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Norwegian Multiple Sclerosis Competence Centre and Research Group
In-depth characterization of the cerebrospinal fluid (CSF) proteome

New study: In-depth characterization of the cerebrospinal fluid (CSF) proteome displayed through the CSF proteome resource (CSF-PR).

Researchers at the KGJ Centre for MS Research have published a paper in Mol Cell Proteomics mapping the human cerebrospinal fluid (CSF) proteome and the results are freely available via the new CSF Proteome Resource (http://probe.uib.no/csf-pr).

In this study, the human cerebrospinal fluid (CSF) proteome was mapped using three different strategies prior to Orbitrap LC-MS/MS analysis: SDS-PAGE and mixed-mode reversed phase anion exchange for mapping the global CSF proteome, and hydrazide-based glycopeptide capture for mapping glycopeptides. A maximal protein set of 3081 proteins (28,811 peptide sequences) was identified, of which 520 were identified as glycoproteins from the glycopeptide enrichment strategy, including 1121 glycopeptides and their glycosylation sites. To our knowledge this is the largest number of identified proteins and glycopeptides reported for CSF, including 417 glycosylation sites not previously reported. From parallel plasma samples, we identified 1050 proteins (9739 peptide sequences). An overlap of 877 proteins was found between the two body fluids, while 2204 proteins were identified only in CSF and 173 only in plasma. All mapping results are freely available via the new CSF Proteome Resource (http://probe.uib.no/csf-pr), which can be used to navigate the CSF proteome and help guide the selection of signature peptides in targeted quantitative proteomics.

 

Read more about the method at KGJ Centre for MS Research.

 

Pubmed:

Guldbrandsen A1, Vethe H2, Farag Y2, Oveland E1, Garberg H1, Berle M3, Myhr KM3, Opsahl JA1, Barsnes H1, Berven FS4.In-depth characterization of the cerebrospinal fluid proteome displayed through the CSF Proteome Resource (CSF-PR).Mol Cell Proteomics. 2014 Jul 18. pii: mcp.M114.038554. [Epub ahead of print]