Nils Åge Frøystein's picture

Nils Åge Frøystein

Associate Professor
  • E-mailNils.Froystein@uib.no
  • Phone+47 55 58 35 46
  • Visitor Address
    Realfagbygget, Allégt. 41
  • Postal Address
    Postboks 7803
    5020 BERGEN

My research is mostly concerned with the application of high-resolution multinuclear (1H, 13C, 15N) og multidimensional (2D/3D) NMR-spectroscopy of isotopically enriched (13C, 15N) proteins in solution and 2D NMR spectroscopy of proteinbinding peptides. The NMR spectra can give us information about structure of molecules, dynamical processes (exchange) and interactions between for instance larger biomolecules and smaller ligand molecules. I am mainly interested in the methodological aspects of NMR spectroscopy, and I am working with NMR spectroscopy applied to many areas within chemistry.


I am currently teaching the following courses:

KJEM100 / Chemistry in nature

KJEM306 / NMR Spectroscopy II

Master projects

NMR spectroscopy of proteins and peptides: Master projects are offered within the application of high resolution multinuclear (1H, 13C, 15N and others) and multidimensional (1D, 2D and 3D) NMR spectroscopy applied to proteins, peptides and smaller molecules that are interacting with proteins. NMR spectroscopy can give us important information about the function and working mechanisms of the protein at the molecular level in solution. This include structural information about the active sites, the binding of peptides and smaller molelcules (such as substrates, cofactors, inhibitors and drug molecules) and dynamical processes (the exchange of smaller ligand molecules). The choise of NMR-methods and post-processing procedures will depend strongly on the kind of information sought in each case.

This research is a cooperation with the department of Molecular Biology.

