Hjem
Kari Espolin Fladmarks bilde

Kari Espolin Fladmark

Professor
  • E-postKari.Fladmark@uib.no
  • Telefon+47 55 58 45 14
  • Besøksadresse
    Thormøhlens gate 53 A/B
  • Postadresse
    Postboks 7803
    5020 Bergen

 

Vår forskningsgruppe prøver å forstå hvordan stress fører til celledød og hvilke mekansimer cellene har for å beskytte seg selv og omliggende celler. Vi bruker zebrafisk som in vivo modell for å studere signaler som fører til celledød og beskyttelse. Zebrafisk er blitt en svært populær modell for menneskelig sykdommer. Zebrafiskens utvikling og organer er like våre og sykdomsrelaterte menneskelige gener finnes som funksjonelle gener også i zebrafisk.

Vitenskapelig artikkel
  • Vis forfatter(e) 2020. The Inflammatory Cytokine IL-3 Hampers Cardioprotection Mediated by Endothelial Cell-Derived Extracellular Vesicles Possibly via Their Protein Cargo. Cells. 1-21.
  • Vis forfatter(e) 2019. Dysregulation in the Brain Protein Profile of Zebrafish Lacking the Parkinson’s Disease-Related Protein DJ-1. Molecular Neurobiology. 1-17.
  • Vis forfatter(e) 2018. Astroglial DJ-1 over-expression up-regulates proteins involved in redox regulation and is neuroprotective in vivo. Redox Biology. 237-247.
  • Vis forfatter(e) 2016. Quantitative proteomics analysis of zebrafish exposed to sub-lethal dosages of β-methyl-amino-L-alanine (BMAA). Scientific Reports.
  • Vis forfatter(e) 2015. The N-terminal acetyltransferase Naa10 is essential for zebrafish development. Bioscience Reports. 10 sider.
  • Vis forfatter(e) 2015. Algal toxin azaspiracid-1 induces early neuronal differentiation and alters peripherin isoform stoichiometry. Marine Drugs. 7390-7402.
  • Vis forfatter(e) 2013. Identification of dynamic changes in proteins associated with the cellular cytoskeleton after exposure to okadaic acid. Marine Drugs. 1763-1782.
  • Vis forfatter(e) 2013. Comparative analysis of IgM sub-variants in salmonid fish and identification of a residue in mu 3 which is essential for MAb4C10 reactivity. Fish and Shellfish Immunology. 667-672.
  • Vis forfatter(e) 2012. Nodularin exposure induces SOD1 phosphorylation and disrupts SOD1 co-localization with actin filaments. Toxins. 1482-1499.
  • Vis forfatter(e) 2012. Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae. Journal of Applied Ichthyology. 477-487.
  • Vis forfatter(e) 2012. Ectopic expression of Flt3 kinase inhibits proliferation and promotes cell death in different human cancer cell lines. Cell Biology and Toxicology. 201-212.
  • Vis forfatter(e) 2012. Dietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a comparative 2D-DIGE study. Marine Biotechnology. 643-654.
  • Vis forfatter(e) 2010. Increased interaction between DJ-1 and the Mi-2/nucleosome remodelling and deacetylase complex during cellular stress. Proteomics. 1494-1504.
  • Vis forfatter(e) 2009. Proteomic response of human neuroblastoma cells to azaspiracid-1. Journal of Proteomics. 695-707.
  • Vis forfatter(e) 2009. Ligand-induced Flt3-downregulation modulates cell death associated proteins and enhances chemosensitivity to idarubicin in THP-1 acute myeloid leukemia cells. Leukemia Research. 276-287.
  • Vis forfatter(e) 2009. Acyloxymethyl esterification of Nodularin-R and Microcystin-LA produces inactive protoxins that become reactivated and produce apoptosis inside intact cells. Journal of Medicinal Chemistry. 5758-5762.
  • Vis forfatter(e) 2008. Identification of protein interaction partners by immunoprecipitation: Possible pitfalls and false positives. Experimental and Clinical Hepatology. 16-22.
