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Svein Are Mjøs's picture

Svein Are Mjøs

Associate Professor
  • E-mailSvein.Mjos@uib.no
  • Phone+47 55 58 35 53
  • Visitor Address
    Realfagbygget, Allégt. 41
    Room 
    2016
  • Postal Address
    Postboks 7803
    5020 BERGEN

Data treatment for analytical raw data 

 

In instrumental analysis like chromatography or chromatography coupled to mass spectrometry, algorithms for compound identification and quantification are usually applied more or less directly on the raw data. However, much can be done to improve the quality of the data prior to identification and quantification. Development and application of methodologies for noise reduction, deconvolution of overlapping signals, and standardization of spectra are important research activities in this field.

Compound identification based on multivariate methodology

In many cases statistical methods will reveal more information about compound structure than classical interpretation of spectra. In chromatographic analyses the proper use of retention indices may contribute significantly to compound elucidation. Research in this field include interpretation of mass spectra by pattern recognition and regression, modelling of retention indices and combined use of spectral and retention information for identification purposes.

Optimization and validation of analytical methods

Research activities in this area include the use of response surface methodology for optimization of chromatographic resolution, the systematic treatment of chromatographic retention, and studies on the efficiency of extraction methods.

Analytical applications

Methodologies described above are applied on analysis of lipids, organic pollutants and volatile organic compounds. Applied techniques are gas chromatography (GC), liquid chromatography (LC), mass spectrometry (MS), and hyphenated techniques like GC-MS and LC-MS.

 

Teaching

I am currently teaching the course KJEM/FARM-250 Analytical Chemistry.

Master projects

Master projects are offered within analytical chemistry and chemometrics and within environmental chemistry.

Projects in analytical chemistry will usually be focused on analysis of lipids using gas chromatography, liquid chromatography or mass spectrometry. Characterization of lipids is important in fields like medicine, nutrition and studies of environmental effects. Data handling by chemometric techniques will be essential in most projects.

Projects in environmental chemistry will be focused on analysis of abundance, effects or properties of organic pollutants using chromatographic and mass spectrometric methodology. Master projects are usually carried out in close cooperation with other research groups in Bergen.

 

 

