CCBIO Seminar – Bjørn Tore Gjertsen
Velkommen til CCBIOs seminarserie for vårsemesteret 2023. Åpent for alle i auditorium 4, BBB. Foreleser er Bjørn Tore Gjertsen, og emnet er Early response evaluation by single cell signaling profiling in myeloid leukemia. Bli også gjerne med på uformell mingling over pizza i hallen utenfor etter seminaret!
Hovedinnhold
Speaker: Bjørn Tore Gjertsen, CCBIO PI, Professor of Hematology at the Department of Clinical Science at the University of Bergen, Research Chief and Consultant Hematologist at Helse Bergen Health Trust.
Title: Early response evaluation by single cell signaling profiling in myeloid leukemia
Host: Erling Høivik
Where: Auditorium 4, BB-building
When: January 26, 2023 at 14.30-15.30
No registration necessary. Join us also for the informal pizza get-together in the hall following the talk!
Abstract: Aberrant pro-survival signaling is a hallmark of cancer cells, but the response to chemotherapy is poorly understood. We investigate the initial signaling response to standard induction chemotherapy in acute myeloid leukemia (AML) patients, using multi-dimensional mass cytometry. Through supervised and unsupervised machine learning approaches, we find that reduction of extracellular-signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinase (MAPK) phosphorylation in the myeloid cell compartment 24h post-chemotherapy is a significant predictor of patient 5-year overall survival. Validation by RNA sequencing and proteomics show induction of MAPK targets in patients with high phospho-ERK1/2 24h post-chemotherapy. Single cell signaling profiling seems to be a valuable tool for early response evaluation in AML. We compare this response to chemotherapy with targeted kinase inhibitor therapy in chronic myeloid leukemia and discuss the potential of functional signaling analyses in precision oncology diagnostics.
References:
- Gausdal G, Gjertsen BT, McCormack E, et al. Abolition of stress-induced protein synthesis sensitizes leukemia cells to anthracycline-induced death. Blood. 2008;111(5):2866-2877. doi:10.1182/blood-2007-07-103242
- Gullaksen SE, Skavland J, Gavasso S, et al. Single cell immune profiling by mass cytometry of newly diagnosed chronic phase chronic myeloid leukemia treated with nilotinib. Haematologica. 2017;102(8):1361-1367. doi:10.3324/haematol.2017.167080
- Tislevoll BS, Hellesøy M, Fagerholt OHE, et al. Early response evaluation by single cell signaling profiling in acute myeloid leukemia. Nat Commun. 2023;14(1):115. Published 2023 Jan 7. doi:10.1038/s41467-022-35624-4