Tissue Engineering

Vil 3D-printe deler av skjelettet ditt

The Tissue Engineering group, founded in 2006, is one of four research groups at the Department of Clinical Dentistry. The group, led by Prof. Kamal Mustafa, aims to address the shortage of tissues available for repair & regeneration. It includes a multidisciplinary team of scientists, clinicians & bioengineers working in close collaboration with national & international partners. Currently, we are developing functional engineered bone-tissue constructs, with combinations of cells, growth factors & the appropriate biomaterial scaffolds. The scope of our work includes all aspects of pre-clinical & clinical biomedical research.  Read more

The group has consistently received funding from national and international (including EU) agencies to partner and coordinate large research projects. Currently, four major projects are ongoing:

- Trond Mohn Foundation (StemBoneReg)              

- Research Council of Norway (NFR BEHANDLING)

- EU MAXIBONE                                                       

- Commercialising R&D results (NFR FORNY2020)

We also provide a Micro-CT Core Facility for both internal (UiB) and external users. Read more 

New funding
3D Bioplotter

10 Thon-millioner til beinbygging

Ten Thon-Millions to make bone


Keynote lecture at the EAO Congress, Lisbon, 2019

Prof. Mustafa presented a keynote lecture at the 28th annual EAO Congress titled "Are stem cells the implants of the future"

Cover image of Journal of Dental Research, October 2019 issue

Cover image of Journal of Dental Research, 2019 Oct issue

Tooth morphogenesis is not driven by proliferation but spatiotemporal change in cell shape regulated by actomyosin. Ph. D candidate Shuntaro Yamada’s previous work “Molar Bud-to-Cap Transition Is Proliferation Independent” conducted at King’s College London, UK, was published on The Journal of...

Niyaz Al-sharabi

ITI Research Grant to Postdoc Niyaz Al-sharabi

Niyaz Al-sharabi, a postdoc in the Tissue Engineering Research Group at IKO received the ITI Research Grant for 2019.The research project is titled "Modification of exosomes derived from Mesenchymal Stem Cell (MSC) for promoting bone tissue regeneration in vitro”.

Recent publications by the group:

Suliman S, Ali HRW, Karlsen TA, Amiaud J, Mohamed-Ahmed S, Layrolle P, Costea DE, Brinchmann JE, Mustafa K. Impact of humanised isolation and culture conditions on stemness and osteogenic potential of bone marrow derived mesenchymal stromal cells. Sci Rep. 2019 Nov 5;9(1):16031. doi: 10.1038/s41598-019-52442-9. PMID: 31690774

Hassan MN, Yassin MA, Suliman S, Lie SA, Gjengedal H, Mustafa K. The Bone Regeneration Capacity of 3D-Printed Templates in Calvarial Defect Models: A Systematic Review and Meta-Analysis. Acta Biomaterialia 91:1-23. doi: 10.1016/j.actbio.2019.04.017 (2019).

Jain S, Fuoco T, Yassin MA, Mustafa K, Wistrand AF. Printability and critical insight into polymer properties during direct- extrusion based 3D printing of medical grade polylactide and copolyesters. Biomacromolecules doi: 10.1021/acs.biomac.9b01112 (2019).

Fuoco T, Ahlinder A, Jain S, Mustafa K, Finne-Wistrand A. Poly(ε-caprolactone-co-p-dioxanone): a Degradable and Printable Copolymer for Pliable 3D Scaffolds Fabrication toward Adipose Tissue Regeneration. Biomacromolecules doi: 10.1021/acs.biomac.9b01126 (2019).

Ahlinder A, Fuoco T, Lopez AM, Yassin MA, Mustafa K, Finne-Wistrand A. Nondegradative additive manufacturing of medical grade copolyesters of high molecular weight and with varied elastic response. Accepted in J Applied Polymer Science (2019)

Munir A, Døskeland A, Avery SJ, Fuoco T, Mohamed-Ahmed S, Lygre H, Finne-Wistrand A, Sloan AJ , Waddington RJ, Mustafa K, Suliman S. Journal of Tissue Engineering 31;10:2041731419852703. doi: 10.1177/2041731419852703 (2019).

Sharma S, Xue Y, Xing Z, Yassin MA, Sun Y, Lorens JB, Finne-Wistrand A, Sapkota D, Mustafa K. Adenoviral mediated mono delivery of BMP2 is superior to the combined delivery of BMP2 and VEGFA in bone regeneration in a critical-sized rat calvarial bone defect. Bone Reports 11;10:100205. doi: 10.1016/j.bonr.2019.100205. (2019).  

