- E-postoda.krokengen@uib.no
- BesøksadresseJonas Lies vei 915009 Bergen
- PostadressePostboks 78045020 Bergen
The research group focuses on the structure, function and dynamics of myelin proteins. Important results have been produced on the structure and function of myelin proteins, as well as protein-membrane interactions and lipid membrane stacking. However, knowledge about molecular structure and interactions in a membrane with a natural myelin biochemical composition is limited.
This project will use structural biology and nanoscience to address the fine molecular details of how myelin proteins interact with each other and with model membranes. It will assess the mechanisms of lipid bilayer stacking by native-like combinations of CNS and PNS myelin proteins and will include both integral and peripheral membrane proteins that stack lipid bilayers spontaneously in vitro. This PhD project will answer questions regarding the effects of myelin proteins on stacking lipid membranes when they are combined, how they affect each other’s properties, how they reside within lipid assemblies, and whether they compete or function in synergy. Examination of structural differences will be conducted and compared to the structures of the individual proteins. Does the protein mixture reveal new properties relative to those already established? Mutated proteins will be used to see effects on stacking of bilayers in protein mixtures. This research will improve our understanding of myelin.
Ved Universitetet i Bergen
2023
- Andre semester medisin- og odontologistudiet (MEDOD2)
- Undervisningsassisent: DNA-kurs
2022
Biokjemi (FARM150)
Undervisningsassistent: ensym-farmasi og ernæring
Første semester medisin- og odontologistudiet (MEDOD1)
Undervisningsassistent: Titreranalyse, enzymkinetikk
Metodar i medisinsk cellebiologi (BMED320)
Veileder for to studenter. 8 uker med laboratoriearbeid
2021
Biokjemi (FARM150)
Undervisningsassistent: ensym-farmasi og ernæring
Proteinvisualisering: Bruk av PyMOL
Opplæring og veiledning av Erasmusstudent: 2 mnd
Første semester medisin- og odontologistudiet (MEDOD1)
Undervisningsassistent: ensymkinetikk
2020
Biokjemi (FARM150)
Undervisningsassistent: ensym-farmasi og ernæring
Første semester medisin- og odontologistudiet (MEDOD1)
Undervisningsassistent: ensymkinetikk
2019
Metodar i medisinsk cellebiologi (BMED320)
Veileder for to studenter. 8 uker med laboratoriearbeid
Ansvar for instrumentene DLS, CD og SEC-MALS. Opplæring for alle nye brukere
- (2023). The intrinsically disordered protein glue of the myelin major dense line: Linking AlphaFold2 predictions to experimental data. Biochemistry and Biophysics Reports. 11 sider.
- (2020). Cryo-EM, X-ray diffraction, and atomistic simulations reveal determinants for the formation of a supramolecular myelin-like proteolipid lattice. Journal of Biological Chemistry. 8692-8705.
- (2019). Neuropathy-related mutations alter the membrane binding properties of the human myelin protein P0 cytoplasmic tail. PLOS ONE. 1-24.
- (2018). Structure and dynamics of a human myelin protein P2 portal region mutant indicate opening of the β barrel in fatty acid binding proteins. BMC Structural Biology.
- (2018). Studying lipid interactions of specific myelin proteins using nanoscaled model membrane-mimics and nanoparticles.
Se fullstendig oversikt over publikasjoner i CRIStin.
Forskningsartikler
2024
- José M. Godoy Muñoz, Lasse Neset, Sigurbjörn Markússon, Sarah Weber, Oda C. Krokengen, Aleksi Sutinen, Eleni Christakou, Andrea J. Lopez Moreno, Clive R. Bramham, Petri Kursula (2024) Structural cahracterization of two nanobodies targeting the ligand-binding pocket of human Arc. PLoS ONE
2023
Oda C. Krokengen, Arne Raasakka, Petri Kursula (2023) The intrinsically disordered protein glue of the myelin major dense line: Linking AlphaFold2 predictions to experimental data. Biochem Biophys Rep. DOI: 10.1016/j.bbrep.2023.101474. PMID: 37153862; PMCID: PMC10160357.
2020
- Salla Ruskamo*, Oda C. Krokengen*, Julia Kowal, Tuomo Nieminen, Mari Lehtimäki, Arne Raasakka, Saara Leulumaa, Venkata P. Dandey, Ilpo Vattulainen, Henning Sthalberg, Petri Kursula (2020) Cryo-EM, X-ray diffraction, and atomistic simulations reveal determinants for the formation of a supramolecular myelin-like proteolipid lattice. JBC. DOI:https://doi.org/10.1074/jbc.RA120.013087
2019
- Arne Raasakka, Salla Ruskamo, Robert Barker, Oda C. Krokengen, Guro H. Vatne, Cecilie K. Kristiansen, Erik I. Hallin, Maximilian W.A. Skoda, Ulrich Bergmann, Hanna Wacklin-Knecht, Nykola C. Jones, Søren V. Hoffmann & Petri Kursula (2019) Neuropathy-related mutations alter the membrane binding properties of the human myelin protein P0 cytoplasmic tail. PLoS ONE. DOI: https://doi.org/10.1371/journal.pone.0216833
2018
- Saara Laulumaa, Tuomo Nieminen, Arne Raasakka, Oda C. Krokengen, Anushik Safaryan, Erik I. Hallin, Guillaume Brysbaert, Marc F. Lensink, Salla Ruskamo, Ilpo Vattulainen & Petri Kursula (2018) Structure and dynamics of a human myelin protein P2 portal region mutant indicate opening of the β barrel in fatty acid binding proteins. BMC Struct. Biol. 18:8. DOI: 10.1186/s12900-018-0087-2
Andre publikasjoner
- Oda C. Krokengen, Petri Kursula (2022) Building the bridge: Early evidence of increased ordered structures when using bicelles as a mimic model for myelin sheath. NBS nytt - tidsrift for norsk biokjemisk selskap. NR 1 - 2022
- Oda C. Krokengen (2019) Nanoteknologi hjelper til å forstå myelin – nervenes isolasjonslag. Naturen 01/2019 DOI:10.18261/issn.1504-3118-2019-01-07
Avhandlinger
- Oda C. Krokengen (2018) Studying lipid interactions of specific myelin proteins using nanoscaled model membrane-mimics and nanoparticles. University of Bergen URL: http://hdl.handle.net/1956/18754
*Delt forfatterskap
Praktisk erfaring
- Small angle X-ray scattering/diffraction (SAXS/SAXD)
- Synchrotron Radiation Circular Dichroism (SRCD)
- Stopped-flow SRCD
- Dynamic light scattering (DLS)
- Transmission electron microscopy (TEM)
- Myelin models mimics: Liposomes and bicelles
- UV-vis and fluorescence spectroscopy
- Differential scanning calorimetry (DSC)
- SEC-MALS
- Differential Scanning Fluorimetry (DSF)
- E. coli expression of recombinant proteins
- Protein purification using chromatographic methods
- Genotyping
- Bacterial transformation
- Windows & macOS; MS Office; GraphPad Prism; ATSAS
Andre verv:
- Valgt inn som medlem av Instituttrådet (2022-2023) for gruppe B (midlertidig akademiske ansatte)
Utdanning
PhD, Ongoing, Insitutt for Biomedisin, Universitetet i Bergen, Norge
MSc, 2019, Insitutt for Biomedisin, Universitetet i Bergen, Norge
BSc, 2016, Det matematisk-naturvitskaplege fakultet, Universitetet i Bergen, Norge