Academic article
  • 2020. Minor compounds and potential interferents in gas chromatographic analyses of human serum fatty acids. Journal of chromatography. B. 10 pages.
  • 2020. Effects of intact and hydrolysed blue whiting proteins on blood pressure and markers of kidney function in obese Zucker fa/fa rats. European Journal of Nutrition. 16 pages.
  • 2019. Effects of low doses of fish and milk proteins on glucose regulation and markers of insulin sensitivity in overweight adults: a randomised, double blind study. European Journal of Nutrition. 1-17.
  • 2018. Water-soluble fish protein intake led to lower serum and liver cholesterol concentrations in obese zucker fa/fa rats. Marine Drugs. 1-12.
  • 2018. Salmon fillet intake led to higher serum triacylglycerol in obese zucker Fa/Fa rats but not in normolipidemic Long-Evans rats. Nutrients. 1-13.
  • 2018. Intake of baked cod fillet resulted in lower serum cholesterol and higher long chain n-3 PUFA concentrations in serum and tissues in hypercholesterolemic obese Zucker fa/fa rats. Nutrients. 1-15.
  • 2018. Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors. Frontiers in Plant Science. 1-11.
  • 2018. Comparing EPA production and fatty acid profiles of three Phaeodactylum tricornutum strains under western Norwegian climate conditions. Algal Research. 11-22.
  • 2018. Chromatographic efficiency of polar capillary columns applied for the analysis of fatty acid methyl esters by gas chromatography. Journal of Separation Science. 1582-1592.
  • 2017. Lipids of Dietzia sp. A14101. Part II: A study of the dynamics of the release of surface active compounds by Dietzia sp. A14101 into the medium. Chemistry and Physics of Lipids. 31-42.
  • 2017. Lipids of Dietzia sp. A14101. Part I: A study of the production dynamics of surface-active compounds. Chemistry and Physics of Lipids. 19-30.
  • 2017. High intake of fatty fish, but not of lean fish, improved postprandial glucose regulation and increased the n-3 PUFA content in the leucocyte membrane in healthy overweight adults: A randomised trial. British Journal of Nutrition. 1368-1378.
  • 2017. Effects of baked and raw salmon fillet on lipids and n-3 PUFAs in serum and tissues in Zucker fa/fa rats. Food & Nutrition Research. 1-12.
  • 2017. Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies. Algal Research. 392-401.
  • 2016. Specific metabolites in a Phaeodactylum tricornutum strain isolated from Western Norwegian fjord water. Marine Drugs. 17 pages.
  • 2016. Serum fatty acid and lipoprotein subclass concentrations and their associations in prepubertal healthy Norwegian children. Metabolomics.
  • 2016. Predictive associations between serum fatty acids and lipoproteins in healthy non-obese Norwegians: implications for cardiovascular health. Metabolomics.
  • 2016. Plackett-Burman design and fragmentation studies to assist the 1 comparison of techniques used to extract phospholipids prior to regiospecific characterization by liquid chromatography mass spectrometry. American Journal of Modern Chromatography. 1-22.
  • 2016. Methylmercury Increases and Eicosapentaenoic Acid Decreases the Relative Amounts of Arachidonic Acid-Containing Phospholipids in Mouse Brain. Lipids. 61-73.
  • 2016. Intensive lifestyle intervention provides rapid reduction of serum fatty acid levels in women with severe obesity without lowering omega-3 to unhealthy levels. Clinical Obesity. 259-267.
  • 2016. Extension of least squares spectral resolution algorithm to high-resolution lipidomics data. Analytica Chimica Acta. 35-46.
  • 2016. Dietary fish protein hydrolysates containing bioactive motifs affect serum and adipose tissue fatty acid compositions, serum lipids, postprandial glucose regulation and growth in obese Zucker fa/fa rats. British Journal of Nutrition. 1336-1345.
  • 2016. Changes in serum fatty acid and lipoprotein subclass concentrations from prepuberty to adulthood and during aging. Metabolomics. 10 pages.
  • 2016. Application of gas chromatography/tandem mass spectrometry to determine a wide range of petrogenic alkylated polycyclic aromatic hydrocarbons in biotic samples. Rapid Communications in Mass Spectrometry. 2052-2058.
  • 2016. An LC–MS-based lipidomics approach for studying the impact of dietary eicosapentaenoic acid on modulating methylmercury toxicity in mice. Metabolomics.
  • 2015. Variable Number of Tandem Repeats (VNTR) analysis of Flavobacterium psychrophilum from salmonids in Chile and Norway. BMC Veterinary Research.