Sanz M, Dahlin C, Apatzidou D, Artzi Z, Bozic D, Calciolari E, De Bruyn H, Dommisch H, Donos N, Eickholz P, Ellingsen JE, Haugen HJ, Herrera D, Lambert F, Layrolle P, Montero E, Mustafa K, Omar O, Schliephake H. Biomaterials and regenerative technologies used in bone regeneration in the craniomaxillofacial region: Consensus report of group 2 of the 15th European Workshop on Periodontology on Bone Regeneration. J Clin Periodontol 46 Suppl 21:82-91. doi: 10.1111/jcpe.13123 (2019).

Yassin MA, Fuoco T, Mohamed-Ahmed S, Mustafa K, Finne-Wistrand A. 3D and Porous RGDC-Functionalized Polyester-Based Scaffolds as a Niche to Induce Osteogenic Differentiation of Human Bone Marrow Stem Cells. Macromol Biosci. 2019 May 3:e1900049. doi: 10.1002/mabi.201900049 (2019).

Rojewski MT, Lotfi R, Gjerde C, Mustafa K, Veronesi E, Ahmed AB, Wiesneth M, Körper S, Sensebé L, Layrolle P, Hellem S, Schrezenmeier H. Translation of a standardized manufacturing protocol for mesenchymal stromal cells: A systematic comparison of validation and manufacturing data.  Cytotherapy 21: 468-482 (2019).

Ojansivu M, Rashad A, Ahlinder AE, Massera J, Mishra A, Syverud K, Finne-Wistrand A, Miettinen S, Mustafa K. Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells. Biofabrication. doi: 10.1088/1758-5090/ab0692 (2019).

Rashad A, Suliman S, Mustafa M, Pedersen TO, Campodoni E, Sandri M, Syverud K, Mustafa K. Inflammatory Responses and Host Tissue Reactions to Wood-Based Nanocellulose Scaffolds. Materials Science & Engineering C Mater Biol Appl. 97:208-221 (2019).

Shanbhag S, Suliman S, Pandis N, Stavropoulos A, Sanz M, Mustafa K. Cell therapy for orofacial bone regeneration: a systematic review and meta-analysis. J Clin Periodontol doi: 10.1111/jcpe.13049 (2019).

Campodoni E, Heggset EB, Rashad A, Ramírez-Rodríguez GB, Mustafa K, Syverud K, Tampieri A, Sandri M. Polymeric 3D scaffolds for tissue regeneration: Evaluation of biopolymer nanocomposite reinforced with cellulose nanofibrils. Materials Science & Engineering C 94: 867 – 878. doi: 10.1016/j.msec (2019).

Gjerde CG, Mustafa K et al 2018. Cell therapy induced regeneration of severely atrophied mandibular bone - Part II: Clinical outcome. Stem Cell Res Ther. 9;9(1):213. doi: 10.1186/s13287-018-0951-9 (2018).

Rashad A, Mohamed-Ahmed S, Ojansivu M, Berstad K, Yassin MA, Kivijärvi T, Heggset EB, Syverud K, Mustafa K. Coating 3D Printed Polycaprolactone Scaffolds with Nanocellulose Promotes Growth and Differentiation of Mesenchymal Stem Cells. Biomacromolecules. 12: 4307-4319. doi: 10.1021/acs.biomac.8b01194. (2018).

Weigel T, Schmitz T, Pfister T, Gaetzner S, Jannasch M, Al-Hijailan R, Schürlein S, Suliman S, Mustafa K, Hansmann J. A three-dimensional hybrid pacemaker electrode seamlessly integrates into engineered, functional human cardiac tissue in vitro. Sci Rep. 28: 14545. doi: 10.1038/s41598-018-32790-8. (2018).

Mohamed-Ahmed S, Fristad I, Lie SA, Suliman S, Mustafa K, Vindenes H, Idris SB. Adipose-derived and bone marrow mesenchymal stem cells: a donor-matched comparison. Stem Cell Res Ther. 19:168. doi: 10.1186/s13287-018-0914-1. (2018).

Ibrahim M, Schoelermann J, Mustafa K, Cimpan MR. TiO2 nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast-like cells in a size dependent manner. J Biomed Mater Res A. 30. doi: 10.1002/jbm.a.36448 (2018).

Gharaei MA, Xue Y, Mustafa K, Lie SA, Fristad I. Human dental pulp stromal cell conditioned medium alters endothelial cell behaviour. Stem Cell Res Ther. 21;9(1):69. doi: 10.1186/s13287-018-0815-3 (2018).