Academic article
  • Drageset, Audun; Frøystein, Nils Åge; Törnroos, Karl Wilhelm; Bjørsvik, Hans-Rene. 2019. A two-step telescoped continuous flow switchable process leading to nitriles, diaziridine or hydrazine derivatives. Reaction Chemistry & Engineering. 41-51.
  • Quadri, Coralie Celine; Ralte, Lalrempuia; Frøystein, Nils Åge; Törnroos, Karl Wilhelm; Le Roux, Erwan. 2018. Steric factors on unsymmetrical O-hydroxyaryl N-Heterocyclic carbene ligands prevailing the stabilization of single stereoisomer of bis-ligated titanium complexes. Journal of Organometallic Chemistry. 106-116.
  • Hahn, Friedrich; Setz, Christian; Friedrich, Melanie; Rauch, Pia; Solbak, Sara Marie Øie; Frøystein, Nils Åge; Henklein, Petra; Votteler, Jörg; Fossen, Torgils; Schubert, Ulrich. 2014. Mutation of the highly conserved ser-40 of the HIV-1 p6 gag protein to phe causes the formation of a hydrophobic patch, enhances membrane association, and polyubiquitination of gag. Viruses. 3738-3765.
  • Skår, Hjørdis; Seland, John Georg; Liang, Yucang; Frøystein, Nils Åge; Törnroos, Karl Wilhelm; Anwander, Reiner. 2013. Screening of the relaxivity of gadolinium-loaded periodic mesoporous silica functionalized by means of soft metalorganic silylamide grafting. European Journal of Inorganic Chemistry. 5969-5979.
  • Brenner, Annette; Kieffer, Bruno; Trave, Gilles Michel; Frøystein, Nils Åge; Raae, Arnt Johan. 2012. Thermal stability of chicken brain alpha-spectrin repeat 17: a spectroscopic study. Journal of Biomolecular NMR. 71-83.
  • Evjenth, Rune Henrik; Brenner, Annette; Thompson, Paul R.; Arnesen, Thomas; Frøystein, Nils Åge; Lillehaug, Johan R. 2012. Human Protein N-terminal Acetyltransferase hNaa50p (hNAT5/hSAN) Follows Ordered Sequential Catalytic Mechanism (combined kinetic and NMR study). Journal of Biological Chemistry. 10081-10088.
  • Occhipinti, Giovanni; Jensen, Vidar Remi; Törnroos, Karl Wilhelm; Frøystein, Nils Åge; Bjørsvik, Hans-Rene. 2009. Synthesis of a new bidentate NHC–Ag(I) complex and its unanticipated reaction with the Hoveyda–Grubbs first generation catalyst. Tetrahedron. 7186-7194.
  • Alvheim, Cecilie; Frøystein, Nils Åge; Vinje, Jo; Intini, Francesco P.; Natile, Giovanni; Liu, Yangzhong; Huang, Rongong; Sletten, Einar. 2009. Cytotoxic trans-oriented iminoether platinum complexes - Kinetics of binding to DNA oligonucleotides determined by 15-N NMR spectroscopy. Inorganica Chimica Acta. 907-914.
  • Sommerfeldt, Hanne-Marthe; Meerman, Christian; Schrems, Marcus; Törnroos, Karl Wilhelm; Frøystein, Nils Åge; Miller, Robert J.; Scheidt, Ernst-Wilhelm; Scherer, Wolfgang; Anwander, Reiner. 2008. Characterization and reactivity of peralkylated Ln(II)Al(III) heterobimetallic complexes. Dalton Transactions. 1899-1907.
  • Zimmermann, Melanie; Frøystein, Nils Åge; Fischbach, Andreas; Sirsch, Peter; Dietrich, Hans Martin; Törnroos, Karl Wilhelm; Herdtweck, Eberhardt ; Anwander, Reiner. 2007. Homoleptic rare-earth metal(III) tetramethylaluminates: Structural chemistry, reactivity, and performance in isoprene polymerization. Chemistry - A European Journal. 8784-8800.
  • Grove, Hilde; Frøystein, Nils Åge; Sæthre, Leif J; Sletten, Jorunn. 2006. Crystalline products isolated from solutions with commercially available 2,3-bis(2-pyridyl)pyrazine (dpp) as reactant: Detection of a dimerized form of dpp. Journal of Molecular Structure. 1-17.
  • Halskau, Øyvind; Underhaug, Jarl; Frøystein, Nils Åge; Martinez, Aurora. 2005. Conformational flexibility of alpha-lactalbumin related to its membrane binding capacity. Journal of Molecular Biology. 1072-1086.
  • Teigen, Knut; Dao, Khanh Kim; Mckinney, Jeffrey Alan; Gorren, A.C.F.; Mayer, B.; Frøystein, Nils Åge; Haavik, Jan; Martinez, Aurora. 2004. Tetrahydrobiopterin binding to aromatic amino acid hydroxylases. Ligand recognition and specificity. Journal of Medicinal Chemistry. 5962-5971.
  • Doudin, K.; Berge, Rolf Kristian; Frøystein, Nils Åge; Songstad, Jon. 2000. Preparation and NMR studies of 3-selenaheptadecanoic and 4-selenaoctadecanoic acid, RSe(CH2)nC(O)OH (R = -C14H29, n = 1 and 2), and their methyl esters. Journal of the Chemical Society. Perkin Transactions 2 (2001). 723-729.
