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The Laboratory Animal Facility

3R - Replacement, Reduction and Refinement of animal experiments

What are the 3Rs? How shall I relate to them in the application for animal experimentation?

Content

Replacement

Anyone who applies to do experiments involving animals in research, testing or education must document that there are no alternatives to use of animals.

An important control-question to ask yourself is:

Have there been any developments in your scientific field (including the development/validation of new in vitro or in silico techniques) which would replace some or all of the use of animals?

The Animal facility at University of Bergen has prepared a special room for training on procedures without use of animals

Reduction

Control questions

  • Has the project/experimental design been revisited to enable any further reduction in predicted animal use?
  • Were the models used still the most appropriate?
  • Were the numbers of animals used appropriate for statistical analysis (too many/too few)?
  • Could different approaches reduce further animal use?

Here you can read more about reduction and design of animal experiments 

Refinement

Refinement includes all means and actions to reduce or eliminate negative impact on the animals.

Refinement also includes means and actions that optimize the animal welfare.

Refinement is also a tool to improve quality of research. Experiment scan be performed faster, cheaper, less risk and with better quality.

Refinement is a tool to promote both good science and good animal welfare

 

In the application of animal experiments you have to demonstrate you refinement actions. You have to list any refinements you introduced during the project to reduce harm to the animals.

List alle raffineringstiltak som introdusert i dette prosjektet for å redusere negativ innvirkning på dyrene

Control question:

  • Could negative impact be further reduced?
  • Could the procedures (for example administration/sampling routes; surgery) be further refined?
  • Could animal monitoring regimes be improved?
  • Were score sheets/welfare assessment protocols working well?
  • Could humane end-points be refined?
  • Could euthanasia methods be refined 

In the margin (to the right) there is a pdf of a presentation of different ways to refine animals experiments.

Refinement and reduction of breeding colonies

FELASA has produced guidelines for refinement of procedures relatet to breeding. They can be useful if you plan experiments and write ethical applications.

Reduction: Describe how you keep the colony at a minimum as wells as breeding strategy to reduce number of animals that cannot be used because of wrong genotype.

The alternative lab at the Lab Animal Facility

The Animal facility at University of Bergen has prepared a special room for training on procedures without use of animals

You can read more about it and how to get access here

Search for alternatives (Replacement, Reduction and Refinement)

Anyone who applies to do experiments involving animals in research, testing or education must document that there are no alternatives to use of animals.

The researcher must perform a search for alternatives and document this in the application. The search must include keywords that are related to the animal model, procedures used as well as the keywords related to the research field.

Alternatives to animals are poorly represented in Pubmed

Poor documentation of or too narrow search for alternatives is a common reason for delay of approval.

Serach for alternatives must include alternatives to Pubmed

Norecopa has developed at «super-search engine» the 3R guide that combines different databases. This link will take you directly to the 3R guide.

In 3R guide you also find relevant guidelines, information-centers, discussion for a, journals as well as other 3R-surces.

See more about 3R and alternatives on this page about norecopa.

Other sources for search for alternatives

Below you find links to more sources for alternatives

http://www.lib.ucdavis.edu/dept/animalalternatives/

http://altweb.jhsph.edu

http://oslovet.norecopa.no/dokument.aspx?dokument=79

http://www.nc3rs.org.uk/

http://3rs.ccac.ca/en/

http://www.frame.org.uk

 

There are also some journals with useful information

•ATLA

•Alternatives to Laboratory Animals

•Animal Welfare

•(UFAW)

•ILAR Journal

•Institute for Laboratory Animal Research - http://dels.nas.edu/ilar

•Laboratory Animals

•The Royal Society of Medicine Press

•Comparative Medicine

•American Association for Laboratory Animal Science (AALAS)

For further advice contact aurora.bronstad@k1.uib.no