Below are some methods frequently used at PROBE. You are welcome to download these methods for use in your own laboratory.
In Gel Digestion
1D and 2D SDS-PAGE spots, in-gel reduction and alkylation of cysteins, trypsin digestion, and peptide extraction and purification on nC18 RP-microcolumns.
Details: In-Gel Digestion
In Solution Digestion
Denaturation, reduction and alkylation of cysteins and trypsin digestion and purification on nC18 RP-microcolumns.
Details: In Solution Digestion