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  • E-mailRunar.Stokke@uib.no
  • Phone+47 55 58 38 33+47 908 36 074
  • Visitor Address
    Thormøhlens gate 53 A/B
  • Postal Address
    Postboks 7803
    5020 Bergen

My research has a strong focus on deep-sea hydrothermal vent ecosystems.

In spite of the recent technological and analytical advancement in the field of hydrothermal vent research and metagenomics, fundamental and systematic knowledge on the biodiversity of microbial life and their functional role in the unique deep sea vent environments can still be considered as in its infancy and in the exploratory phase. This hampers our ability to evaluate the global ecological role of the hydrothermal vent biota. Since genomic knowledge is still missing for most of the uncultivated lineages in hydrothermal vents, there is a comprehensive knowledge need and potential to discover new microbial lineages with novel and primordial metabolisms, as well as new metabolic inter-dependencies and species interactions. At present, knowledge is also lacking on the encoded capacity of hydrothermal vent microorganisms to produce bioactive compounds to be used in communications between and across trophic levels in the ecosystem, and in turn represent compounds for human benefit.

Integrated studies combining genome-centric metagenomics with metatrascriptomics and high-resolution microscopy is central in my research to infer potential functions of uncultured novel hydrothermal vent microbial lineages, and identifying microbial metabolic interdependencies and co-operations.

 

 

  • Show author(s) 2021. Iron Oxidizers and Sheath-forming Zetaproteobacteria in Biofilms of the Newly Discovered Fåvne Hydrothermal Vent Field.
  • Show author(s) 2021. Exploring codon adjustment strategies towards Escherichia coli-based production of viral proteins encoded by HTH1, a novel prophage of the marine bacterium Hypnocyclicus thermotrophus. Viruses.
  • Show author(s) 2021. Alginate Degradation: Insights Obtained through Characterization of a Thermophilic Exolytic Alginate Lyase. Applied and Environmental Microbiology. 16 pages.
  • Show author(s) 2021. Alginate Degradation: Insights Obtained through Characterization of a Thermophilic Exolytic Alginate Lyase. Applied and Environmental Microbiology.
  • Show author(s) 2020. “Candidatus Ethanoperedens,” a thermophilic genus of archaea mediating the anaerobic oxidation of ethane. mBio. 1-18.
  • Show author(s) 2020. Tailoring hydrothermal vent biodiversity towards improved biodiscovery using a novel in-situ enrichment strategy. Frontiers in Microbiology.
  • Show author(s) 2020. Enzyme discovery from the edge of (sequence) space.
  • Show author(s) 2020. Bringing deep-sea enzymes to light.
  • Show author(s) 2019. Identification of key-players in anaerobic alkane oxidation in the diffuse venting Barite Field at the Loki´s castle.
  • Show author(s) 2019. Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. PLOS ONE.

More information in national current research information system (CRIStin)

Current and previous projects

 

PRINCIPAL INVESTIGATOR:

2021-2024  DeepSeaQuence; Uncovering the metabolic secrets and capacity of Arctic deep-sea hydrothermal vent microbiomes. Research Council of Norway, FRINATEK Researcher Project

 

PROJECT COLLABORATOR:

2021 - UiB Centre for Deep-Sea Research - Trond Mohn Foundation

2021 - 2024 Eco-Safe Ridge Mining: environmental risk studies towards sustainable deep-sea mining on the Mid-Ocean Ridge in Norway

2021 - 2023 RAS-TOOLS: A microbial toolbox for RAS production and innovation

2017-2021: KG Jebsen centre for Deep-Sea Research (leading WP6; Biodiscovery and Bioprospecting)

 

 

Key qualifications:

  • Advanced Bioinformatics
  • Proteomics and Mass spectrometry
  • Metagenomics, metaproteomics and metatranscriptomics
  • Genome assembly and annotation
  • Cultivations of extremophilic microorganisms
  • Protein expression and purification
  • Protein chemistry and structural biology
  • Protein stability

Current PhD and master students  (main or co- supervisor)

Petra Hribovsek (UiB) - Investigating the ecology, evolution and metabolism of novel deep-sea hydrothermal lineages.

Francesca Vulcano (UiB) - Short chain hydrocarbon degraders in hydrothermal vents systems on the Arctic Mid-Ocean Ridge.

Victora Røyseth (UiB) - Isolation and enzyme characterization of proteolytic hydrothermal vent microorganisms

Emily Maria Denny (UiB) - Shaping of hydrothermal microbiomes in response to energy availability

 

Graduated

PhD Hasan Arsin - Sequence-based enzyme discovery from marine microbial biodiversity: Multiple approaches for the heterologous expression of genes in Escherichia coli - 2021

PhD Anders Schouw - Isolation and genome analysis of Vallitalea guaymasensis L81; energy metabolism, syntrophy and applications - 2018

PhD Juliane Wissuwa - The Arctic mid-Ocean Ridge Vent Fields - A valuable Resource for Marine Bioprospecting? - 2016

PhD William P. Hocking - Energy conservation mechnisms in the sulfate respiring  archaeon Archaeoglobus fulgidus VC16-assessed by transcriptome analyses and comparative genomics. - 2015

Msc. Sondre Olai Spjeld - Metagenomics-based discovery of novel carbohydrate degrading enzymes from deep-sea hydrothermal in situ enrichments; expression, purification and characterization - 2021

MSc. Sepideh Mostafavi - Metagenome-based analysis of the functional potential of a marine sediment community dominated by ANME-2c. - 2015

MSc. Sophie Radeke - Diversity, function and interactions of anaerobic methanotrophs in Loki ́s Castle Vent Field. - 2015

MSc Carina Magnussen - Genekspresjon av karbonmonoksid dehydrogenase/acethyl-CoA syntase komplekset og sat-ORF2-aprBA operonet i Arhcaeoglobus fulgidus. - 2010

 

Research groups

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