Workshop on Single Molecule-based Super-resolution Microscopy: from Acquisition to Analysis, from Theory to Applications
Time and Venue:
21-23 September 2022, Bygg for Biologiske Basalfag (BBB, situated behind Haukeland Hospital), Jonas Lies vei 91, 5009 Bergen.
Hongyu ZHANG, Researcher, Department of Biomedicine, UiB
Department of Biomedicine, Molecular Imaging Center (MIC), Core facility for SMLM
L. Meltzers Høyskolefond
(TO NOTE, lectures are open to every one, without the need to register. So, you only need to register if you want to attend the hands-on sessions). We will recruit around 20 participants for hands-on sessions. Registration is FREE and will be open until 24th Aug; the applicants will be notified of registration status by 31th Aug. For registration, please follow the link:
Program: Download here.
Scope of teaching and/or hands on sessions:
1) Single particle tracking of endogenous proteins in the plasma membrane using Quantum Dots (Lecture + Hands-on session)
2) Single particle tracking of proteins in the nucleus by genetic fusion of Halo tag to the target protein and synthetic fluorescent ligands (Lecture + Hands-on session)
3) Single particle tracking of proteins by genetic fusion of a photoswitchable/photoactivatable protein to the target protein (Lecture + Hands-on session)
4) Single particle tracking of RNA in live cells (Lecture)
1. Robert H. SINGER, Member of National Academy of Sciences of USA, Member of American Academy of Arts & Sciences, Harold and Muriel Block Endowed Chair, Director of the Program in RNA Biology, Chairman Emeritus of Anatomy & Structural Biology, Co-Director of the Gruss Lipper Biophotonics Center, Professor of Cell Biology, Professor of Neuroscience, Albert Einstein College of Medicine, Group Leader at the Janelia Research Campus, USA.
Robert is a world-leading scientist focusing on the cell biology of RNA. He is a pioneer in studying single molecules of RNA in living cells and in devising methods to track RNAs through their life cycle. His studies have great implications in understanding the role of RNA in various disease processes, such as cancer metastasis and intellectual disability. His representative publications are: Nat Methods. 2018. 15(1):81-89; Science. 2016.17;352(6292):1430-5; Nat Rev Mol Cell Biol. 2015 Feb;16(2):95-109.
2. James Zhe LIU, group Leader at HHMI's Janelia Research Campus. He developed tools to study 3D genome architecture in the nucleus using live-cell 3D single-molecule localization by lattice light-sheet microscopy and 3D ATAC-PALM combined ATAC (Assay for Transposase-Accessible Chromatin) with lattice light-sheet PALM microscopy to selectively image and quantitatively analyze 3D accessible chromatin domains (ACDs) in single cells. His representative publications are: Nature Genetics 2022 (accepted); Nature Methods. 2020. 01;17(4):430-6; Cell. 2019. 30;177(6):1522-1535.e14; Science. 2015. 350(6262):823-6; Cell. 2015 162(4):705-7; Cell. 2014. 156:1274-85.
3. Christopher OBARA, a research associate in the Lippincott-Schwartz group at HHMI Janelia Research Campus. Jennifer Lippincott-Schwartz is a Member of National Academy of Sciences of USA. Lippincott-Schwartz has successfully utilized single-molecule based super-resolution imaging techniques to reveal an accurate picture of how the peripheral endoplasmic reticulum is structured, which provides new insights for genetic diseases affecting proteins that shape the endoplasmic reticulum. Moreover, Lippincott-Schwartz's lab has also demonstrated that Golgi enzymes constitutively recycle back to the endoplasmic reticulum and that such recycling plays a central role in the maintenance, biogenesis, and inheritance of the Golgi apparatus in mammalian cells.
Christopher OBARA is broadly interested in the role of physical forces as structuring components of cells, and his work is primarily in the structure and function of internal membranes in eukaryotes. He specializes in the correlation of advanced imaging technologies and quantitative approaches in cell biology. His most recent work has involved the development and implementation of a variety of tools to identify complex structures in the mammalian endoplasmic reticulum. Representative publications: Nature. 2022 Jan;601(7891):132-138, Nat Methods. 2021 Sep;18(9):1082-1090, Science. 2016 Oct 28;354(6311):aaf3928.
4. Hongyu ZHANG, Researcher at the Department of Biomedicine, UiB, organizer of this workshop. She is an expert on single-particle tracking of surface and intracellular proteins in live cells. She has successfully combined single particle tracking techniques with super-resolution imaging to identify the role of AMPA receptor trafficking in different disease models, such as Stress/depression and Huntington’s Disease. Her representative publications are : Nat Commun. 2018,15;9(1):4272; J Clin Invest 2016, 126(2)627-63; Mol Psychiatry. 2013 Apr;18(4):471-84.
5. Dominik Frei (Inter Instruments AS, Oslo, Norway)
6. Hege Dale (Molecular Imaging Center, UiB)