NORBRAIN3: New 2-photon microscope with simultaneous 2-photon uncaging
We multiply our 2-photon microscopy power with a new custom-built microscope for 3D scanning with simultaneous photo-stimulation and 2-photon uncaging. Available soon as a core facility for researchers in Norway.
Main content
2-photon (2P) microscopy is a powerful technique that permits visualization and interrogation of complex neural tissue in 3 dimensions, with detailed subcellular resolution and minimal phototoxicity.
Neurons receive and transform signals arising from hundreds and thousands of synaptic inputs, and it is technically challenging to investigate the molecular and cellular mechanisms at the necessary spatial and temporal resolution.
By combining 2P microscopy with 2P neurotransmitter uncaging, it becomes possible to precisely activate single and multiple synapses to investigate how dendrites transform synaptic inputs and how neurons and neural microcircuits process information.
This new instrument will allow 2P-based structural and functional imaging and simultaneous neurotransmitter uncaging in live (in vitro) brain tissue slices, combined with multi-electrode patch-clamp recording. It was designed, delivered and built by Independent NeuroScience Services (INSS).
Funding for this system is from NORBRAIN - the Norwegian Brain Initiative funded by the Research Council of Norway, a large-scale infrastructure program to provide nation-wide access to cutting edge neurotechnology. After testing, the system will be accessible for researchers across Norway.
Stay tuned for more information.
