Workshop on Single Molecule-based Super-resolution Microscopy: from Acquisition to Analysis, from Theory to Applications
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Are you studying proteins or RNAs in the plasma membrane, cytoplasm, nucleus, endoplasmic reticulum, or mitochondria ? Are you curious whether these molecules move freely or undergo confined movement, how fast and how far far they move, how they are dynamically arranged at nanoscale resolution in live cells, or how they change behavior after a pharmacological stimulation or in a disease model ? If so, then you should not miss this workshop at the Department of Biomedicine at UiB.
Scope of teaching and/or hands on sessions:
1) Single particle tracking of endogenous proteins in the plasma membrane using Quantum Dots (Lecture + Hands-on session)
2) Single particle tracking of proteins in the nucleus by genetic fusion of Halo tag to the target protein and synthetic fluorescent ligands (Lecture + Hands-on session)
3) Single particle tracking of proteins by genetic fusion of a photoswitchable/photoactivatable protein to the target protein (Lecture + Hands-on session)
4) Single particle tracking of RNA in live cells (Lecture)
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The Program
The core facility for Single-Molecule Localization Microscopy (SMLM) located at the Department of Biomedicine (UiB) has recently been opened to all users. SMLM is a state-of-the-art technology, which dramatically improves spatial resolution over standard, diffraction-limited microscopy. Major biological insights have been made using this technology into the spatial and temporal organization of proteins and nucleic acids within subcellular compartments (such as nucleus, cytoplasm and plasma membrane) at nanoscale resolution. To help potential users become familiar with this cutting-edge technology, we will organize at least two workshops (1st in 2022, 2nd in 2023) addressing both theoretical and experimental considerations for SMLM, including fluorescent labeling, sample preparation, hardware requirements, image acquisition, analysis in live cells. Several outstanding scientists, who have developed novel labeling/acquisition/analysis for single molecule-based super-resolution imaging of protein or RNA, have confirmed their participation. This is a great opportunity to have a face-to-face interaction with these scientists, and bring your research questions and get inputs from them.