Brenk lab

Pushing structure-based design for new antibiotics

An Experimental Toolbox for Structure-Based Hit Discovery for P. Aeruginosa FabF, a Promising Target for Antibiotics

A biochemical toolbox
Ludvik O. Espeland

Main content

Our recent preprint has just been published on ChemRxviv. The article is about FabF, which is an attractive target for new antibiotics. We describe an experimental toolbox to support structure-based drug design efforts for FabF from P. aeruginosa. The toolbox contains two types of binding assays (bio-layer interferometry and NMR assays) and robust conditions for soaking FabF crystals. We demonstrate that this toolbox is fit-for-purpose by validating hits obtained from virtual screening.