  • Vis forfatter(e) 2008. Identification of a novel phosphorylation site of acyl-CoA binding protein (ACBP) in nodularin-induced apoptotic hepatocytes. Experimental Cell Research. 2141-2149.
  • Vis forfatter(e) 2007. Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Proteomics. 1702-1718.
  • Vis forfatter(e) 2006. CaM-kinasell-dependent commitment to microcystin-induced apoptosis is coupled to cell budding, but not to shrinkage or chromatin hypercondensation. Cell Death and Differentiation. 1191-1202.
  • Vis forfatter(e) 2004. Caspase-dependent, geldanamycin-enhanced cleavage of co-chaperone p23 in leukemic apoptosis. Leukemia. 1989-1996.
  • Vis forfatter(e) 2003. Changed energy state and increased mitochondrial beta-oxidation rate in liver of rats associated with lowered proton electrochemical potential and stimulated uncoupling protein 2 (UCP-2) expression. Journal of Biological Chemistry. 30525-30533.
  • Vis forfatter(e) 2003. Changed Energy State and Increased Mitochondrial B-Oxidation Rate in Liver of Rats Associated with Lowered Proton Electrochemical Potential and Stimulated Uncoupling Protein 2 (UCP-2) Expression. Journal of Biological Chemistry. 30525-30533.
  • Vis forfatter(e) 2002. Ca2+/calmodulin dependent protein kinase II is required for microcystin-induced apoptosis. Journal of Biological Chemistry. 2804-2811.
  • Vis forfatter(e) 2001. Apoptotic effect of cyanobacterial extract on rat hepatocytes and human lymphocytes. Environmental Toxicology. 225-233.
  • Vis forfatter(e) 2001. Apoptotic effect of cyanobacterial blooms collected from Polish water reservoirs. International Journal of Occupational Medicine and Environmental Health. 335-344.
  • Vis forfatter(e) 2000. An ultrasensitive competitive binding assay for the detection of protein phosphatase directed toxins. Toxicon. 347-360.
  • Vis forfatter(e) 1999. Ultrarapid apoptosis inducted by phosphatase inhibitors. Cell Death Differ. Cell Death and Differentiation. 1099-1108.
  • Vis forfatter(e) 1999. Caspase I related protease inhibition retards the execution of okadaic acid and camptothecin induced apoptosis and PAI-2 selective cleavage, but not commitment to cell death in HL-60 cells. British Journal of Cancer. 1685-1691.
  • Vis forfatter(e) 1999. An ultrasensitive competitive binding assay for the detection of toxins affecting protein phosphatases. Toxicon. 347-360.
  • Vis forfatter(e) 1998. Sensitive detection of apoptogenic toxins in suspension cultures of rat and salmon hepatocytes. Toxicon. 1101-1114.
  • Vis forfatter(e) 1998. Apoptosis induced by microinjection of cytochrome c is caspase-dependent and is inibited by Bcl-2. Cell Death and Differentiation. 660-668.
  • Vis forfatter(e) 1997. Gap junctions and growth control in liver regeneration and in isolated rat hepatocytes. Hepatology. 847-855.
  • Vis forfatter(e) 1997. Fas/APO-1 (CD95)-Induced Apoptosis of Primary Hepatocytes Is Inhibited by cAMP. Biochemical and Biophysical Research Communications - BBRC. 20-25.
  • Vis forfatter(e) 1995. Ala%3%3%5 is essential for high-affinity cAMP-binding of both sites A and B of cAMP-dependent protein kinase type I. FEBS Letters. 291-294.
  • Vis forfatter(e) 1995. ALA-335 is essential for high-affinity cAMP-binding of both sites A and B of cAMP-dependent protein kinase type I. FEBS Letters. 291-294.
Rapport
  • Vis forfatter(e) 2006. Shellfish toxin azaspiracid-1 induced changes in cytoskeletal proteins in neuronal cells. .
  • Vis forfatter(e) 2006. Oocyte development in Atlantic cod (Gadus morhua L.): Trypsin-like specific activity, capacity for protein synthesis and proteomics. .
Faglig foredrag
