Zeiss LSM 510 META
Hovedinnhold
Room 6C112cA
Telephone 555 86581
This confocal system is reliable working horse and well equipped for a broad range of dyes, long term timelapse and emission fingerprinting (META detector). The most common samples are fixed cells and tissue and 4 seperate colors can easily be combined. A heating chamber covering the whole microscope and a CO2-controller makes long term timelapses feasible, cells have survived for more than a weekend under these conditions. The movie to the right shows cells happily dividing and moving around during a time period of 16 hours. The low magnification/NA objectives are of high quality making the miroscope well suited for also lager specimens, e.g. small fish larvae.
Responsible contact person is Hege Avsnes Dale.
Specifications
Equipped with 4 lasers, 2 regular detectors (PMTs) and a META detector, which may also serve as an additional detector. A transmission detector enables confocal "Pseudo-DIC". A heating chamber and a CO2 controller fasilitates live time experiments. In addition a CCD-camera is mounted for regular widefield fluorescence and brightfield imaging.
Lasers / Laser lines:
Blue diode (BD) - 25 mW / 405
Argon (Ar) - 30 mW / 458, 472, 488, 514
Helium/Neon I (HeNeI) - 1 mW / 543
Helium/Neon II (HeNeII) - 5 mW / 633
Objectives:
| M | NA | Type | IM | WD (mm) |
| 5x | 0.16 | Plan-Apochromat | Air | 12,2 |
| 10x | 0.30 | Plan-Neofluar | Air | 5,6 |
| 16x | 0.50 | Plan-Neofluar | Imm. | 0,7 |
| 20x | 0.50 | Plan-Neofluar | Air | 2,0 |
| 40x | 1.30 | Plan-Neofluar | Oil | 0,2 |
| 63x | 1.20 | C-Apochromat | Water | 0,24 |
| 63x | 1.40 | Plan-Apochromat | Oil | 0,19 |
| 100x | 1.45 | Alpha Plan-Fluar | Oil | 0,11 |
M: magnification
NA: numerical aperture
IM: immersion medium
WD: working distance
Links
LSM 5 Image Browser (Fill in the form and submit. Then you will be able to downlod the browser)
