Laboratory Methods
Below are some methods frequently used at PROBE. You are welcome to download these methods for use in your own laboratory.
Laboratory Methods Foto: Pål-Erik Rosenbaum
In Gel Digestion
Treatment:
1D and 2D SDS-PAGE spots, in-gel reduction and alkylation of cysteins, trypsin digestion, and peptide extraction and purification on nC18 RP-microcolumns.
Details: in-gel-proteindigestion.pdf
In Solution Digestion
Treatment:
Denaturation, reduction and alkylation of cysteins and trypsin digestion and purification on nC18 RP-microcolumns.
Details: In Solution Digestion
Last updated 17.1.2012
Download files
- In Solution Digestion (PDF-fil, 47 Kb)
- in-gel-proteindigestion.pdf (PDF-fil, 43 Kb)