  • Vis forfatter(e) 2016. Functional Analysis of the DJ-1 Protein Using a CRISPR/Cas9 Knockout of the Park7 Gene in Zebrafish.
Vitenskapelig foredrag
  • Vis forfatter(e) 2018. Role of the Parkinsons Disease-related protein DJ-1 in redox control and neuronal protection.
  • Vis forfatter(e) 2017. Using zebrafish to understand BMAA-induced neurotoxicity and its relevance to neurodegeneration.
  • Vis forfatter(e) 2011. The muscle proteome of growing fish is affected by dietary lysine.
  • Vis forfatter(e) 2009. Saxitoxin biosynthesis in organisms from two kingdoms - horizontal gene transfer or parallel evolution?
  • Vis forfatter(e) 2008. Identification of DJ1 interaction partners using a quantitative proteomics tool.
  • Vis forfatter(e) 2006. Late breaker: Revealing the secreted proteome of Mycobacterium tuberculosis H37Rv.
  • Vis forfatter(e) 2003. cAMP induced protein phosphorylation in acute myelogenous leukemia cells.
  • Vis forfatter(e) 2002. Protein phosphatases as targets in natural compounds-induced apoptosis.
  • Vis forfatter(e) 2002. Bioactive compounds from cyanobacteria.
  • Vis forfatter(e) 1998. Ultrarapid apoptosis induction in a variety of cell types injected with phosphatase inhibitors is mediated through Ca2 /calmodulin dependent protei kinase activity.
  • Vis forfatter(e) 1998. Marine cyanobacteria as a source for bioactive (apoptosis-modifying) compounds with potential as cell biology reagents and drugs.
  • Vis forfatter(e) 1998. Distinct apoptotic death forms. Distinct pathways and functions?
Fagbok
  • Vis forfatter(e) 2020. DJ-1 in astrocytic neuroprotection to oxidative stress. Elsevier.
Mastergradsoppgave
  • Vis forfatter(e) 2019. Impact of cysteine 106 oxidation on function and localisation of the Parkinson’s disease-related protein DJ-1.
  • Vis forfatter(e) 2017. The protective function of L-serine in a zebrafish model of B-methylamino-L-alanine induced toxicity.
  • Vis forfatter(e) 2015. Neuronal DJ-1 functions in a zebrafish model for Parkinson’s Disease.
  • Vis forfatter(e) 2015. Effects of β-methylamino-L-alanine (BMAA)on brain-derived cell lines and zebrafish.
  • Vis forfatter(e) 2013. Mechanistic studies of Atlantic cod responses to alkyl-substituted naphthalene and phenanthrene.
  • Vis forfatter(e) 2012. Role of the Parkinson´s disease-related protein DJ-1 in oxidative stress-induced cell death.
  • Vis forfatter(e) 2011. DJ-1 expression and function in AML cell lines.
  • Vis forfatter(e) 2009. Apoptosis-related post translational modifications of endoplasmic reticulum protein-57 (ERp57).
  • Vis forfatter(e) 2007. A study on the Parkinson’s disease related DJ-1 protein. Possible function and involvement in algal toxin-induced apoptosis.
  • Vis forfatter(e) 2005. Role of Cu/Zn superoxide dismutase (SOD1) in phosphatase inhibitor-induced apoptosis.
Populærvitenskapelig artikkel
  • Vis forfatter(e) 2003. PROBE-nasjonal plattform i proteomikk. NBS-nytt. 23-24.
  • Vis forfatter(e) 2001. Biologisk baserte blåskjelltester (BBB). Fiskehelse. 26-33.
Doktorgradsavhandling
  • Vis forfatter(e) 2019. Functional importance of the Parkinson’s disease-related protein DJ-1.
  • Vis forfatter(e) 2015. Cellular responses to algal toxins.
  • Vis forfatter(e) 2011. The cellular distress proteome. A quantitative study of oxidativily stressed cells.
  • Vis forfatter(e) 1998. Hepatocyte proliferation and apoptosis-regulation by cell communication and phosphorylation. -.
Intervju
  • Vis forfatter(e) 2019. Denne fisken vil trolig få Parkinson.
  • Vis forfatter(e) 2016. Studerer sebrafisk for å finne Parkinsonsvar.
  • Vis forfatter(e) 2016. Sebrafrisk Glem laboratorierotta. Nå er sebrafisken forskernes nye favoritt i kampen mot både fysiske og psykiske sykdommer.
