Hjem
  • E-postarne.raasakka@uib.no
  • Besøksadresse
    Stanford University School of Medicine
    1201 Welch Rd.
    94305 Stanford
  • Postadresse
    Postboks 7804
    5020 Bergen

Jeg arbeider som postdoktor i Petri Kursula laboratorium på UiB og som gjesteforsker i Zuchero laboratorium på Stanford Medicine. Klikk her for en bedre beskrivelse av forskningsprosjektet mitt på engelsk.

h-index: 10 (Web of Science)

Publikasjoner: 31 (siste 5 kalenderår: 19)

 

2022

  • Krokengen O.C., Raasakka A. & Kursula P. (2022) The intrinsically disordered protein glue of myelin: Linking AlphaFold2 predictions to experimental data. bioRxiv 2022.09.13.507838. doi: 10.1101/2022.09.13.507838. Preprint.

  • Ruskamo S., Raasakka A., Pedersen J.S., Martel A., Škubník K., Darwish T., Porcar L. & Kursula P. (2022) Human myelin proteolipid protein structure and lipid bilayer stacking. Cell Mol Life Sci. 79(8): 419. doi: 10.1007/s00018-022-04428-6.

  • Markusson S.*, Hallin E.I.*, Bustad H.J., Raasakka A., Xu J., Muruganandam G., Loris R., Martinez A., Bramham C. & Kursula P. (2022) High-affinity nanobodies as tools for structural and functional studies on mammalian Arc. PLoS ONE 17(6): e0269281. doi: 10.1371/journal.pone.0269281.

  • Sutinen A.*, Nguyen G.T.T*, Raasakka A., Muruganandam G., Loris R., Ylikallio E., Tyynismaa H., Bartesaghi L., Ruskamo S. & Kursula P. (2022) Structural insights into Charcot-Marie-Tooth disease-linked mutations in human GDAP1. FEBS Open Bio. 12(7): 1306-1324. doi: 10.1002/2211-5463.13422.

  • Kind L., Raasakka A., Molnes J., Aukrust I., Bjørkhaug L., Njølstad P.R., Kursula P. & Arnesen T. (2022) Structural and biophysical characterization of transcription factor HNF-1A as a tool to study MODY3 diabetes variants. J Biol Chem. 298(4): 101803. doi: 10.1016/j.jbc.2022.101803.

2021

  • Mahootchi E.*, Raasakka A.*, Luan W., Muruganandam G., Loris R., Haavik J. & Kursula P. (2021) Structure and substrate specificity determinants of the taurine biosynthetic enzyme cysteine sulphinic acid decarboxylase. J Struct Biol. 213(1): 107674. doi: 10.1016/j.jsb.2020.107674.

  • Nguyen G.T.T.*, Sutinen A.*, Raasakka A., Muruganandam G., Loris R. & Kursula P. (2021) Structure of the complete dimeric human GDAP1 core domain provides insights into ligand binding and clustering of disease mutations. Front Mol Biosci. 7: 631232. doi: 10.3389/fmolb.2020.631232.

  • Myllykoski M., Sutinen A., Koski K., Kallio J.P., Raasakka A., Myllyharju J.,  Wierenga R.K. & Koivunen P. (2021) Structure of transmembrane prolyl 4-hydroxylase reveals unique organization of EF and dioxygenase domains. J Biol Chem. 296: 100197. doi: 10.1074/jbc.RA120.016542.

2020

  • Raasakka A. & Kursula P. (2020) How Does Protein Zero Assemble Compact Myelin? Cells 9(8): 1832. doi: 10.3390/cells9081832. Del av spesialutgaven "Schwann Cells: From Formation to Clinical Significance". Oversiktsartikkel.

  • Ruskamo S.*, Krokengen O.C.*, Kowal J., Nieminen T., Lehtimäki M., Raasakka A., Dandey V., Vattulainen I., Stahlberg H. & Kursula P. (2020) Cryo-EM, X-ray diffraction, and atomistic simulations reveal determinants for the formation of a supramolecular myelin-like proteolipid lattice. J Biol Chem. 295: 8692-8705. doi: 10.1074/jbc.RA120.013087.