Ibrahim M, Xue Y, Ostermann M, Sauter A, Steinmueller-Nethl D, Schweeberg S, Krueger A, Cimpan MR, Mustafa K. In vitro cytotoxicity assessment of nanodiamond particles and their osteogenic potential. J Biomed Mater Res, Feb 16. doi: 10.1002/jbm.a.36369 (2018).

Sharma S, Sapkota D, Xue Y, Rajthala S, Yassin MA, Finne-Wistrand A, Mustafa K. Delivery of VEGFA in bone marrow stromal cells seeded in copolymer scaffold enhances angiogenesis, but is inadequate for osteogenesis as compared with the dual delivery of VEGFA and BMP2 in a subcutaneous mouse model. Stem Cell Res Ther, 31;23. doi: 10.1186/s13287-018-0778-4 (2018).

Helgeland E, Shanbhag S, Pedersen TO, Mustafa K, Rosén A. Scaffold-based TMJ tissue regeneration in experimental animal models: a systematic review. Tissue Eng Part B Rev, Feb 5. doi: 10.1089/ten.TEB.2017.0429 (2018).

Sharma S, Sapkota D, Xue Y, Sun Y, Finne-Wistrand A, Bruland O, Mustafa K. Adenoviral mediated delivery of VEGFA in BMSC promotes angiogenesis, but does not enhance osteogenesis. 2018, submitted manuscript.

Gjerde CG, Santis DD,  Dominici M et al…, Mustafa K, Veronesi E. Autologous porcine bone marrow mesenchymal cells for reconstruction of a resorbed alveolar bone: a preclinical model in mini-pigs. Int J Stem Cell Res Ther, 4:050, DOI: 10.23937/2469-570X/1410050 (2017).

Rashad A, Mustafa K, Heggset EB, Syverud K. Cytocompatibility of Wood-Derived Cellulose Nanofibril Hydrogels with Different Surface Chemistry. Biomacromolecules 2017 Apr 10;18(4):1238-1248. doi: 10.1021/acs.biomac.6b01911. Epub (2017).

Yassin MA, Mustafa K et el…. A Copolymer Scaffold Functionalized with Nanodiamond Particles Enhances Osteogenic Metabolic Activity and Bone Regeneration. Macromolecular Bioscience 2017, Jan 24. doi: 10.1002/mabi.201600427, (2017).

Shanbhag S, Suliman S, Stavropoulos A, Hervig T, Mustafa K. Efficacy of humanized mesenchymal stem cell cultures for bone tissue engineering: a systematic review with a focus on platelet-derivatives. Accepted in Tissue Engineering Part B (2017).

Lynds R, Lyu C, Lyu G-W, Shi X-Q, Rosen A, Mustafa K, Shi T-J. Neuronal plasticity of trigeminal ganglia in mice following nerve injury. J pain Research 2017, 2017 Feb 9;10:349-357. doi: 10.2147/JPR.S120092. (2017).

Shanbhag S, Pandis N, Mustafa K, Nyengaard JR, Stavropoulos A. Bone tissue engineering in oral peri-implant defects in preclinical in vivo research: a systematic review and meta-analysis. J Tissue Eng Regen Med, doi: 10.1002/term.2412. (2017).

Bartaula S, Bolstad AI, Mustafa K, Pedersen TO. Secretome of mesenchymal stem cells grown in hypoxia accelerates wound healing and vessel formation in vitro. Int J Stem Cell Res Ther 2017, 4:045 DOI: 10.23937/2469-570X/1410045 (2017).

Mohamed HG, Mustafa K, Ibrahim SO, Åstrøm AN. Dietary habits, oral impact on daily performance and type 2 diabetes: a matched case-control study from Sudan.  Health and Quality of Life Outcomes. 15:111. doi: 10.1186/s12955-017-0686-9, (2017).

Shanbhag S, Pandis N, Mustafa K, Nyengaard JR, Stavropoulos A. Cell co-transplantation strategies for vascularized craniofacial bone tissue engineering: a systematic review and meta-analysis. Tissue Engineering, Part B, 23:101-117 (2017).  

Suliman S, Mustafa K, Krueger A, Steinmüller-Nethl D, Finne-Wistrand A, Osdal T, Hamza AO, Sun Y, Parajuli H, Waag T, Nickel J, Johannessen A-Ch, Cormack EM, Costea DE. Nanodiamond modified copolymer scaffolds affects tumour progression. Biomaterials 2016, 95:11-21.

Suliman S, Sun Y, Pedersen TO, Xue Y, Nickel J, Finne-Wistrand A, Steinmüller-Nethl D, Krueger A, Costea DE, Mustafa K. Host response and degradation of copolymer scaffolds functionalised with nanodiamonds and BMP2. Adv. Healthc. Mater., 2016, 5 (6), 730-742.   

