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Laboratory Methods

Below are some methods frequently used at PROBE. You are welcome to download these methods for use in your own laboratory.

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In Gel Digestion

Treatment:

1D and 2D SDS-PAGE spots, in-gel reduction and alkylation of cysteins, trypsin digestion, and peptide extraction and purification on nC18 RP-microcolumns.

Details: In-Gel Digestion

In Solution Digestion, 8M Urea

Treatment:

Denaturation, reduction and alkylation of cysteins and trypsin digestion and purification on nC18 RP-microcolumns.

Details: In Solution Digestion

In Solution Digestion, 6M GndHCl

Treatment

Under contruction

In Solution Digestion, SP3

Treatment

Under contruction

In Solution Digestion, FASP

Treatment

Under contruction