  • Lillebostad P.A.G.*, Raasakka A.*, Hjellbrekke S.J., Patil S., Røstbø T., Hollås H., Sakya S.A., Szigetvari P.D., Vedeler A. & Kursula P. (2020) Structure of the ALS Mutation Target Annexin A11 Reveals a Stabilising N-Terminal Segment. Biomolecules 10(4): 660. doi: 10.3390/biom10040660. Del av spesialutgaven "Metal Binding Proteins 2020".

  • Raasakka A. & Kursula P. (2020) Flexible Players within the Sheaths: The Intrinsically Disordered Proteins of Myelin in Health and Disease. Cells 9(2): 470. doi: 10.3390/cells9020470. Del av spesialutgaven "Structure and Function of Healthy and Diseased Myelin". Oversiktsartikkel.

2019

  • Raasakka A. & Kursula P. (2019) Stability and flexibility of full-length human oligodendrocytic QKI6. BMC Res Notes. 12: 609. doi: 10.1186/s13104-019-4629-x.

  • Raasakka A., Ruskamo S., Barker R., Krokengen O.C., Vatne G.H., Kristiansen C.K., Hallin E.I., Skoda M.W.A., Bergmann U., Wacklin-Knecht, H., Jones N.C., Hoffmann S.V & Kursula P. (2019) Neuropathy-related mutations alter the membrane binding properties of the human myelin protein P0 cytoplasmic tail. PLoS ONE 14(6): e0216833. doi: 10.1371/journal.pone.0216833.

  • Raasakka A., Linxweiler H., Brophy P.J., Sherman D.L. & Kursula P. (2019) Direct binding of the flexible C-terminal segment of periaxin to β4 integrin suggests a molecular basis for CMT4F. Front Mol Neurosci. 12: 84. doi: 10.3389/fnmol.2019.00084.

  • Raasakka A., Jones N.C., Hoffmann S.V. & Kursula P. (2019) Ionic strength and calcium regulate the membrane interactions of myelin basic protein and the cytoplasmic domain of myelin protein zero. Biochem Biophys Res Commun. 511(1): 7-12. doi: 10.1016/j.bbrc.2019.02.025.

  • Raasakka A., Ruskamo S., Kowal J., Han H., Baumann A., Myllykoski M., Fasano A., Rossano R., Riccio P., Bürck J., Ulrich A.S., Stahlberg H. & Kursula P. (2019) Molecular structure and function of myelin protein P0 in membrane stacking. Sci Rep. 9: 642. doi: 10.1038/s41598-018-37009-4.

2018

  • Laulumaa S., Nieminen T., Raasakka A., Krokengen O.C., Safaryan A., Hallin E.I., Brysbaert G., Lensink M.F., Ruskamo S., Vattulainen I. & Kursula P. (2018) Structure and dynamics of a human myelin protein P2 portal region mutant indicate opening of the β barrel in fatty acid binding proteins. BMC Struct Biol. 18: 8. doi: 10.1186/s12900-018-0087-2.

  • Raasakka A.*, Mahootchi E.*, Winge I., Luan W., Kursula P. & Haavik J. (2018) Structure of the mouse acidic amino acid decarboxylase GADL1. Acta Cryst. F74(1): 65-73. doi: 10.1107/S2053230X17017848.

2017

  • Ruskamo S., Nieminen T., Kristiansen C.K., Vatne G.H., Baumann A., Hallin E.I., Raasakka A., Joensuu P., Bergmann U., Vattulainen I. & Kursula P. (2017) Molecular mechanisms of Charcot-Marie-Tooth neuropathy linked to mutations in human myelin protein P2. Sci Rep. 7: 6510. doi: 10.1038/s41598-017-06781-0.

  • Raasakka A., Ruskamo S., Kowal J., Barker R., Baumann A., Martel A., Tuusa J., Myllykoski M., Bürck J., Ulrich A.S., Stahlberg H. & Kursula P. (2017) Membrane Association Landscape of Myelin Basic Protein Portrays Formation of the Myelin Major Dense Line. Sci Rep. 7: 4974. doi: 10.1038/s41598-017-05364-3.