Programdeltagelse
  • Vis forfatter(e) 2015. Death - a series about life.
Vitenskapelig Kapittel/Artikkel/Konferanseartikkel
  • Vis forfatter(e) 2014. Proteomic tools to elucidate the molecular action of micro-algal toxins. 21 sider.
Sammendrag/abstract
  • Vis forfatter(e) 2018. The protein cargo of endothelial cell-derived extracellular vesicles released in response to IL-3 identifies the Wnt signaling pathway as a relevant mediator of inflammation. Journal of Extracellular Vesicles.
  • Vis forfatter(e) 2006. Proteomic tissue imaging for rapid brain biomarker discovery. European Journal of Neurology. 42-42.
Poster
  • Vis forfatter(e) 2018. naa10 knockdown and NatA inhibition point to role for the NatA complex in zebrafish dorsoventral axis formation .
  • Vis forfatter(e) 2018. Functional Analysis of the DJ-1 Protein Using a CRISPR/Cas9 Knockout of the Park7 Gene in Zebrafish.
  • Vis forfatter(e) 2016. A protective function of increased glial DJ-1 expression in a zebrafish model of Parkinson’s disease.
  • Vis forfatter(e) 2015. The N-terminal acetyltransferase Naa10 is essential for zebrafish development.
  • Vis forfatter(e) 2015. High-definition quanlitative and quantitative proteomics of a Parkinson´s disease zebrafish model.
  • Vis forfatter(e) 2014. Functional Mechanisms Behind the Oxidative Stress Protective Action of DJ-1.
  • Vis forfatter(e) 2013. Proteome profiling of zebrafish embryos with glial specific over-expression of the Parkinson’s disease-related protein DJ-1.
  • Vis forfatter(e) 2011. Growth and skeletal muscle proteome of juvenile zebrafish is affected by a dietary lysine.
  • Vis forfatter(e) 2008. The hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus; a proteomics study on the shift between chemoorganoheterotrophic and chemilithoautotrophic growth.
  • Vis forfatter(e) 2008. Protein expression changes in human neuroblastoma cells treated with azaspiracid-1.
  • Vis forfatter(e) 2008. DJ-1 interacts with the chromatin remodelling complex NuRD to regulate apoptotic cell death.
  • Vis forfatter(e) 2006. Oocyte development in Atlantic cod (Gadus morhua L.): Trypsin-like specific activity, capacity for protein synthesis and proteomics.
  • Vis forfatter(e) 2006. Comparing the protein identifications obtained using two-dimensional gel electrophoresis or liquid chromatography mass spectrometry on the secreted proteome of Mycobacterium tuberculosis.
  • Vis forfatter(e) 2003. Differential Expression of Splicing factor SR proteins and Their phosphorylation in Cell Stress leading to Apoptosis.
  • Vis forfatter(e) 2002. Protein phosphatase 2A as a receptor for diarrhetic shellfish poisoning (DSP) toxins- Development of a sensitive in vitro assay.
  • Vis forfatter(e) 2002. Phosphorylated targets in ultrarapidly induced apoptosis.
  • Vis forfatter(e) 1997. Detedtion of toxins from marine cyanobacteria based on their affinity for protein phosphatase 2A and their ability to induce apoptosis in salmon hepatocytes.
Vitenskapelig oversiktsartikkel/review
  • Vis forfatter(e) 2017. European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biology. 94-162.
  • Vis forfatter(e) 2015. Redox control of protein degradation. Redox Biology. 409-420.
  • Vis forfatter(e) 2006. Proteomics approaches to elucidate oncogenic tyrosine kinase signaling in myeloid malignancies. Current Pharmaceutical Biotechnology. 185-198.
  • Vis forfatter(e) 2006. Algal toxins as guidance to identify phosphoproteins with key roles in apoptotic cell death. Current Pharmaceutical Biotechnology. 209-215.

