Hjem
Klinisk institutt 2

Measles Virus Infected Cells, RNA Editing and Macroarray Hybridization

Hovedinnhold

Among previous works, the most significant results were the discovery of RNA editing based upon cDNA-cloning and sequencing of measles virus grown in Vero cells (Kalland et al., 1989) and the use of differential hybridization of labelled cDNA probes obtained from either infected or uninfected Vero cells to clone measles virus sequences (Kalland et al., 1991). In this manuscript we proposed: " These experiments infer that when the amount of target DNA is carefully controlled, cDNA probes are not only useful for the identification of differentially expressed cloned transcripts, but also to monitor hundreds of defined transcripts with one labelled cDNA probe in one single hybridization experiment. We propose the preparation of filters imprinted with exact amounts of target sequences for this purpose." This proposal from the 1980s was also the underlying concept when we embarked on our current global gene expression analysis project in the year 2000. By then the potential of the macroarray and microarray principle was vastly increased due to sequencing of the entire human genome, new robotic printing technology and powerful bioinformatic algorithms.