  • Teigen, Knut; Frøystein, Nils Åge; Martinez, Aurora. 1999. The structural basis of the recognition of phenylalanine and pterin cofactors by phenylalanine hydroxylase. Implications for the catalytic mechanism. Journal of Molecular Biology. 807-823.
  • Teigen, Knut; Frøystein, Nils Åge; Martinez, Aurora. 1999. The structural Basis of the Recognition of Phenylalanine and Pterine Cofactors by Phenylalanine Hydroxylase. Implications for the Catalytic Mechanism. Journal of Molecular Biology. 807-823.
  • Fossen, Torgils; Frøystein, Nils Åge; Andersen, Øyvind Moksheim. 1998. Myricetin 3-rhamnopyranosyl (1-6) galactoside from Nymphaea x marliacea. Phytochemistry. 1997-2000.
  • Moldrheim, Erlend; Andersen, Bjørn; Frøystein, Nils Åge; Sletten, Einar. 1998. Interaction of Manganese (II)Cobalt(II) and Nickel(I) with DNA Oligomers Studied by Proton NMR Spectroscopy. Inorganica Chimica Acta. 41-46.
  • Garoufis, A.; Perlepes, S.P.; Frøystein, Nils Åge; Sletten, Jorunn; Hadjiliadis, J. 1996. Mercuration of acetone in the acetone-mercury(II) perchlorate2-('2-pyridyl)quinoxaline(L) system. Polyhedron. 1035-1039.
  • Frøystein, Nils Åge; Sletten, Einar. 1994. The binding of mercury(II) to the syntetic oligonucleotide d(CGcGAATTCGCG)%cA %1H adn %1%5N NMR study. Journal of the American Chemical Society. 3240-3250.
  • Frøystein, Nils Åge; Davis, J. T.; Reid, B. R.; Sletten, Einar. 1993. Sequence-Selective Metal Ion Binding to DNA Oligonucleotides. Acta Chemica Scandinavica. 649-657.
  • Steinkopf, Signe; Frøystein, Nils Åge; Liu, Q.; Sletten, Einar. 1992. Copper(II) - Purine Complexes Studied by Proton and Nitrogen-15 Nuclear Magnetic Resonance. Acta Chemica Scandinavica. 446-450.
  • Stavropoulos, George; Karagiannis, Kostas; Vynios, Dimitrios; Papaioannou, Dionissios; Aksnes, Dagfinn; Frøystein, Nils Åge; Francis, George W. 1991. Solid-Phase Synthesis and Spectroscopic Studies of TRH Analogues Incorporating Cis- and trans-4-Hydroxy-L-Proline. Acta Chemica Scandinavica. 1047-1054.
Academic lecture
  • Sletten, Einar; Frøystein, Nils Åge. 2008. NMR kinetic studies using isotope labeled reagents.
  • Teigen, Knut; Frøystein, Nils Åge; Martínez, Aurora. 1998. NMR study on the conformation of L-phenylalanine and dihydrobiopterin bound to wild-type and mutant forms of recombinant human phenylalanine hydroxylase.
Short communication
  • Brenner, Annette; Frøystein, Nils Åge. 2014. Using MUSIC and CC(CO)NH for Backbone Assignment of Two Medium-Sized Proteins Not Fully Accessible to Standard 3D NMR. Molecules. 8890-8903.
Thesis at a second degree level
  • Underhaug, Jarl. 2004. An NMR study of alpha-lactalbumin: pKa-determinations and structural calculations.
Masters thesis
  • Lien, Lars Elstad. 2015. PELARGONIDIN-3-GLUCOSIDE: AN APPROACH TO STRUCTURE ELUCIDATION BY NMR - Choosing the right experiments.
Doctoral dissertation
  • Frøystein, Nils Åge. 1993. Sequence-selective interactions of DNA and metal ions : NMR studies of the interaction of divalent transition metal ions with oligodeoxyribonucleotides.
Academic chapter/article/Conference paper
  • Sletten, Einar; Frøystein, Nils Åge. 2009. Sequence-Selective Binding of Transition Metal Complexes to DNA. 28 pages.
  • Sletten, Einar; Frøystein, Nils Åge. 1996. NMR studies of oligonucleotide - metal ion complexes. 1 pages.
  • Sletten, Einar; Frøystein, Nils Åge. 1996. NMR Studies of Oligonucleotide-Metal Ion Interactions. 19 pages.
  • Sletten, Einar; Frøystein, Nils Åge. 1995. NMR spectroscopic structure determination of metal ion - oligonucleotide complexes - the results of sequence-selective binding studies. 8 pages.
  • Zimmermann, Melanie; Frøystein, Nils Åge; Törnroos, Karl Wilhelm; Anwander, Reiner. 2006. Dissociative versus Associative Alkyl Exchange: An Extended Dynamic NMR Spectroscopic Study of Ln(AlMe4)3.

More information in national current research information system (CRIStin)

Research groups