Hjem
Molecular Imaging Center

Varselmelding

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Fast spinning precision

Andor Dragonfly 505 confocal

Hovedinnhold

Room 6C112cA

Responsible contact persons Endy Spriet and Hege Dale.

Dragonfly 505 (Andor Technologies, Inc) is a full featured high end confocal spinning disk system with a wide range of applications for cells and tissue. The system is equipped with an inverted Nikon Ti-E microscope stand including the Perfect Focus System (PFS) and a dual camera setup of iXon 888 Life EMCCD and Zyla 4.2 PLUS sCMOS. Available laser lines 405 (100 mW), 488 (150 mW), 561 (100 mW) and 637 nm (140 mW).

  • Ideal for any application that is time consuming on a regular scanning confocal, for example thick samples at lower magnification or large tile-sans.
  • Ideal for fast live cell imaging, with the ability to bleach and activate fluorescent proteins (Mosaic function).
  • TIRF (total internal reflection fluorescence) microscopy included.
  • SRRF-stream real time super resolution functionality.
  • dSTORM ready with 3D localization optics (reconstruction/visualization not implemented in the software).

For inspiration and application overview please read Andors "Dragonfly Twelve Reasons Why" under attachments.

Fusion software guide.

Objective

Magnification/NA

Immersion

WD (mm)

CFI Plan Apochromat Lambda

10x / 0.45

Air

4.0

CFI Plan Apochromat Lambda

20x / 0.75

Air

1.0

CFI Plan Apochromat Lambda S LWD

40x / 1.14

Water

0.59

CFI Plan Apochromat Lambda

60X / 1.40

Oil

0.14

CFI SR HP Apo TIRF

100x / 1.49

Oil

0.12

Field of view (FOV) with zoom optics (um)

Valid for both cameras

1x

1.5x

2x

10x

1234.9

823.3

617.4

20x

617.4

411.6

308.7

40x

308.7

205.8

154.4

60X

205.8

137.2

102.9

100x

123.5

82.3

61.7