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Nuclear lipids: biomolecular interactions and function

Current projects overview

Protein-phospholipids interaction networks in health and diseases

1. Identifying phosphoinositide interacting nuclear proteins to understand their function

In order to decipher phosphoinositide (PI) nuclear functions, we have established quantitative proteomic methods to enrich for and to identify nuclear PI effector proteins. Using this method, we have identified 28 nuclear proteins interacting with phosphatidylinositol (4,5) bisphosphate (PtdIns(4,5)P2), one of the 7 existing PIs.

Current projects include further analyses of these  potential interactions:

• Biochemical validation of the interactions

• Role of PtdIns(4,5)P2 in the function of these proteins

• Mechanism of regulation of these interactions

• Effect of PtdIns(4,5)P2 on protein structure

 

2. Mapping changes in phosphoinositide-protein interactomes in diseases

Our current goal is to map which protein-PI networks are remodeled in pathological states where the PI metabolic pathways are known to be altered.

  • Protein-PI networks in obesity and insulin resistance are under investigation under a project entitled "Characterisation of changes in protein-lipid interaction networks in insulin resistance and evaluation of specific networks as potential therapeutic targets", financed by Helse Vest in 2011. In collaboration with Professor Gunnar Mellgren

 

  • Protein-3P-PI networks are also under investigation in endometrial cancer (PhD position finansed by Kreftforeningen, 2012-2015), in collaboration with Professor Helga B Salvesen.

 

Reference:

1. AE Lewis, L Sommer, MØ Arntzen, Y Strahm, NA Morrice, N Divecha and CS D’Santos (2011). Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction. Molecular & Cellular Proteomics. 10(2):M110.003376. doi:10.1074/mcp.M110.003376. IF: 8.8