  • 2015. Optimizing the relationship between chromatographic efficiency and retention times in temperature-programmed gas chromatography. Journal of Separation Science. 3014-3027.
  • 2015. Metabolic markers in blood can separate prostate cancer from benign prostatic hyperplasia. British Journal of Cancer. 1712-1719.
  • 2015. Fatty acids in bacterium Dietzia sp grown on simple and complex hydrocarbons determined as FAME by GC-MS. Chemistry and Physics of Lipids. 15-26.
  • 2015. Data on pigments and long-chain fatty compounds identified in Dietzia sp. A14101 grown on simple and complex hydrocarbons. Data in Brief. 622-629.
  • 2015. Changes in Serum Fatty Acid Levels During the First Year After Bariatric Surgery. Obesity Surgery.
  • 2014. Transfer of retention patterns in gas chromatography by means of response surface methodology. Journal of Chromatography A. 64-72.
  • 2014. Influence of type of raw material on fishmeal physicochemical properties, the extrusion process, starch gelatinization and physical quality of fish feed. Aquaculture Nutrition. 410-420.
  • 2014. Evaluation of the retention pattern on ionic liquid columns for gas chromatographic analyses of fatty acid methyl esters. Journal of Chromatography A. 83-91.
  • 2014. Effects of duodenal switch alone or in combination with sleeve gastrectomy on body weight and lipid metabolism in rats. Nutrition and Diabetes. 6 pages.
  • 2014. Determination of Omega-3 Fatty Acids in Fish Oil Supplements Using Vibrational Spectroscopy and Chemometric Methods . Applied Spectroscopy. 1190-1200.
  • 2014. A low dietary intake of cod protein is sufficient to increase growth, improve serum and tissue fatty acid compositions, and lower serum postprandial glucose and fasting non-esterified fatty acid concentrations in obese Zucker fa/fa rats. European Journal of Nutrition. 1151-1160.
  • 2013. Least squares spectral resolution of liquid chromatography-mass spectrometry data of glycerophospholipids. Journal of Chromatography A. 23-34.
  • 2013. Krill hydrolysate free amino acids responsible for feed intake stimulation in Atlantic salmon (Salmo salar). Aquaculture Nutrition. 47-61.
  • 2013. Impact of variability in fishmeal physicochemical properties on the extrusion process, starch gelatinization and pellet durability and hardness. Animal Feed Science and Technology. 77-84.
  • 2013. A database of chromatographic properties and mass spectra of fatty acid methyl esters from omega-3 products. Journal of Chromatography A. 94-102.
  • 2012. Trans isomers of EPA and DHA in Omega-3 products on the European market. Lipids. 659-667.
  • 2012. High growth rates in Atlantic salmon (Salmo salar L.) fed 7.5% fish meal in the diet. Micro-, ultra- and nano-filtration of stickwater and effects of different fractions and compounds on pellet quality and fish performance. Aquaculture. 134-146.
  • 2012. Efficiencies of three common lipid extraction methods evaluated by calculating mass balances of the fatty acids. Journal of Food Composition and Analysis. 198-207.
  • 2011. Trans Fatty Acid Analyses in Samples of Marine Origin: The Risk of False Positives. Journal of Agricultural and Food Chemistry. 3520-3531.
  • 2011. Experimental designs for modeling retention patterns and separation efficiency in analysis of fatty acid methyl esters by gas chromatography-mass spectrometry. Journal of Chromatography A. 6823-6831.
  • 2010. The vector of calibration ratios: A simple transfer method for mass spectra. Journal of Chromatography A. 5986-5994.
  • 2009. A Rank annihilation approach to reducing the scan-effect in gas chromatography-mass spectrometry data. Analytica Chimica Acta. 33-39.
  • 2008. Retention behavior of trans isomers of eicosapentaenoic and docosahexaenoic acid methyl esters on a polyethylene glycol stationary phase. European Journal of Lipid Science and Technology. 547-553.
  • 2007. Identification of estrogen-like alkylphenols in produced water from offshore oil installations. Marine Environmental Research. 651-655.
  • 2006. Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues. Journal of Chromatography A. 291-298.
  • 2006. Prediction of equivalent chain lengths from two-dimensional fatty acid retention indices. Journal of Chromatography A. 249-254.
  • 2006. Patterns in volatile components over heated fish powders. Food Research International. 190-202.
  • 2006. Geometrical isomerisation of double bonds in acid catalysed preparation of fatty acid methyl esters. European Journal of Lipid Science and Technology. 315-322.
  • 2006. Geometrical isomerisation of EPA and DHA at high temperatures. European Journal of Lipid Science and Technology. 589-597.