Se fullstendig oversikt over publikasjoner i CRIStin.

Dysregulation in the brain protein profile of zebrafish lacking the Parkinson’s disease related protein DJ-1. Edson, A.J., Hushagen, H.A., Frøyset, AK., Elda, I., Khan, E.A., Di Stefano, A., Fladmark, K.E. (2019)  Mol. Neurobiol. DOI: 10.1007/s12035-019-01667-w

Astroglial DJ-1 over-expression up-regulates proteins involved in redox regulation and is neuroprotective in vivo. Frøyset AK Edson AJ, Gharbi N, Khan EA, Dondorp D, Bai Q, Tiraboschi E, Suster ML, Connolly JB, Burton EA, and Fladmark KE. (2018) Redox Biol. 16:237-247.

Quantitative proteomics analysis of zebrafish exposed to sub-lethal dosages of BMAA. Frøyset AK, Khan EA, Fladmark KE (2016)  Sci Reports 6:29631.

Redox control of protein degradation. Pajares M, Jimineze-Moreno N, Dias I, Debelec B, Vucetic M, Fladmark KE, Basaga H, Ribaric S, Milisav I, Cuadrado A (2015)  RedOx Biology 6:409-20.

The Algal Toxin Azaspiracid-1 Induces an Early Neuronal Differentiation and Alters Peripherin Isoform Stoichiometry . Hjørnevik LV , Frøyset AK, Grønset TA, Rungruangsak-Torrissen K,  and Fladmark KE (2015)  Marine Drugs 13, 7390-7402.

The N-terminal acetyltransferase Naa10 is essential for zebrafish development. Ree RM, Myklebust L, Thiel P, Foyn H, Fladmark KE, and Arnesen T (2015)  Bioscience Reports Aug. 6

Proteomic tools to elucidate the molecular action of microalgal toxins. In Toxins and biologically active compounds from microalgae. Ed Rossini GP Fladmark KE (2014) (Book chapter)

Identification of dynamic changes in proteins associated with the cellular cytoskeleton after exposure to okadaic acid. Opsahl JA, Ljostveit S, Solstad T, Risa K, Roepstorff P, and Fladmark KE (2013) Marine Drugs11:1763-82.

Comparative analysis of IgM sub-variants in salmonid fish and identification of a residue in m3 which is essential for MAb4C10 reactivity. KamilA, RaaeA, Fjelldal PG, Koppang EO, Fladmark KE, Hordvik I (2013)Fish and Shellfish Immunology 34: 667-72.

Nodularin exposure induces SOD1 phosphorylation and distrupts SOD1 co-localization with actin. Hjørnevik LV, Fismen L, Young FM, Solstad T , Fladmark KE (2012) . Toxins 4:1482-1499.

Dietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a comparative 2D-DIGE study. de Vareilles M, Conceição L, Gómez-Requeni P, Kousoulaki K, Richard N, Rodrigues P, Fladmark, KE, Rønnestad I, (2012) Marine Biotechnology 14:643-654.

