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Nuclear lipids: biomolecular interactions and function

Current projects overview

Protein-phospholipids interaction networks in health and diseases

1. Identifying phosphoinositide interacting nuclear proteins to understand their function

In order to decipher phosphoinositide (PI) nuclear functions, we have established quantitative proteomic methods to enrich for and to identify nuclear PI effector proteins. Using this method, we have identified nuclear proteins interacting with phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P2) and PtdIns(3,4,5)P3. Current projects include further analyses of these potential interactions:

  • Biochemical validation of the interactions
  • Role of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 in the function of these proteins
  • Mechanisms of regulation of these interactions

2. Mapping changes in phosphoinositide-protein interactomes in diseases

Our goal is to map which protein-PI networks are remodeled in pathological states where the PI metabolic pathways are known to be altered.

  • Protein-3P-PI networks are also under investigation in endometrial cancer (PhD position finansed by Kreftforeningen, 2012-2015).
  • Protein-PI networks in obesity and insulin resistance are under investigation under a project entitled "Characterisation of changes in protein-lipid interaction networks in insulin resistance and evaluation of specific networks as potential therapeutic targets". (Helse Vest 2011-2013.