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Nils Åge Frøysteins bilde

Nils Åge Frøystein

Førsteamanuensis
  • E-postNils.Froystein@uib.no
  • Telefon+47 55 58 35 46
  • Besøksadresse
    Realfagbygget, Allégt. 41
  • Postadresse
    Postboks 7803
    5020 BERGEN

Forskninga mi dreiar seg for ein stor del om bruk av høgoppløysande multikjerne (1H, 13C, 15N) og multidimensjonal (2D/3D) NMR-spektroskopi av isotopisk anrika (13C, 15N) protein i løysing og 2D NMR-spektroskopi av proteinbindande peptid. NMR-spektrene kan gje informasjon om struktur av molekyl, dynamiske prosessar (utveksling) og vekselverknader (binding) mellom til dømes store biomolekyl og mindre ligand molekyl. Eg er mest interessert i dei metodiske sidene av NMR-spektroskopi, og eg arbeider med NMR-spektroskopi innanfor mange bruksområde i kjemi.

Undervisning

Eg underviser følgjande kurs:

KJEM100 / Kjemi i naturen

KJEM306 / NMR-spektroskopi II

Masteroppgåver

NMR-spektrosopi av protein og peptid: Masteroppgåver blir tilbudt innanfor bruk av høgoppløysande multikjerne (1H, 13C, 15N og andre) og multidimensjonal (1D, 2D og 3D) NMR-spektroskopi brukt på protein, peptid og mindre molekyl som vekselverkar med protein. NMR-spektroskopi kan gje viktig informasjon om funksjon og virkemåte til/mekanismar for protein på molekylnivå i løysing. Dette kan vera strukturell informasjon for aktive sete, binding av peptid og småmolekylar (substrat, kofaktorar, inhibitorar og medisinmolekyl) og dynamiske prosessar (utveksling av små ligandmolekyl). Valg av NMR-metodar og etterprosesseringsprosedyrar vil vera sterkt avhengige av kva for type informasjon ein er ute etter i kvart tilfelle.

Denne forskinga er eit samarbeid med institutt for Molekylærbiologi.

