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StemBoneReg-Trond Mohn Stiftelse

The group has received funding from the Trond Mohn Foundation for the project "Reconstruction of mandibular bone using stem cells and biomedical engineering approaches" (2018 - 2022).

KM
Professor Kamal Mustafa and project leader Cecilie Gjerde
Photo:
BFS

Increased emphasis is being placed on translational research discoveries, which will bring the use of stem cells in laboratory studies into the clinical setting. However, only few early stage clinical studies with a limited number of patients have proven safety of this novel regenerative treatment. According to the clinical database (www.clinicaltrials.gov) and Medline, it appears that our research is at the forefront of regenerative medicine as we have successfully performed an early stage pilot clinical study using stem cell therapy in 11 patients for reconstruction of alveolar bone with excellent results at the 24 month examination. These results would pave the way for improved and well-designed phase II clinical trial using stem cell therapy for bone regeneration.

Therefore, 20 new patients will be recruited and treated is a phase II clinical trial aiming for regeneration of severely resorbed mandibular bone to dental implants. The primary objective of this clinical study will be to assess the safety and effectiveness of the stem cell therapy and biomaterials to regenerate mandibular bone in width and height in order to enable adequate implant placement. This new interventional therapy will be compared retrospectively if the use of bone marrow mesenchymal stem cells (MSC) and biomaterial as bone grafting engineered construct is equally good or better than the gold standard of bone augmentation, i.e. the autologous bone graft from mandibular ramus. The clinical protocol which has been accepted by the Ethics Authority (REK, 2013/1284) and Norwegian National Medicine Agency (NOMA, 13/12062) will be modified and re-submitted to REK and NOMA after the necessary amendments.

Bone augmentation and regeneration will be achieved by implantation of a synthetic bone substitute and culture expanded autologous MSC covered by a synthetic membrane. Innovative medical imaging and histology of core biopsies before dental implants will ensure the evaluation of bone regeneration in patients. The safety and efficiency of the treatment will be assessed by evaluating the occurrence of adverse effects, monitoring cells after transplantation, assessing acceptability of this approach and its impact on the patient’s quality of life and other patient reported outcomes. The data and knowledge generated from the project will be utilized to develop translational approaches for regenerative therapies of different types of bone defects and support the establishment of the new Ex Vivo Laboratory planned to be opened in Bergen at Haukeland University Hospital in 2020. Additionally, the project will also include development of novel biomaterials for bone regeneration.

 

Project presentation in national or international conferences

  • 2019, Oct. Tissue Engineering & Regenerative Medicine Internatinal Society (TERMIS) AP Chapter and the 7th Asian Biomaterials Congress, Brisbane, Australia. Oral presentation: Mohammed Yassin. Title: 3D printed copolymer scaffolds for rat segmental femoral defect.
  • 2019, Oct. Tissue Engineering & Regenerative Medicine Internatinal Society (TERMIS) AP Chapter and the 7th Asian Biomaterials Congress, Brisbane, Australia. Oral presentation: Cecilie Gjerde. Title: Stem Cells in Bone Regeneration, A Clinical Trial with Three Year Follow Up.
  • 2019, Oct. Tissue Engineering & Regenerative Medicine Internatinal Society (TERMIS) AP Chapter and the 7th Asian Biomaterials Congress, Brisbane, Australia. Poster presentation: Kamal Mustafa. Title: Wood-Based Nanocellulose for Bone Tissue Engineering.
  • 2019, Oct. Tissue Engineering & Regenerative Medicine Internatinal Society (TERMIS) AP Chapter and the 7th Asian Biomaterials Congress, Brisbane, Australia. Poster presentation: Salwa Suliman. Title: Influence of humanised isolation and culture conditions on bone marrow derived mesenchymal stromal cells.
  • 2019, Oct. Biofabrication 2019, Columbus OH, USA, Oral Presentation: Jannika Paulamäki. Title: Development of Nanostructured Alginate-Gelatin -Based Inks for 3D Bioprinting of Bone Cells.
  • 2019, Oct. Foredrag for rektoratet ved UiB om stamcelleforskningen. Invited Speaker: Cecilie Gjerde.
  • 2019, Oct. Bergen Stem Cell Consortium meeting, Bergen Norway. Invited Speaker: Cecilie Gjerde. Title: stamcelleforskningen.
  • 2019, Oct. Bergen Stem Cell Consortium meeting, Bergen Norway. Oral Presentation: Shuntaro Yamada. Title: Plasma activation of polymeric biomaterial enhanced initial adhesion and seeding efficiency of mesenchymal stromal cells.
  • 2019, Sept. European Association for Osseointegration (EAO) Meeting, Lisbon, Portugal. Keynote Speaker: Kamal Mustafa, Plenary session. Title: Are stem cells the implants of the future.
  • 2019, Sept. Orthopedic department, Int conf Merch, Norway. Invited Speaker: Cecilie Gjerde. Title: Cell therapy induced regeneration in severely atrophied mandibular bone.
  • 2019, Sept. Bergen Dental Health Center, Autumn meeting, Bergen, Norway. Invited Speaker: Cecilie Gjerde. Title: Beinoppbygging med egne stamceller Bergensmodellen.
  • 2019, Sept. NYSCO Annual Meeting, Oslo, Norway. Invited Speaker: Kamal Mustafa. Title: From bench to chairside: Stem cells in bone augmentation.
  • 2019, Sept. NYSCO Annual Meeting, Oslo, Norway. Poster presentation: Jannika Paulamäki. Title: Biomimetic nanocellulose-nanohydroxyapatite scaffolds for bone tissue engineering.
  • 2019, Sept. NYSCO Annual Meeting, Oslo, Norway. Poster presentation: Shuntaro Yamada. Title: In-silico modelling as an expedient tool for the assessment of bioreactor-induced dynamic conditions.
  • 2019, June. Scandinavian Society for Biomaterials 2019 Conference, Helsinki, Finland. Oral presentation: Jannika Paulamäki. Title: Characterization of the Printability of Alginate-Gelatin Bioink Blended with Nanocellulose.
  • 2019, June.  IADR, Vancouver, Canada. Oral presentation: Cecilie Gjerde. Title: Stem cells for bone regeneration, the future is now!
  • 2019, June. Stem cell research for x-ray, HUS, Norway. Invited Speaker: Cecilie Gjerde. Title: Beinoppbygging med egne stamceller Bergensmodellen
  • 2019, May. Revisorene ved UiB, Forelesning om forskningen Norway. Invited Speaker: Cecilie Gjerde.
  • 2019, May. BioVaria Meeting, Munich, Germany. Invited Speaker: Kamal Mustafa. Title: Bone from the 3D printer.
  • 2019, Feb. Research meeting at University of Tromsø, Tromsø, Norway. Invited Speaker: Cecilie Gjerde. Title: Tissueengineering in Bergen.
  • 2019, Feb. AMCHA 2019 Meeting, Sri Lanka. Invited Speaker: Kamal Mustafa. Title: Stem cells in bone regeneration, a systematic approach and pilot study in humans.
  • 2019, Jan. TekPRAT, Bihuben, HUS. Invited Speaker: Cecilie Gjerde. Title: 3D printing event arr av HelseVest, Bg Næringsråd mv Stamceller i beinregerasjon.
  • 2019, Jan. Forskningsmøte Universitetet i Tromøs. Invited Speaker: Cecilie Gjerde. Kurs: forskning for 2. års studentene
  • 2019, Jan. Studentseminar, Geilo, Norway. Invited Speaker: Cecilie Gjerde. Title: Stamceller i beinregerasjon.
  • 2018, June. Joint Workshop in Stem Cells and Regenerative Medicine, Tokyo, Japan. Invited Speaker: Kamal Mustafa. Title: MSC and Biomaterial for Bone Regeneration.
  • 2018, June.  Europerio, Amsterdam. Invited Speaker: Cecilie Gjerde. Title: stamcelleforskningen.
  • 2018, Sep. Tekna. Invited Speaker: Cecilie Gjerde Title: stamcelleforskning.
  • 2018, Nov. Ansa, Warszawa Invited Speaker: Cecilie Gjerde. Title:Stem cells in bone regeneration.           
  • 2018, Des. Åpen post NRK, Invited Speaker: Cecilie Gjerde, Presenterer forskningen.

