stockholm, Sweden (Protein structure and stability)

· Dr. Zvi Kelman, Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute (Regulation of archaeal cell-cycle)

· Dr. Poonsuk Prasertsan, Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Thailand (Biohydrogen)

· Professor Don Cowan, University of the Western Cape, South Africa (Antarctic metagenomics)

· Professor Sirajul Islam Khan, Department of Microbiology, University of Dhaka, Bangladesh (enteropathogens in tropical freshwaters)

 

Patent applications

· Lillehaug D and Birkeland NK (1994): Bacteriophage ΦLC-3-based vector system for transformation of bacteria. WO 9419460.

· Jonassen I et al. (2002): Systems for expression of heterologous proteins in Methylococcus capsulatus. WO 02055549.

· Eisen JA et al. (2002): Metylococcus capsulatus genes and DNA array for the determination of gene expression in Metylococcus capsulatus. WO 02055655. 

· Birkeland NK and Islam T: Novel methanotroph (biological conversion of methane to methanol). Pending.

 

Current and previous projects, networks and funding for advanced equipment:

2018 - 2021: (Eurasian - Norwegian) network for research-based higher education in microbial biotechnology. Diku.

2017 - 2021: Methane oxidation by extremophilic Verrucomicrobia adapted to geothermal
environments. Research Council of Norway. FRIMEDBIO.

2017 - 2020: Thermostable isomerase processes for biotechnology (TIPs). ERA-IB/BIOTEK2021.

2017 - 2019: (Eurasian - Norwegian) Network for improving research based higher education in basic and applied microbiology.

2015 - 2016: Extremophilic Archaea in Bulgarian geothermal environments. BG09 Programme. EEA Scholarships Fund.

2013 - 2016: THERMOGENE - Novel thermostable enzymes for industrial biotechnology. NFR/ERA-NET Industrial Biotechnology project

2014 - 2015: Integrative analysis of extremophiles in search for new biotechnological solutions. NILS Science and Sustainability (ES07). EEA Grant.

2013 - 2015: Eurasian-Norwegian network for higher education and research in environmental microbiology and biotechnology

2011 - 2015: Cooperation in environmental microbiology between Armenia and Norway. SIU

 2012: Geomicrobiology for bioenergy. The Agricultural Research Development Agency, Thailand.

2011 - 2014: Cooperation with Armenia in microbiology of extreme environments. SIU/UD. 1.8 mill NOK.

2011-2014: Biological methane oxidation by methanotrophic Verrucomicrobia under  hot and acidic conditions; evolution of an ancient metabolic trait. NFR, 3.5 mill NOK.

 2009-2012: Subsurface metagenomics, functional microbial diversity analysis and gene discovery in deep and hot petroleum reservoirs. VISTA. 2.5 mill NOK.

2010: Is methane oxidation an ancient metabolic trait? Centre for Geobiology, UiB. 180.000 NOK.

2007-2010: Metagenomics and gene discovery in Antarctic terrestrial habitats. The Research Council of Norway/The South-Africa programme. 898.000 NOK.

2009-2012: The Nordic Archaea Network. NordForsk.

2008: Comparative genomics of Archaeoglobus fulgidus strain 7324, a subsurface hyperthermophilic sulfate-reducing archaeon isolated from a hot North-Sea oil-well. University of Bergen/ Friforsk. 300.000 NOK.

2003-2011: Assessment of microbial pollution and diversity of Escherichia coli and Shigella in freshwater resources in Bangladesh. NUFU. 6.5 mill NOK.

2007-2010: Silicon Cell Model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation. SysMo.

2004-2007: Metanspisende bakterier i havbunnsmiljø. VISTA. 1.5 millNOK.

2006-2007: Archaeoglobus fulgidus strain 7324: a unique archaeal sulfate reducer. The Research Council of Norway/ DAAD. 50.000 NOK.

2003-2007: Natural gas and biotechnology (NABI): bioprospecting of methane-oxidizing bacteria. The Norwegian Research Council/ Bioprospecting programme. 6.7 mill NOK.

2003-2007: Biological adaptations to extreme temperatures. The Norwegian Research Council/ Strategic university programme. 5.9 mill NOK.

2003-2007: Archaeal cell-cycle regulation. The Research Council of Norway. 1.7 mill NOK.

2006: Robot for automated picking of bacterial colonies (GenePix2xt). The Research Council of Norway. 1.7 mill NOK.

2002-2006: Biotechnology of intraterrestrial microorganisms. The Research Council of Norway/ Bioprospecting. 1.6 mill NOK.

2003-2005: Reduction of toxic metals and radionuclides by thermophilic prokaryotes. INTAS. 450.000 NOK.

2002-2004: Reduction of metals by thermophilic prokaryotes. NATO. 100.000 NOK.

1999-2003: Biology of Thermophiles. Nordic network. NorFA. 1.5 mill NOK.

1997-2001: VIRTUE: Bioaktive substanser. Wallenbergfondet. 475.000 NOK.

1997-1999: Termoresistens hos Archaeoglobus. The Research Council of Norway. 288.000 NOK.

1996-1999: Dissimilatorisk sulfitt reduktase fra oljebakterier. The Research Council of Norway. 1.2 mill NOK.

1995: Iso-sitronsyre dehydrogenase i Desulfobacter. The Research Council of Norway. 412.000 NOK.

1994: Generell drifts- og utstyrsbevilgning. The Research Council of Norway. 150.000 NOK.