Automated Sanger DNA Sequencing is performed using capillary-based Applied Biosystem 3730XL Analyzer. The instrument offers high throughput and sensitivity. Samples of single stranded, double stranded DNA, and PCR products, can be sequenced with reads >800 base pairs.
The sequencing PCR is performed by the user with high-quality template DNA, 1 primer, BigDye terminator 3.1 mix and sequencing buffer.
The clean-up of DNA at the sequencing lab is robotized.
Sequences are usually available overnight on our server.