Optical Imaging.

Room 3030, 3rd floor Vivarium, HUS

Traditional in vivo optical molecular imaging systems grossly measure all of the photons that propagate through tissue without any temporal discrimination. This is known as the Continuous Wave (CW) technique. The method is limited to providing the attenuation or the total loss of photons in tissue. It cannot discriminate absorption events from scattering events, which impedes its capability to uncouple location (depth) from concentration in the image.

Bioluminescence measurements by definition are CW since they are not generated in response to a light stimulus. In Time Domain (TD) optical imaging, short pulses of light are sent to illuminate the specimen under study. The system then detects the photons according to their time-of-flight within the tissue. This time-of-flight distribution (generally called a TPSF or Temporal Point Spread Function) is used to recover the optical characteristics of the specimen, discriminating absorption from scattering properties. In addition to enabling the uncoupling of depth and concentration, scattering gives additional information for imaging disease and physiological processes.

Responsible contact person is Emmet Mc Cormack.

• Specifications

Supplier/Model:

• IVIS Spectrum In Vivo Imaging System (PerkinElmer)
• Optix MX3 Molecular Imaging System, pulsed laser (ART)

Applications:

       In vivo detection of near IR and GFP/Luminescent probe

• Links

IVIS Spectrum