  • 2006. Alkylphenol retention indices. Journal of Chromatography A. 98-105.
  • 2005. Properties of trans isomers of eicosapentaenoic acid and eicosahexaenoic acid methyl esters on cyanopropyl stationary phases. Journal of Chromatography A. 185-192.
  • 2004. Two-dimensional fatty acid retention indices. Journal of Chromatography A. 201-209.
  • 2004. The prediction of fatty acid structure from selected ions in electron impact mass spectra of fatty acid methyl esters. European Journal of Lipid Science and Technology. 550-560.
  • 2004. Quantification of linolenic acid isomers by gas chromatography-mass spectrometry and deconvolution of overlapping chromatographic peaks. European Journal of Lipid Science and Technology. 307-318.
  • 2003. Spectral transformations for deconvolution methods applied on gas chromatography–mass spectrometry data. Analytica Chimica Acta. 231-241.
  • 2003. Identification of fatty acids in gas chromatography by application of different temperature and pressure programs on a single capillary column. Journal of Chromatography A. 151-161.
  • 2003. Determination of trans double bonds in polyunsaturated fatty acid methyl esters from their electron impact mass spectra. European Journal of Lipid Science and Technology. 156-164.
  • 2003. A rapid method for the analysis of hydrogenated fats by GC with IR detection. Journal of the American Oil Chemists Society. 839-846.
Report
  • 2011. Rapport/Report 21/2011 English summary. 21/2011. 21/2011. .
  • 2007. Rapport/Report 26/2007 English summary. 26/2007. 26/2007. .
Academic lecture
  • 2019. The response of a boreal deep-sea sponge holobiont to an acute crude oil exposure: a mesocosm experiment.
  • 2018. Fishmeal for FEED – Physical quality effects.
  • 2018. Effects of production method on technical properties of fishmeal intended for feed production.
  • 2017. Application of gas chromatography/tandem mass spectrometry to determine a wide range of petrogenic polycyclic aromatic hydrocarbons in small samples of oil exposed fish egg.
  • 2016. Bioprospecting for high PUFA content in Northern marine microalgae.
  • 2012. TRANSFER OF RETENTION PATTERNS IN GAS CHROMATOGRAPHY USING RESPONSE SURFACE METHODOLOGY.
  • 2012. En introduksjon til kjemometri.
  • 2012. Effects of a high fish intake on leukocyte membrane fatty acid composition and leukocyte function ex vivo in overweight and obese adults.
  • 2012. APPLICATION OF CHEMOMETRIC AND COMPUTATIONAL TOOLS FOR THE ANALYSIS OF MOLECULAR LIPID SPECIES BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY.
  • 2011. Multivariate modelling of extruded fish feed pellet quality.
  • 2004. The use of spectral information for quantification of overlapping chromatographic peaks. Some examples from GC-MS analyses of fatty acid methyl esters.
Thesis at a second degree level
  • 1996. Effekter på fettsyresammensetningen i egg fra atlantisk torsk (Gadus morhua) : bruk av gasskromatografi og multivariate metoder.
Doctoral dissertation
  • 2018. High-value fatty acids from microalgae. Bioprospecting and outdoor cultivation at northern latitudes.
  • 2015. Fishmeal physicochemical properties – Impact on the fish feed extrusion process, phase transitions and physical pellet quality.
  • 2015. Application of liquid chromatographymass spectrometry and chemometrics in the automated characterization of molecular lipid species.
  • 2006. Interpretation of chromatographic and mass spectrometric data from analyses of fatty acid methyl esters – Application of multivariate methods.
Abstract
  • 2014. Effect of two types of bariatric surgery on the fatty acid profile in serum. The Complexity of obesity proceedings.
Poster
  • 2017. Different outdoor performance of three Phaeodactylum tricornutum strains under western Norwegian climate conditions.
  • 2017. Bioprospecting North Atlantic microalgae with fast growth and high EPA/DHA content.
  • 2016. Effects of intake of baked and raw fish on serum lipids and fatty acid composition in obese rats.
  • 2016. Application of multi-dimensional gas chromatography mass spectrometry techniques to explain the increased sensitivity of Atlantic haddock embryos to crude oil pollution.
  • 2015. Stability of phospholipid analytical standards during storage.
  • 2015. Screening concept to prospect Nordic microalgae for polyunsaturated fatty acids.
  • 2015. INTAKE OF FISH PROTEIN AND EFFECTS ON GLUCOSE REGULATION AND LIPID METABOLISM IN OBESE ZUCKER RATS.
  • 2010. Choice of experimental design for modeling retention patterns and separation efficiency in GC/MS analysis of fatty acid methyl esters.