Peer reviewed publications

-        Hassan MN, Yassin MA, Suliman S, Lie SA, Gjengedal H, Mustafa K. The Bone Regeneration Capacity of 3D-Printed Templates in Calvarial Defect Models: A Systematic Review and Meta-Analysis. Acta Biomaterialia 91:1-23. doi: 10.1016/j.actbio.2019.04.017 (2019). https://www.ncbi.nlm.nih.gov/pubmed/30980937

-        Ojansivu M, Rashad A, Ahlinder AE, Massera J, Mishra A, Syverud K, Finne-Wistrand A, Miet-tinen S, Mustafa K. Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells. Biofabrication. doi: 10.1088/1758-5090/ab0692 (2019). https://www.ncbi.nlm.nih.gov/pubmed/30754034

-         Rashad A, Suliman S, Mustafa M, Pedersen TO, Campodoni E, Sandri M, Syverud K, Mustafa K. Inflammatory Responses and Host Tissue Reactions to Wood-Based Nanocellulose Scaf-folds. Materials Science & Engineering C Mater Biol Appl. 97:208-221 (2019). https://www.sciencedirect.com/science/article/pii/S0928493118324135?via%...

-         Gjerde C, Shanbhag S, Neppelberg E, Mustafa K Gjengedal H. Patient experience following iliac crest-derived alveolar bone grafting and implant placement. Submitted manuscript.

Funding and Awards

  • 2019, Oct. Bergen Stem Cell Consortium meeting, Bergen Norway. Oral Presentation by Shuntaro Yamada. Title: Plasma activation of polymeric biomaterial enhanced initial adhesion and seeding efficiency of mesenchymal stromal cells. (Winner of Lightning talk competition)
  • 2019, Oct. Biofabrication 2019, Columbus OH, USA, Oral Presentation: Jannika Paulamäki. Title: Development of Nanostructured Alginate-Gelatin -Based Inks for 3D Bioprinting of Bone Cells. (ISBF Travel award)
  • 2019, June. Scandinavian Society for Biomaterials 2019 Conference, Helsinki, Finland. Oral presentation: Jannika Paulamäki. Title: Characterization of the Printability of Alginate-Gelatin Bioink Blended with Nanocellulose. (Rapid-Fire Poster Competition, 1st prize)
  • 2019, Oct. New funding of 12,3 mill.kr for project “Study of Mesenchymal Autologous stem cells as Regenerative Treatment for Multiple Sclerosis (SMART-MS)”.  http://kliniskforskning.rhf-forsk.org/149-millioner-kroner-til-klinisk-b... (use of MSC for treatment of MS)
  • 2018, Dec. FORNY-midler til 3D-printer deler av ditt skjelett. https://www.visinnovasjon.no/2018/12/forny-midler-tildelt-tre-uib-prosje...

Book chapter

Stamceller. Kroppens superheltar Av: Øystein Runde, Utgiver: Gyldendal Norsk Forlag, Nettsted: https://www.gyldendal.no/Barneboeker/E-boeker/Stamceller-E-bok, 2017, ISBN: 9788205498372

 

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