  • Muruganandam G., Raasakka A., Myllykoski M., Kursula I. & Kursula P. (2017) Structural similarities and functional differences clarify evolutionary relationships between tRNA healing enzymes and the myelin enzyme CNPase. BMC Biochem. 18: 7. doi: 10.1186/s12858-017-0084-2.

  • Tuusa J., Raasakka A., Ruskamo S. & Kursula P. (2017) Myelin-derived and putative molecular mimic peptides share structural properties in aqueous and membrane-like environments. Mult Scler Demyelinating Disord. 2: 4. doi: 10.1186/s40893-017-0021-7.

  • Snaidero N., Velte C., Myllykoski M., Raasakka A., Ignatev A., Werner H.B., Erwig M.S., Möbius W., Kursula P., Nave K.A. & Simons M. (2017) Antagonistic functions of MBP and CNP establish cytosolic channels in central nervous system myelin. Cell Rep. 18(2): 314-323. doi: 10.1016/j.celrep.2016.12.053.

2016

  • Myllykoski M., Seidel L., Muruganandam G., Raasakka A., Torda A.E. & Kursula P. (2016) Structural and functional evolution of 2’,3’-cyclic nucleotide 3’-phosphodiesterase. Brain Res. 1641(Pt A): 64-78. doi: 10.1016/j.brainres.2015.09.004. Del av spesialutgaven "Evolution of Myelin". Oversiktsartikkel.

2015

  • Raasakka A., Myllykoski M., Laulumaa S., Lehtimäki M., Härtlein M., Moulin M., Kursula I. & Kursula P. (2015) Determinants of ligand binding and catalytic activity in the myelin enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase. Sci Rep. 5: 16520. doi: 10.1038/srep16520.

  • Laulumaa S., Blakeley M.P., Raasakka A., Moulin M., Härtlein M. & Kursula P. (2015) Production, crystallization, and neutron diffraction of fully deuterated human myelin peripheral membrane protein P2. Acta Cryst. F71(11): 1391-1395. doi: 10.1107/S2053230X15017902.

  • Chukhlieb M., Raasakka A., Ruskamo S. & Kursula P. (2015) The N-terminal cytoplasmic domain of neuregulin 1 type III is intrinsically disordered. Amino Acids 47(8): 1567-1577. doi: 10.1007/s00726-015-1998-x.

2014

  • Raasakka A. & Kursula P. (2014) The myelin membrane-associated enzyme 2′,3′-cyclic nucleotide 3′-phosphodiesterase: on a highway to structure and function. Neurosci Bull. 30(6): 956-966. doi: 10.1007/s12264-013-1437-5. Oversiktsartikkel.

2013

  • Myllykoski M.*, Raasakka A.*, Lehtimäki M., Han H., Kursula I. & Kursula P. (2013) Crystallographic analysis of the reaction cycle of 2’,3’-cyclic nucleotide 3’-phosphodiesterase, a unique member of the 2H phosphoesterase family. J Mol Biol. 425(22): 4307-4322. doi: 10.1016/j.jmb.2013.06.012.

2012

  • Myllykoski M., Raasakka A., Han H. & Kursula P. (2012) Myelin 2’,3’-cyclic nucleotide 3’-phosphodiesterase: Active-site ligand binding and molecular conformation. PLoS ONE 7(2): e32336. doi: 10.1371/journal.pone.0032336.