More information in national current research information system (CRIStin)

L. Zhang, S.A. Mjøs, S. Meier, O.M. Kvalheim, Y. Liang (2010) The vector of calibration ratios: A simple transfer method for mass spectra. J. Chromatogr. 1217: 5986-5994 [DOI: 10.1016/j.chroma.2010.07.039]

S.A. Mjøs, B. Grung (2009) A rank annihilation approach to reducing the scan-effect in gas chromatography – mass spectrometry data. Anal. Chim. Acta 640: 33-39 [DOI: 10.1016/j.aca.2009.03.011]

S.A. Mjøs (2008) Trans isomers of eicosapentaenoic and docosahexaenoic methyl esters on a polyethylene glycol (PEG) stationary phase. Eur. J. Lipid Sci. Technol. 110: 547-553 [DOI: 10.1002/ejlt.200700225]

S. Boitsov, S.A. Mjøs, S. Meier (2007) Identification of estrogen-like alkylphenols in produced water from offshore oil installations. Marine Environmental Research 64: 651-665 [DOI: 10.1016/j.marenvres.2007.07.001]

S.A. Mjøs (2006) Interpretation of chromatographic and mass spectrometric data from analyses of fatty acid methyl esters – Application of multivariate methods, PhD thesis, University of Bergen [ISSN: 82-308-0260-2]

S.A. Mjøs, M. Solvang (2006) Geometrical isomerisation of EPA and DHA at high temperatures. Eur. J. Lipid Sci. Technol. 108: 589-597 [DOI: 10.1002/ejlt.200600055]

S.A. Mjøs (2006) Prediction of equivalent chain lengths from two-dimensional fatty acid retention indices. J. Chromatography A. 1122: 249-254 [DOI: 10.1016/j.chroma.2006.04.067]

S.A. Mjøs, S. Boitsov, S. Meier (2006) Alkylphenol retention indices (APRI). J. Chromatography A. 1123: 98-105 [DOI: 10.1016/j.chroma.2006.05.002].

S.A. Mjøs, O. Grahl-Nielsen (2006) Prediction of gas chromatographic retention of polyunsaturated fatty acid methyl esters. J. Chromatography A. 1110: 171-180 [DOI: 10.1016/j.chroma.2006.01.092]

S.A. Mjøs, S. Meier, O. Grahl-Nielsen, Geometrical isomerisation of double bonds in acid catalysed preparation of fatty acid methyl esters. Eur. J. Lipid Sci. Technol. 108: 315-322 [DOI: 10.1002/ejlt.200500298]

S. Meier, S.A. Mjøs, H. Joenssen, O. Grahl-Nielsen (2006) Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues. J. Chromatography A. 1104: 291-298 [DOI: 10.1016/j.chroma.2005.11.045]

S.A. Mjøs & M. Solvang (2006) Patterns in volatile components over heated fish powders. Food Research International 39: 190-202 [DOI: 10.1016/j.foodres.2005.07.006]

S.A. Mjøs (2005) Properties of trans isomers of eicosapentaenoic acid and eicosahexaenoic acid methyl esters on cyanopropyl stationary phases. J. Chromatography A. 1100: 185-192 [DOI: 10.1016/j.chroma.2005.09.079]

S.A. Mjøs (2004) Two-dimensional fatty acid retention indices. J. Chromatography A 1061: 201-209 [DOI: 10.1016/j.chroma.2004.11.025]

S.A. Mjøs (2004) The prediction of fatty acid structure from selected ions in electron impact mass spectra of fatty acid methyl esters. Eur. J. Lipid. Sci. Technol. 106: 550-560 [DOI: 10.1002/ejlt.200401013]

S.A. Mjøs (2004) Quantification of linolenic acid isomers by gas chromatography/mass spectrometry and deconvolution of overlapping chromatographic peaks. Eur. J. Lipid Sci. Technol. 106: 307-318 [DOI: 10.1002/ejlt.200300909]

 

S.A. Mjøs (2003) Identification of fatty acids in gas chromatography by application of different temperature and pressure programs on a single capillary column. J. Chromatography A, 1015: 151-161 [DOI: 10.1016/S0021-9673(03)01240-8]

S.A. Mjøs (2003) Spectral transformations for deconvolution methods applied on gas chromatography-mass spectrometry data. Analytica Chimica Acta 488: 231-241 [DOI: 10.1016/S0003-2670(03)00679-2]

S.A. Mjøs & Pettersen (2003) A rapid method for the analysis of hydrogenated fats by GC with IR detection. J. Am. Oil Chem. Soc. 80: 839-846 [DOI: 10.1007/s11746-003-0783-0]

S.A. Mjøs & J. Pettersen (2003) Determination of trans double bonds in polyunsaturated fatty acid methyl esters from their electron impact mass spectra. Eur. J. Lipid Sci. Technol. 105: 156-164 [DOI: 10.1002/ejlt.200390031]

Nenseter, Østerud, Larsen, Strøm, Bergei, Hewitt, Holven,  Hagve, Mjøs, Solvang, Pettersen, Opstvedt, Ose. (2000) Effect on Norwegian fish powder on risk factors for coronary heart disease among hypercholesterolemic individuals.  Nutr. Metab. Cardiovasc. Dis. 10: 323-330.