Vitenskapelig artikkel
  • 2019. A two-step telescoped continuous flow switchable process leading to nitriles, diaziridine or hydrazine derivatives. Reaction Chemistry & Engineering. 41-51.
  • 2018. Steric factors on unsymmetrical O-hydroxyaryl N-Heterocyclic carbene ligands prevailing the stabilization of single stereoisomer of bis-ligated titanium complexes. Journal of Organometallic Chemistry. 106-116.
  • 2014. Mutation of the highly conserved ser-40 of the HIV-1 p6 gag protein to phe causes the formation of a hydrophobic patch, enhances membrane association, and polyubiquitination of gag. Viruses. 3738-3765.
  • 2013. Screening of the relaxivity of gadolinium-loaded periodic mesoporous silica functionalized by means of soft metalorganic silylamide grafting. European Journal of Inorganic Chemistry (EurJIC). 5969-5979.
  • 2012. Thermal stability of chicken brain alpha-spectrin repeat 17: a spectroscopic study. Journal of Biomolecular NMR. 71-83.
  • 2012. Human Protein N-terminal Acetyltransferase hNaa50p (hNAT5/hSAN) Follows Ordered Sequential Catalytic Mechanism (combined kinetic and NMR study). Journal of Biological Chemistry. 10081-10088.
  • 2009. Synthesis of a new bidentate NHC–Ag(I) complex and its unanticipated reaction with the Hoveyda–Grubbs first generation catalyst. Tetrahedron. 7186-7194.
  • 2009. Cytotoxic trans-oriented iminoether platinum complexes - Kinetics of binding to DNA oligonucleotides determined by 15-N NMR spectroscopy. Inorganica Chimica Acta. 907-914.
  • 2008. Characterization and reactivity of peralkylated Ln(II)Al(III) heterobimetallic complexes. Dalton Transactions. 1899-1907.
  • 2007. Homoleptic rare-earth metal(III) tetramethylaluminates: Structural chemistry, reactivity, and performance in isoprene polymerization. Chemistry - A European Journal. 8784-8800.
  • 2006. Crystalline products isolated from solutions with commercially available 2,3-bis(2-pyridyl)pyrazine (dpp) as reactant: Detection of a dimerized form of dpp. Journal of Molecular Structure. 1-17.
  • 2005. Conformational flexibility of alpha-lactalbumin related to its membrane binding capacity. Journal of Molecular Biology (JMB). 1072-1086.
  • 2004. Tetrahydrobiopterin binding to aromatic amino acid hydroxylases. Ligand recognition and specificity. Journal of Medicinal Chemistry. 5962-5971.
  • 2000. Preparation and NMR studies of 3-selenaheptadecanoic and 4-selenaoctadecanoic acid, RSe(CH2)nC(O)OH (R = -C14H29, n = 1 and 2), and their methyl esters. Journal of the Chemical Society. Perkin Transactions 2 (2001). 723-729.
  • 1999. The structural basis of the recognition of phenylalanine and pterin cofactors by phenylalanine hydroxylase. Implications for the catalytic mechanism. Journal of Molecular Biology (JMB). 807-823.
  • 1999. The structural Basis of the Recognition of Phenylalanine and Pterine Cofactors by Phenylalanine Hydroxylase. Implications for the Catalytic Mechanism. Journal of Molecular Biology (JMB). 807-823.
  • 1998. Myricetin 3-rhamnopyranosyl (1-6) galactoside from Nymphaea x marliacea. Phytochemistry. 1997-2000.
  • 1998. Interaction of Manganese (II)Cobalt(II) and Nickel(I) with DNA Oligomers Studied by Proton NMR Spectroscopy. Inorganica Chimica Acta. 41-46.
  • 1996. Mercuration of acetone in the acetone-mercury(II) perchlorate2-('2-pyridyl)quinoxaline(L) system. Polyhedron. 1035-1039.
  • 1994. The binding of mercury(II) to the syntetic oligonucleotide d(CGcGAATTCGCG)%cA %1H adn %1%5N NMR study. Journal of the American Chemical Society. 3240-3250.
  • 1993. Sequence-Selective Metal Ion Binding to DNA Oligonucleotides. Acta Chemica Scandinavica. 649-657.
  • 1992. Copper(II) - Purine Complexes Studied by Proton and Nitrogen-15 Nuclear Magnetic Resonance. Acta Chemica Scandinavica. 446-450.
  • 1991. Solid-Phase Synthesis and Spectroscopic Studies of TRH Analogues Incorporating Cis- and trans-4-Hydroxy-L-Proline. Acta Chemica Scandinavica. 1047-1054.
Vitenskapelig foredrag
  • 2008. NMR kinetic studies using isotope labeled reagents.
  • 1998. NMR study on the conformation of L-phenylalanine and dihydrobiopterin bound to wild-type and mutant forms of recombinant human phenylalanine hydroxylase.
Short communication
  • 2014. Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR. Molecules. 8890-8903.
Hovedfagsoppgave
  • 2004. An NMR study of alpha-lactalbumin: pKa-determinations and structural calculations.
Mastergradsoppgave
  • 2015. PELARGONIDIN-3-GLUCOSIDE: AN APPROACH TO STRUCTURE ELUCIDATION BY NMR - Choosing the right experiments.
Doktorgradsavhandling
  • 1993. Sequence-selective interactions of DNA and metal ions : NMR studies of the interaction of divalent transition metal ions with oligodeoxyribonucleotides.
Vitenskapelig Kapittel/Artikkel/Konferanseartikkel
  • 2009. Sequence-Selective Binding of Transition Metal Complexes to DNA. 28 sider.
  • 1996. NMR studies of oligonucleotide - metal ion complexes. 1 sider.
  • 1996. NMR Studies of Oligonucleotide-Metal Ion Interactions. 19 sider.
  • 1995. NMR spectroscopic structure determination of metal ion - oligonucleotide complexes - the results of sequence-selective binding studies. 8 sider.
Poster
  • 2006. Dissociative versus Associative Alkyl Exchange: An Extended Dynamic NMR Spectroscopic Study of Ln(AlMe4)3.

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