Konferanseabstrakter

  • Raasakka A., Han H., Myllykoski M. & Kursula P. (2018) Flexibility of the Myelin Scaffolding Protein Periaxin. Biophys J. 114(3): S1,407a. doi: 10.1016/j.bpj.2017.11.2254. (62nd Annual Meeting of the Biophysical Society, San Francisco, CA, 17. - 21.2.2018)

  • Chukhlieb M., Han H., Myllykoski M., Laulumaa S., Raasakka A., Ruskamo S., Wang C. & Kursula P. (2013) Membrane Interactions, Intrinsic Disorder, and Unknown Functions of Myelin Proteins. Biophys J. 104(2): S1,548a. doi: 10.1016/j.bpj.2012.11.3036. (57th Annual Meeting of the Biophysical Society, Philadelphia, PA, 2. - 6.2.2013)

Avhandlinger

  • Raasakka A. (2019) The Characterization of Disordered Membrane-Binding Proteins of Myelin: A Biophysical Approach. Grad: Philosophiae Doctor (PhD), Universitetet i Bergen, Norge. ISBN: 978-82-308-3520-3.

  • Raasakka A. (2012) Structural Enzymology of 2′,3′-Cyclic Nucleotide 3′-Phosphodiesterase. Grad: Mastergrad i naturvitenskap (MSc), Oulun yliopisto, Finland.

  • Raasakka A. (2010) TNF-α ja etanersepti - sytokiini ja sen inhibiittori. Grad: Bachelorgrad i naturvitenskap (BSc), Oulun yliopisto, Finland.

Nyheter og andre publikasjoner

  • Protein-Lipid Interactions: Advanced Experimental and Computational Tools. Kursrapport i NBS Nytt 3/2017: 30-31.

Posters

  • Raasakka A., Ruskamo S., Krokengen O.C., Schneider R. & Kursula P. Structural biology of the myelin membrane: Unraveling the folding of myelin proteins in health and disease. 18th International Conference on Chiroptical Spectroscopy. 24. – 27.7.2022, New York City, NY, USA.

  • Kind L., Raasakka A., Njølstad P.R., Kursula P. & Arnesen, T. Biophysical and functional studies on the transactivation domain of HNF-1A: Finding order in disorder? FEBS 2022 Advanced Course: Lost in Integration - Probing Biomolecules with Electrons, Photons, Neutrons and Magnetic Spins. 9. – 15.5.2022, Spetses, Greece.

  • Kind L., Molnes J., Raasakka A., Colclough K., Saint-Martin C., Bellanné-Chantelot C., Kursula P., Njølstad P.R. & Arnesen T. Biochemical insights into HNF4A gene regulation and promoter-driven MODY1 diabetes. The Nordic DIABESITY meeting. 9. - 11.3.2022, Solstrand, Norway.

  • Kind L., Raasakka A., Molnes J., Aukrust I., Bjørkhaug L., Njølstad P.R., Kursula P. & Arnesen T. Structural and biophysical characterization of HNF-1A as a tool to study MODY3 diabetes variants. The 66th Biophysical Society Annual Meeting. 19. - 23.2.2022, San Francisco, CA, USA.

  • Kind L., Raasakka A., Njølstad P.R., Kursula P. & Arnesen T. Structural and biochemical characterization of the transcription factor HNF-1A. 6th European Crystallographic School. 4. - 10.7.2021, Budapest, Hungary. (virtual)

  • Houry D., Raasakka A., Bockwoldt M., Niere M., Heiland I., Kursula P. & Ziegler M. Structural insights into the catalytic mechanism of human NAMPT. The NAD+ Metabolism and Signaling Conference. 23. - 28.6.2019, Dublin, Irland.

  • Raasakka A., Myllykoski M., Ruskamo S., Baumann A., Kowal J., Stahlberg H., Eichel M., Werner H., Sherman D., Brophy P. & Kursula P. Intrinsic Disorder and Myelin Protein Complexes. Current Topics in Myelin Research. 28.2. - 2.3.2019, Kassel, Tyskland.

  • Raasakka A., Han H., Myllykoski M. & Kursula P. Flexibility of the Myelin Scaffolding Protein Periaxin. The 62nd Biophysical Society Annual Meeting. 17. - 21.2.2018, San Francisco, CA, De forente stater.

  • Raasakka A. & Kursula P. Molecular Flexibility of Myelin Proteins Enables Tight Packing of Lipid Multilayers. The Kavli Prize Symposium in Nanoscience. Atomic Force Microscopy: Yesterday, Today and Tomorrow. 25.9.2017, Zürich, Sveits.