Impact of dietary protein hydrolysates on skeleton quality and proteome in Diplodus sargus larvae. de Vareilles M, Richard N, Gavaia PJ, Silva TS., Cordeiro O, Guerreiro I, Yúfera M, Batista I, Pires C, Pousão-Ferreira P, Rodrigues PM, Rønnestad I, Fladmark KE, Conceição LEC (2012) J Appl Ichthyol 28: 477-87.

Ectopic expression of Flt3 kinase inhibits proliferation and promotes cell death in different human cancer cell lines Oveland E, Wergeland L, Hovland R, Lorens JB, Gjertsen BT, Fladmark KE. Cell Biol Toxicol. 2012 Mar 16.

Increased interaction between DJ-1 and the Mi-2/ nucleosome remodelling and deacetylase complex during cellular stress. Opsahl JA, Hjornevik LV, Bull VH, Fismen L, Froyset AK, Gromyko D, Solstad T, Fladmark KE. Proteomics. 2010;10(7):1494-504.

Acyloxymethyl Esterification of Nodularin-R and Microcystin-LA Produces Inactive Protoxins that Become Reactivated and Produce Apoptosis inside Intact Cells. Herfindal L, Kasprzykowski F, Schwede F, Lankiewicz L, Fladmark KE, Lukomska J, Wahlsten M, Sivonen K, Grzonka Z, Jastorff B, Døskeland SO J Med Chem 2009 Epub 2009 aug.

Proteomic response of human neuroblastoma cells to azaspiracid-1. Kellmann R, Schaffner CA, Grønset TA, Satake M, Ziegler M, Fladmark KE. J Proteomics. 2009 May 2;72(4):695-707. Epub 2009 Mar 5.

Ligand-induced Flt3-downregulation modulates cell death associated proteins and enhances chemosensitivity to idarubicin in THP-1 acute myeloid leukemia cells. Oveland E, Gjertsen BT, Wergeland L, Selheim F, Fladmark KE, Hovland R. Leuk Res. 2009 Feb;33(2):276-87. Epub 2008 Aug 8.

Identification of a novel phosphorylation site of acyl-CoA binding protein (ACBP) in nodularin-induced apoptotic hepatocytes. Solstad T, Fismen L, Garberg H, Fladmark KE. Exp Cell Res. 2008 Jun 10;314(10):2141-9. Epub 2008 Mar 30.

Comprehensive analysis of exported proteins from Mycobacterium tuberculosis H37Rv. Målen H, Berven FS, Fladmark KE, Wiker HG. Proteomics. 2007 May;7(10):1702-18.

CaM-kinaseII-dependent commitment to microcystin-induced apoptosis is coupled to cell budding, but not to shrinkage or chromatin hypercondensation. Krakstad C, Herfindal L, Gjertsen BT, Bøe R, Vintermyr OK, Fladmark KE, Døskeland SO. Cell Death Differ. 2006 Jul;13(7):1191-202. Epub 2005 Nov 25.

Algal toxins as guidance to identify phosphoproteins with key roles in apoptotic cell death. Solstad T, Fladmark KE. Curr Pharm Biotechnol. 2006 Jun;7(3):209-15. Review.

Proteomics approaches to elucidate oncogenic tyrosine kinase signaling in myeloid malignancies. Oveland E, Fladmark KE, Wergeland L, Gjertsen BT, Hovland R. Curr Pharm Biotechnol. 2006 Jun;7(3):185-98. Review.

Pågående prosjekter:

  • "Role of phosphorylation in regulation of redox-mediated events in apoptotic cell death" (FUGE)
  • "Nordic Network in proteomics" (NordForsk) NORPRO
  • "Kompetanseoppbygging i kvantitativ proteomikk" (Meltzer)
  • "Nordic network in quantitative proteomics" (NordForsk) Q-NORDIC

Dr. scient, University of Bergen. “Hepatocyte proliferation and apoptosis: regulation by cell communication and phosphorylation” (1998)

Forskergrupper