NanoSafety & NanoMedicine Platform
Leader: Mihaela Roxana Cimpan, Professor, Biomaterialer – Department of Clinical Dentistry
Microfluidic platform for real-life exposure to nanomaterials
Zetasizer Nano ZSP (Malvern, UK)
Characterization of proteins and nanoparticles, size and zeta potential measurements. It includes a "Protein Measurement" option for protein mobility measurements.
The system incorporates a two-angle particle and molecular size analyzer for the enhanced detection of aggregates and measurement of small or dilute samples, and samples at very low or high concentrations using dynamic light scattering with ‘NIBS’ optics. The ZSP also incorporates a zeta potential analyzer that uses electrophoretic light scattering for particles, molecules and surfaces, and a molecular weight analyzer using static light scattering.
Light Source He-Ne laser 633nm, Max 10mW
Particle and molecule size, Translational diffusion, Electrophoretic mobility, Zeta potential of particles at high and low concentrations, Dynamic and Electrophoretic Light Scattering, Viscosity and viscoelasticity of protein and polymer solutions, Concentration, MW, A2, kD.
- Zeta potential measurement of proteins and nanoparticles
- Size measurement from 0.3nm (diameter) to 10 microns using Non-Invasive Back Scatter technology.
- Zeta potential of surfaces using accessory cell.
- Molecular weight measurement down to 980Da.
- Microrheology option to measure viscosity and viscoelasticity.
- Protein size measurement sensitivity, 0.1mg/mL
- Sample concentrations from 0.1ppm to 40%w/v.
- Built-in protein calculators, including protein charge, A2, kD, and molecular conformation.
- Automation of measurements using an autotitrator option.
- Chromatography detector capability to enable use as a size detector with GPC / SEC or FFF.
- Optical filter option to improve measurements with fluorescent samples.
Sonicator VCX130, Vibra-Cell, 130W (Sonics & Materials)
Suitable for sonication of agglomerated and aggregated nanoparticles. Includes an energy meter, variable power output control, a 1 second to 10 hour timer plus a programmable pulser to safely process a wide range of organic and inorganic materials from 150 microlitres to 150 millilitres. The standard 6mm probe is suitable for processing volumes from 10 to 50ml. To process volumes smaller than 10ml or larger than 50ml a different probe would be required.
Label-free: Impedance based biosensor technology does not require markers or dyes. Interference-free from Nanomaterials and Nanoparticles perturbation.
Real-time kinetic readouts: Obtains data continuously from seconds to days. Simultaneously monitor up to three plates, without scheduling conflicts. Easy workflow: Plate cells, expose to Nanomaterials and begin monitoring.
Measures cell number, size, morphology, and attachment properties, with the ability to perform kinetic analysis of cell invasion/migration (CIM).
Ampha Z30: Impedance-based, Label free Flow Cytometer (Amphasys AG, Switzerland)
AmphaZ30 (Amphasys AG, Lucerne, Switzerland) is an impedance flow cytometer for high-throughput single-cell characterization without optical components. Single cells pass through a micrometer-sized channel in a chip equipped with microelectrodes. The electrical impedance changes when a cell passes through the applied alternating current (AC) field, permitting cell detection and impedance measurement. The measured impedance is used to assess cellular size, membrane capacitance, and cytoplasm resistance and to differentiate between live and cell cells and modes of cell death.
Cyclic voltammetry to study nanomaterial-mediated oxidative stress
We use cyclic voltammetry (CV) to study if nanomaterials may cause oxidative stress in biological systems. The total antioxidant capacity (TAC) of a biofluid reflects the amount of antioxidants available to counteract oxidative stress.
In-house printing of disposable electrodes for cyclic voltammetry
We make use of a PCB printer (Voltera One) to dispense conductive inks on PET films for fabrication of cost-effective and disposable electrodes.