  • Raasakka A., Baumann A., Barker R., Ruskamo S., Kowal J., Myllykoski M., Bürck J., Stahlberg H. & Kursula P. Myelin Basic Protein - Unraveling Lipid Interactions and Membrane Insertion. 7th Annual BioStruct Conference. 25. - 28.8.2016, Jægtvolden, Norge.

  • Raasakka A., Myllykoski M., Ruskamo S., Laulumaa S. & Kursula P. Exploring the Many Proteins of Myelin - Structural Enzymology and Membrane Stacking in vitro. HERCULES Specialized Course HSC16: Non-atomic resolution scattering in biology and soft matter. 15. - 19.9.2014, Grenoble, Frankrike.

  • Raasakka A.*, Laulumaa S.*, Chukhlieb M., Han H., Muruganandam G., Myllykoski M., Ruskamo S. & Kursula P. Structural Biology of the Myelin Membrane - A Biophysical Approach to a Biological Problem. Biocenter Oulu Discovery of the Year. 16.12.2014, Uleåborg, Finland.

 

* Likt bidrag     † Tilsvarende forfatter

 

  • Biofysisk og strukturell karakterisering av myelinlignende proteolipidmodelsystemer: Hvordan påvirker proteinuorden og netto elektrisk ladning myelinisering og nervesykdomsutvikling.
  • Protein-membran og protein-protein interaksjoner i myelin: Måling av bindingsaffinitet, kinetikk, støkiometri og struktur.

Faglige interesser

Metoder i strukturell og fysisk biokjemi, metodeutvikling, enzymologi, strukturbiologi, nevrobiokjemi og undervisning.

 

Praktisk erfaring

  • Makromolekylær røntgenkrystallografi
  • Røntgensmåvinkelspredning (SAXS) og -diffraksjon (SAXD)
  • Isotropisk og orientert CD spektroskopi
  • Nøytronreflektometri og nøytronsmåvinkelspredning
  • Synkrotronlyskilder
  • Top-down massespektrometri, peptid fingeravtrykking og H-D veksling (MALDI-TOF, LC-ESI-TOF)
  • Atomkraftmikroskopibasert avbildning
  • Flytavbrudd kinetikk
  • Overflateplasmonresonans (Biacore)
  • Lysspredningsmetoder (SEC-MALS, DLS)
  • UV-vis absorpsjon- og fluorescensmetoder
  • Differanseskanningkalorimetri
  • Fluorescensbasert termisk stabilitetsmetoder (Thermofluor/DSF)
  • Isotermisk titreringskalorimetri
  • Uttrykking av rekombinante proteiner i E. coli og S. cerevisiae
  • Proteinrensing med diverse kromatografiske metoder
  • Molekylærbiologiske metoder (klassisk kloning & mutagenese; Gateway kloning)
  • Windows & Linux operativsystemer; MS Office; GraphPad Prism; ATSAS; ScÅtter; XDS; Phenix; PyMOL

 

Utdanning

  • PhD, 2019, Institutt for biomedisin, Universitetet i Bergen, Norge 
  • MSc, 2012, Biokemian laitos, Oulun yliopisto, Finland
  • BSc, 2011, Biokemian laitos, Oulun yliopisto, Finland

 

Forskningshistorie

  • Department of Neurosurgery, Stanford University, CA, USA (2022–)
  • Institutt for biomedisin, Universitetet i Bergen, Norge (2015–)
  • Biokemian ja molekyylilääketieteen tiedekunta, Oulun yliopisto, Finland (2013–2015)
  • Centre for Structural Systems Biology, Helmholtz-Zentrum für Infektionsforschung (CSSB-HZI), Hamburg, Tyskland (2012)
  • Biokemian laitos, Oulun yliopisto, Finland (2008, 2010–2012)

 

Korte forskningsbesøk

  • Equipe Biologie Structurale Integrative (ERL 9002 CNRS), Université de Lille, Frankrike (2019)
  • Deutsches Elektronen-Synchrotron (DESY), Hamburg, Tyskland (2012)

Forskergrupper

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