Recombinant enzyme/protein expression, purification & characterization November 26-30 2018
The course aims to give an introduction and basic skills in recombinant protein/enzyme production, purification and characterization. The course participants will be guided in the planning of expression and purification projects, primer design for PCR cloning, and protein expression in E.coli. The course will focus upon optimization of the various steps in the experimental procedures and focus upon trouble shooting.
Main content
Dates: 26-30.11 2018
Place: UiB
Course responsible: Øyvind Halskau
Exam: Laboratory report
Credits: 3 ECTS
Registration: Deadline 26.10.2018, register here: https://skjemaker.app.uib.no/view.php?id=5416332
The course is organized in cooperation with the BioCat national research school. Course organizers and tutors are
- Gunter Stier, Heidelberg University Biochemistry Centre, Heidelberg, Germany
- Gilles Travé, Institute de génétique et de biologie moléculaire et cellulaire (IGMBC), Strasbourg, France
External participants
Participants affiliated with other institutions than the University of Bergen, can have their credits formally registered as guest students if:
You are formally admitted to the PhD programme at another institution
- You have a written confirmation/admission letter showing that the course is going to be part of the course plan ("opplæringsdelen") of your PhD.
- You have a Norwegian ID-number ("personnummer"/"D-nummer").
If so, we will need your Norwegian ID-number, your full name (as written in your passport/ID) and citizenship. Please contact Knut Olav Daasvatn (tlf. 55 58 45 03) to provide the information needed.
Course description
The course aims to give an introduction and basic skills in recombinant protein/enzyme production, purification and characterization.
The course participants will be guided in the planning of expression and purification projects, primer design for PCR cloning, and protein expression in E.coli. The course will focus upon optimization of the various steps in the experimental procedures and focus upon trouble shooting.
The candidate will, after completing the course, have:
Knowledge within
- Primer design and gene optimization
- Insights in various trouble shooting problems and strategies to solve them
- Insight in medium throughput cloning and protein expression in E. coli
- Simple application of NMR to assess protein fold after purification
- Co-expression and purification strategies
- Insight in mutagenesis techniques
Skills in
- Cloning of genes into vectors containing different tags and fusion proteins
- Use of bioinformatics in analysis and planning of a protein expression project
- Protein expression and mini screen of the expression
- Assess different E.coli strains, growth conditions and fusion partners to optimize the quality of expressed protein
- Simple enzymatic activity assays
General competence in
- Rational project planning
- Use of fusion protein- and alternative purification strategies
- Scale-up of positive candidates to a protein midi-preparation using purification machines
Practical information
The course is held at Høyteknologisenteret i Bergen (HIB), Thormølensgate 55. The lab and lecture room is in the 4th floor of the Biobuilding ("Bioblokken"):