Galli S, Andersson M, Jinno Y, Karlsson J, He W, Xue Y, Mustafa K, Wennerberg A, Jimbo R. Magnesium release from mesoporous carriers on endosseus implants does not influence bone maturation at 6 weeks in rabbit bone. J Biomed Mater Res B Appl Biomater. 2016 13. doi: 10.1002/jbm.b.33752.

Shanbhag S, Pandis N, Mustafa K, Nyengaard JR, Stavropoulos A. Alveolar bone tissue engineering in critical-size defects of experimental animal models: a systematic review and meta-analysis. (2016), J Tissue Eng Regen Med, 2016 doi: 10.1002/term.2198. Review

Bartaula S, Pedersen TO, Finne-Winstrand A, Bolstad AI, Mustafa K. Angiogenic and Immunomodulatory Properties of Endothelial and Mesenchymal Stem Cells. Tissue Eng Part A. (2016), 22(3-4), 244-252, DOI: 10.1089/ten.tea.2015.0316

Sharma S, Sapkota D, Xue Y, Sun Y, Finne-Wistrand A, Bruland O, Mustafa K. Adenoviral Mediated Expression of BMP2 by Bone Marrow Stromal Cells cultured in 3D Copolymer Scaffolds Enhances Bone Formation. PLoS ONE (2016). 2016 25;11(1):e0147507. doi: 10.1371/journal.pone.0147507. eCollection 2016.

Yassin MA, Leknes KN, Pedersen TO, Xing Z, Sun Y, Lie SA, Finne-Wistrand A, Mustafa K. Surfactant Tuning of Hydrophilicity of Porous Degradable Copolymer Scaffolds Promotes Cellular Proliferation and Enhances Bone Formation. J Biomed Mater Res A 2016, doi: 10.1002/jbm.a.35741

Al-Sharabi N, Xue Y, Mustafa M, Udea M, Xue Y, Mustafa K, Fristad I. Conditioned media from human bone marrow stromal cells attenuate initial inflammatory reactions in dental pulp tissue. Dental Traumatology 2016; doi: 10.1111/edt. 12277.

Mohamed HG, Idris SB, Mustafa M, Ahmed MF, Åstrøm AN, Mustafa K, Ibrahim SO. Influence of type 2 diabetes on prevalence of key periodontal pathogens, salivary matrix metalloproteinases and bone remodeling markers in adults with and without chronic periodontitis. Int J Dent. 2016;2016:6296854. doi: 10.1155/2016/6296854. Epub 2016

Dagmar F Bunæs DF, Lie SA, Aastrøm AN, Mustafa K, Leknes KN. Site-specific effect of smoking following 12 months of supportive periodontal therapy, J Clin Periodontol. 2016 Dec;43(12):1086-1093. doi: 10.1111/jcpe.12619. (2016).

Masahito F, Yamamoto A, Hibi H, Ueda M, Fristad I, Mustafa K. Conditioned media from hypoxic-cultured human dental pulp cells promotes distraction osteogenesis healing through blood vessel enrichment. J Tissue Eng Regen Med. 2015. doi: 10.1002/term.2109. (2015).

Al-Sharabi N, Xue Y, Udea M, Mustafa K, Fristad I. Influence of bone marrow stromal cell secreted molecules on pulpal and periodontal healing in replanted immature rat molars. Dental Traumatology 2015; doi: 10.1111/edt. 12246.

Suliman S, Xing Z, Wu X, Xue Y, Pedersen TO, Sun Y, Døskeland AP, Nickel J, Waag T, Lygre H, Finne-Wistrand A, Steinmüller-Nethl D, Krueger A, Mustafa K. Release and bioactivity of bone morphogenetic protein-2 are affected by scaffold binding techniques in vitro and in vivo. J Control Release. 2015 10;197:148-57.

Suliman S, Parajuli H, Sun Y, Johannessen AC, Finne-Winstrand A, McCormack E, Mustafa K, Daniela Costea. Establishment of a bioluminescence model for microenvironmentally induced oral carcinogenesis with implications for screening bioengineered scaffolds. Head and Neck (2015, Aug 14. doi: 10.1002/hed.24187).

Dagmar F Bunæs DF, Lie SA, Enersen M, Aastrøm AN, Mustafa K, Leknes KN. Site specific detrimental tissue responses to cigarette smoking following non-surgical and surgical periodontal therapy. J Clin Periodontol. 2015 26. doi: 10.1111/jcpe.12462 (2015).

