Response to titanium in an oral implant model

Histologic and immunohistochemic evaluation of non-perforated oral mucosa covering submerged titanium implants

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Recent reports have questioned if metal sensitivity may arise from exposure to titanium. The objective of the first study was to histologically evaluate non-perforated mucosa covering submerged maxillary titanium implants with regard to induced tissue reactions. Twenty patients without previous implants were included. After initial examination, the bone crest areas destined for dental implant placement were exposed, and threaded external hex dental implants were inserted. Prior to wound closure, a full mucosal tissue slice was biopsied from the edge of the mucoperiosteal flap (baseline). At the abutment connection, biopsies were taken by a 6-mm punch. Tissue reactions were analyzed by coded histometric analysis at four defined areas at increasing distance from the oral epithelium, including ratios of inflammatory cells (IC)/epithelial cells, IC/fibroblasts, and number of dense particles. Paper in J Periodontol 2006 - PubMed
These sections will be examined for the presence of T cells (CD3+) and subgroups of T cells (CD4+ / CD8+), B cells (CD 19), macrophages (CD68) and Langerhans cells (CD1a+), using routine immunohistochemical methods.
Several dense particles were observed in the H&E sections. Futher studies will focus on how to record Ti particles, also in a nanoparticle range, using micro-analytical tecniques.

PhD student: R. Flatebø.

Supervisors: K. N. Leknes; N. R. Gjerdet; A. Chr. Johannessen;  P. J. Høl (co-supervisor)