Mohamed HG, Idris SB, Ahmed MF, Åstrøm AN, Mustafa K, Ibrahim SO, Mustafa M. Influence of type 2 diabetes on local production of inflammatory molecules in adults with and without chronic periodontitis: a cross-sectional study. BMC Oral Health 27. doi: 10.1186/s12903-015-0073-z (2015).

Yassin MA, Leknes KN, Pedersen TO, Xing Z, Sun Y, Lie SA, Finne-Wistrand A, Mustafa K. Cell seeding density is a critical determinant for copolymer scaffolds-induced bone regeneration. J Biomed Mater Res A. (2015, DOI: 10.1002/jbm.a.35505).

Mohamed HG, Idris SB, Mustafa M, Ahmed MF, Åstrøm AN, Mustafa K, Ibrahim SO. Impact of Chronic Periodontitis on Levels of Glucoregulatory Biomarkers in Gingival Crevicular Fluid of Adults with and without Type 2 Diabetes. PLoS ONE 10: e 0127660 (2015).

Westas E, Svanborg LM, Wallin P, Wennerberg A, Mustafa K, Andersson M. Using QCM-D to study adhesion of human gingival fibroblasts on implant surfaces. J Biomed Mater Res A. (2015), Mar 16. doi: 10.1002/jbm.a.35458.

Sun Y, Finne-Wistrand A, Xing Z, Yassin M, Yamamoto A, Mustafa K, Steinmüller-Nethl D, Krueger A, Albertsson A-C. Reinforced degradable biocomposite by homogenously distributed functionalized nanodiamond particles. Macromolecular Materials and Engineering 300: 8436-447 (2015).

Skodje A, Idris SB, Sun Y, Bartaula S, Mustafa K, Finne-Wistrand A, Wikesjö UM, Leknes KN. Biodegradable Polymer Scaffolds Loaded with Low-Dose BMP-2 Stimulate Cell Differentiation. J Biomed Mater Res A 2015 Jun;103:1991-8. doi: 10.1002/jbm.a.35334.

Bartaula S, Pedersen TO, Blois AL, Papadakou P, Finne-Winstrand A, Xue Y, Bolstad AI, Mustafa K. Leukocyte transmigration into tissue-engineered constructs is influenced by endothelial cells through toll-like receptor signaling. Stem Cell Res Ther. 2014 Dec 20;5(6):143.

Galli S, Naito Y, Karlsson J, He W, Miyamoto I, Xue Y, Andersson M, Mustafa K, Wennerberg A, Jimbo R. Local release of magnesium from mesoporous TiO2 coatings stimulates the peri-implant expression of osteogenic markers and improves osteoconductivity in vivo. Acta Biomaterial 2014, Aug 18. pii: S1742-7061(14)00347-X. doi: 10.1016/j.

Al-Sharabi N, Xue Y, Fujio M, Ueda M, Gjerde C, Mustafa K, Fristad I. Bone marrow stromal cell paracrine factors direct osteo/odontogenic differentiation of dental pulp cells. Tissue Eng Part A. 2014 May 11.

Breding K, Jimbo R, Hayashi M, Xue Y, Mustafa K, Andersson M. The effect of hydroxyapatite nanocrystals on osseointegration of titanium implants: an in vivo rabbit study. Int J Dent. (2014): 171305.

Yang Sun Y, Xing Z, Xue Y, Mustafa K, Finne-Wistrand A, Albertsson A-C. Surfactant as a critical factor when tuning the hydrophilicity in three-dimensional polyester-based scaffolds: impact of hydrophilicity on their mechanical properties and the cellular response of human osteoblast-like cells. Biomolecules. (2014, Feb).

Pedersen TO, Blois A, Xue Y, Xing Z, Sun Y, Finne-Wistrand A, Lorens JB, Fristad I, Leknes KN, Mustafa K. Mesenchymal stem cells induce endothelial cell quiescence and promote capillary formation. Stem Cell Res Ther. 2014, Feb 17;5(1):23. doi: 10.1186/scrt412.

Hayashi M, Jimbo R, Xue Y, Mustafa K, Andersson M, Wennerberg A. Photocatalytically induced hydrophilicity influences bone remodeling at longer healing periods: a rabbit study. Clin Oral Implants Research 2014 Jun;25(6):749-54. doi: 10.1111/clr.12138.

Mohamed H, Idris SB, Ahmed MF, Egil O, Mustafa K, Nordrehaug A. Association between oral health status and Type 2 diabetes mellitus among Sudanese adults: a matched case-control study. PLoS ONE 2014;9(4):e94575.